2012 Fiscal Year Final Research Report
Molecular dynamics to restore/remodel neuromuscular connections during muscle growth and regeneration
Project/Area Number |
22380145
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Zootechnical science/Grassland science
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Research Institution | Kyushu University |
Principal Investigator |
TATSUMI Ryuichi 九州大学, 大学院・農学研究院, 准教授 (40250493)
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Co-Investigator(Kenkyū-buntansha) |
FURUSE Mitsuhiro 九州大学, 大学院・農学研究院, 教授 (30209176)
MIZUNOYA Wataru 九州大学, 大学院・農学研究院, 助教 (20404056)
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Research Collaborator |
RONALD E Allen 米国アリゾナ大学, 教授
JUDY E Anderson 加国マニトバ大学, 理学部, 教授
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Project Period (FY) |
2010 – 2012
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Keywords | 食肉 / 骨格筋 / 筋幹細胞 / 運動神経 / 神経軸索成長ガイダンス因子Sema3A / 肝細胞増殖因子(HGF) / 塩基性線維芽細胞増殖因子(FGF2) / トランスフォーミング増殖因子(TGF)-β |
Research Abstract |
Successful regeneration and remodeling of the neuromuscular connections are critical for restoring functional properties of contractile physiology of muscle fibers. While the spatiotemporal regulatory mechanisms coordinating these processes with myogenesis itself remain unclear, various neural factors including attractive and repulsive axon-guidance cue ligands and their receptors may be involved. Recently, we found that satellite cells, resident myogenic stem cells normally positioned beneath the basal lamina of mature muscle fibers, up-regulate a secreted neural chemorepellent semaphorin 3A (Sema3A) during early-differentiation period, upon acute muscle injury in vivo(Tatsumi et al., 2009). The current study encouraged the possible regulation of Sema3A expression by time-coordinated prevalence of extracellular up-regulator (HGF and FGF2) and down-regulator (TGF-β3) concentrations bathing differentiating satellite cells (myoblasts) in regenerating muscle tissue. By understanding this growth factor ligand-driving scenario, we will be able to design new procedures that specifically target regenerative motoneuritogenesis to ensure muscle growth and repair, contributing to the meat-animal production and human sports and health sciences aimed to enhance physical performance and medical therapies for neuromuscular disorders and age-related muscle atrophy
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Research Products
(34 results)
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[Journal Article] Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury2013
Author(s)
Sato, Y., Do, M.-K. Q.,Suzuki, T., Ohtsubo, H., Mizunoya, W., Nakamura, M., Furuse, M., Ikeuchi, Y., Anderson, J. E.and Tatsumi, R
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Journal Title
Animal Science Journal
Volume: 84
Pages: 185-189
DOI
Peer Reviewed
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[Journal Article] Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression2013
Author(s)
Suzuki, T., Do, M.-K. Q., Sato, U., Ojima, K., Hara, M., Mizunoya, W., Nakamura, M., Furuse, M., Ikeuchi, Y., Anderson, J. E.and Tatsumi, R
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Journal Title
International Journal of Biochemistry and Cell Biology
Volume: 45
Pages: 476-482
DOI
Peer Reviewed
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[Journal Article] Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle2012
Author(s)
Do, M.-K. Q., Suzuki, T., Gerelt, B., Sato, Y., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Tatsumi, R
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Journal Title
Animal Science Journal
Volume: 83
Pages: 712-717
DOI
Peer Reviewed
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[Journal Article] Calcium influx through a possible coupling of cation-channels impacts skeletal muscle satellite cell activation in response to mechanical stretch2012
Author(s)
Hara, M., Tabata, K., Suzuki, T., Do, M.-K. Q., Mizunoya, W., Nakamura, M., Nishimura., S., Tabata, S., Ikeuchi, Y., Sunagawa, K., Anderson, J. E., Allen, R. E., and Tatsumi, R.
