Elucidation of the metalloprotease-dependent onset of bloodcirculation

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370076
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: SEHARA Atsuko 京都大学, 再生医科学研究所, 教授 (60209038)
• Project Period (FY): 2010 – 2012
• Keywords: メタロプロテアーゼ / ADAM8 / 造血 / 血液循環 / 赤芽球 / 血管 / ゼブラフィッシュ / 細胞間接着

Research Abstract

We succeeded to observe the onset of erythrocyte circulation in livingzebrafish embryos. We could show that the start of blood circulation requires detachment oferythrocytes from blood vessels, in which process ADAM8 expressed in erythrocytes participate.Moreover, erythrocytes-blood vessel detachment dependent on ADAM8 was involved in the bloodvessel development. ADAM8 is expressed in inflammatory cells such as neutrophils and monocytesduring muscle regeneration in mice, and this protease is required for the removal of injuredmuscle.

Research Products

• [Journal Article] Roles andFunctions of Meltrin beta / ADAM19. (2013)
  • Author(s): Atsuko Sehara-Fujisawa
  • Journal Title: Handbookof Proteolytic Enzymes, 3rd Edition (Editor inChief: Rawlings & Salvesen)
  • Peer Reviewed
• [Journal Article] Activity-dependentProteolytic Cleavage of Neuroligin 1Neuron (2012)
  • Author(s): Suzuki, K.,et al
  • Volume: 76(2)
  • Pages: 410-22
  • DOI: DOI:10.1016/j.neuron.2012.10.003
  • Peer Reviewed
• [Journal Article] ADAM12 produces by tumorcells rather than stromal cells acceleratesbreast tumor progression (2011)
  • Author(s): rohlich, C.,et al
  • Journal Title: Mol. Cancer Res Volume: 9(11) Pages: 1449-61
  • DOI: DOI:10.1158/1541-7786.MCR-11-0100
  • Peer Reviewed
• [Journal Article] ADAM8プロテアーゼによる血液循環開始の制御、II.冠循環の基礎 冠循環系の生理学、 (2011)
  • Author(s): 飯田敦夫、瀬原淳子
  • Journal Title: 日本臨牀(日本臨牀社) Volume: 69巻、増刊号7 Pages: 37-41
• [Journal Article] ADAM8による血液循環開始の制御 (2010)
  • Author(s): 飯田敦夫、瀬原淳子
  • Journal Title: 生化学 Volume: 82(10) Pages: 921-930
• [Journal Article] Metalloprotease-DependentOnset of Blood Circulation In Zebrafish (2010)
  • Author(s): Iida, A., et al
  • Journal Title: Current Biol Volume: 20(12) Pages: 1110-1116
  • DOI: DOI:10.1016/j.cub.2010.04.052.
  • Peer Reviewed
• [Presentation] Exploring roles of ADAMproteases in development using zebrafish (2013)
  • Author(s): Atsuko Sehara
  • Organizer: 国際高等研究所研究プログラム「ゲノム工学とイメージングサイエンスに基づく生命システム研究の新展開」2012年度第1回研究会
  • Place of Presentation: 京都府
  • Year and Date: 2013-02-23
• [Presentation] 血液の発生と挙動におけるADAM8の役割 (2012)
  • Author(s): 西邨大吾、飯田敦夫、坂口和弥、杉山立樹、長澤丘司、千葉彩乃、望月直樹、バーツ.J.W.、瀬原淳子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡県
  • Year and Date: 2012-12-13
• [Presentation] ゼブラフィッシュの筋維持における増殖因子の役割とその制御 (2012)
  • Author(s): 瀬原淳子
  • Organizer: 第26回日本宇宙生物科学
  • Place of Presentation: 徳島県
  • Year and Date: 2012-09-28
• [Presentation] Exploring Roles ofADAM Proteases in Development usingZebrafish (2012)
  • Author(s): Atsuko Sehara-Fujisawa
  • Place of Presentation: Singapore, Singapore
  • Year and Date: 2012-07-26
• [Presentation] 生きたゼブラフィッシュを用いてADAMプロテアーゼの役割と機能に迫る (2012)
  • Author(s): 瀬原淳子
  • Organizer: 第12回日本抗加齢医学会総
  • Place of Presentation: 神奈川県
  • Year and Date: 2012-06-24
• [Presentation] 異なる細胞間の相互作用におけるADAMプロテアーゼの役割 (2012)
  • Author(s): 瀬原淳子
  • Organizer: 文部科学省科学研究費補助金新学術領域研究「動植物に共通するアロ認証機構の解明」シンポジウム「細胞間相互認識とアロ認証」
  • Place of Presentation: 茨城県
  • Year and Date: 2012-01-11
• [Presentation] 生きたゼブラフィッシュを用いて血管形成の新しいメカニズムに迫る (2011)
  • Author(s): Atsuo Iida, Kazuya Sakaguchi, DaigoNishimura, Anna Tomozawa, Atsuo Kawahara,Atsuko Sehara-Fujisawa
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都府
  • Year and Date: 2011-09-21
• [Presentation] A Role of ADAM8 inthe Onset of Blood Circulation (2011)
  • Author(s): Atsuo Iida, Kazuya Sakaguchi, AnnaTomozawa, Daigo Nishimura, Atsuo Kawahara,Atsuko Sehara-Fujisawa
  • Organizer: KEY Forum inDevelopmental Biology and RegenerativeMedicine-Supported by Global COE and IMEG
  • Place of Presentation: Kumamoto University熊本県
  • Year and Date: 2011-09-09
• [Presentation] Roles of Meltrinbeta (ADAM19) in development of peripheralnervous system. (2011)
  • Author(s): Atsuko Sehara-Fujisawa
  • Organizer: The 5th Asia-OseaniaZebrafish Meeting
  • Place of Presentation: 北京、中国
  • Year and Date: 2011-08-27
• [Presentation] Roles of ADAM19 innervous system development in zebrafish (2011)
  • Author(s): Atsuko Sehara-Fujisawa
  • Organizer: Gordon Research Conference"Metalloproteinase Research at theintersection of Basic Science and AppliedMedicine"
  • Place of Presentation: Smitnfield, RI, USA
  • Year and Date: 2011-08-07
• [Presentation] A roles of ADAM8 inthe onset of blood circulation (2011)
  • Author(s): Atsuko Sehara-Fujisawa
  • Organizer: The 7th AsoInternational Meeting
  • Place of Presentation: 熊本県
  • Year and Date: 2011-07-30
• [Presentation] The Role of ADAM andRIP Proteases in Development and Beyond (2011)
  • Author(s): Atsuko Sehara-Fujisawa
  • Organizer: GordonResearch Conferences "Regulated Proteolysis ofCell Surface Proteins"
  • Place of Presentation: Davidson,USA
  • Year and Date: 2011-07-10
• [Presentation] Roles of ADAM proteins in spatialand temporal regulation of ectodomain sheddingevents (2010)
  • Author(s): 瀬原淳子
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県
  • Year and Date: 2010-12-09
• [Presentation] Metalloproteasedependent regulation of nerve regeneration (2010)
  • Author(s): Atsuko Sehara-Fujisawa
  • Organizer: Society for Developmental Biology 69th AnnualMeeting Jointly with Japanese Society ofDevelopmental Biologists
  • Place of Presentation: Albuquerque, USA
  • Year and Date: 2010-08-05
• [Presentation] Metalloprotease-Dependent Onset of BloodCirculation In Zebrafish (2010)
  • Author(s): Atsuo Iida, Kazuya Sakaguchi, Kiyoaki Sato,Hidetoshi Sakurai, Daigo Nishimura, Aya Iwaki,Makoto Kobayashi, Shigenobu Yonemura, AtsuoKawahara, and Atsuko Sehara-Fujisawa
  • Organizer: 2nd Joint Meeting ofthe French and Japanese Societies forDevelopmental Biology
  • Place of Presentation: Paris, France
  • Year and Date: 2010-05-16