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Journal Title
American Journal of Physiology-Cell Physiology
Volume: 302
Pages: C1741-C1750
DOI
Peer Reviewed
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[Journal Article] Growth factor regulation of neural chemorepellent Sema3A expression in satellite cell cultures2011
Author(s)
Do, M.-K. Q., Sato, Y., Shimizu, N., Suzuki, T., Shono, J.-I., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Tatsumi, R
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Journal Title
American Journal of Physiology-Cell Physiology
Volume: 301
Pages: C1270-C1279
DOI
Peer Reviewed
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[Journal Article] In vitromeasurement of postnatal changes in proliferating satellite cell frequency during rat muscle growth2011
Author(s)
Suzuki, T., Takaishi, H., Sakata, T., Do, M. Q., Hara, M., Sato, A., Mizunoya, W., Nishimura, T, Hattori, A., Ikeuchi, Y., and Tatsumi, R
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Journal Title
Animal Science Journal
Volume: 81
Pages: 245-251
DOI
Peer Reviewed
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[Presentation] Heparan/chondroitin sulfate chains may mediate HGF/FGF2-induced up-regulation of neural chemorepellent Sema3A in satellite cell cultures2012
Author(s)
Do, M.Q., Shimizu, N., Suzuki, T., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J.E.,and Tatsumi, R
Organizer
2012 FASEB Science Research Conference on "Skeletal Muscle Satellite & Stem Cells"
Place of Presentation
Barga, Lucca, Italy
Year and Date
20120812-0817
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[Presentation] differentiation of satellite cells2012
Author(s)
Suzuki, T., Ojima, K., Do, M.Q., Hara, M., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J.E., and Tatsumi, R
Organizer
2012 FASEB Science Research Conference on "Skeletal Muscle Satellite & Stem Cells"
Place of Presentation
Barga, Lucca, Italy
Year and Date
20120812-0817
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[Presentation] Mechano-sensing calcium-influx machinery that instigates skeletal muscle satellite cell activation2012
Author(s)
Tatsumi, R., Hara, M., Tabata, K., Suzuki, T., Do, M.-K. Q., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Allen, R. E
Organizer
2012 FASEB Science Research Conference on "Skeletal Muscle Satellite & Stem Cells"
Place of Presentation
Barga, Lucca, Italy
Year and Date
20120812-0817
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[Presentation] Mechano-biology of resident myogenic stem cells: implication in postnatal myogenesis and intramuscular motoneuritogenesis2010
Author(s)
Tatsumi, R.,Mizunoya, W., Ikeuchi, Y., Hattori, A., Anderson, J. E., and Allen, R. E
Organizer
Animal Science Congress 2010 of the Asian-Australian Association of Animal Production Societies (AAAP)
Place of Presentation
National Pingtung University of Science and Technology (NPUST), Pingtung, Taiwan
Year and Date
20100823-0827
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[Presentation] Possible implication of satellite cells in regenerative motoneuritogenesis: HGF and FGF2 upregulate neural chemorepellent Sema3A expression2010
Author(s)
Tatsumi, R.,Do, M.-K. Q., Shimizu, N., Sankoda, Y., Anderson, J. E., Sato, Y., Suzuki, T., Mizunoya, W., Ikeuchi, Y., and Allen, R. E.
Organizer
the 6thWorld Congress on Biomechanics (WCB) 2010, in conjunction with the 14thInternational Conference on Biomedical Engineering (ICBME) 2010 and the 5thAsian Pacific Conference on Biomechanics (APBiomech) 2010
Place of Presentation
Suntec Convention and Exhibition Center, Singapore
Year and Date
20100801-0806
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[Presentation] Possible implication of satellite cells in regenerative motoneuritogenesis: temporal coordination of HGF/FGF-2/TGF-beta may regulate neural chemorepellent Sema3A expression2010
Author(s)
Do, M.-K. Q., Shimizu, N., Sato, Y., Suzuki, T., Tatsumi, R.,Mizunoya, W., Ikeuchi, Y., Anderson, J. E., and Allen, R. E
Organizer
2010 FASEB Summer Research Conference on "Skeletal Muscle Satellite & Stem Cells"
Place of Presentation
Carefree, AZ, US
Year and Date
20100718-0723
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