Elucidation of the pathogenesis of dengue fever and dengue hemorrhagic fever based on viral and immunological analysis

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390093
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: KURANE Ichiro 国立感染症研究所, 副所長 (90278656)
• Co-Investigator(Kenkyū-buntansha): TAKASAKI Tomohiko 国立感染症研究所, ウイルス第一部室長 (20221351) ; MATSUTANI Takaji 和歌山県立医科大学, 医学部, 講師 (70372290) ; SUZUKI Ryuji 独立行政法人国立病院機構相模原病院臨床研究センター, 室長 (70373470)
• Project Period (FY): 2010 – 2012
• Keywords: ウイルス / 感染症 / 免疫学

Research Abstract

Dengue virus infection is one of the most important mosquito-borne virus infectious diseases in the world. We developed an animal model to analyze the pathogenesis of dengue fever, using common marmosets. Marmosets demonstrated high levels of viremia after dengue virus infection. Marmosets also demonstrated antibody responses, and hematological and biochemical changes similar to those detected in dengue fever patients. Immunological methods to detect cytokines and cellular markers were developed, in order to precisely analyze T cell immune responses in marmosets.

Research Products

• [Journal Article] Immune-related gene expression profile in laboratory common marmosets assessed by an accurate quantitative real-time PCR using selected reference genes. (2013)
  • Author(s): Fujii, Y., Kitaura, K., Matsutani, T., Shirai, K., Suzuki, S., Takasaki, T., Kumagai, K., Kametani, Y., Shiina, T., Takabayashi, S., Katoh, H., Hamada, Y., Kurane, I. and Suzuki, R
  • Journal Title: PLoS One Volume: 8(2) Pages: e56296
  • DOI: DOI:10.1371/journal.pone.0056296
• [Journal Article] Presence of viral genome in urine and development of hematuria and pathological changes in kidneys in common marmoset (Callithrix jacchus) after inoculation with dengue virus. (2013)
  • Author(s): Moi, M.L., Omatsu, T., Hirayama, T., Nakamura, S., Katakai, Y., Yoshida, T., Saito, A., Tajima, S., Ito, M., Takasaki, T., Akari, H. and Kurane, I
  • Journal Title: Pathogens Volume: 2 Pages: 357-393
• [Journal Article] Changes in hematological and serum biochemical parameters in common marmosets (Callithrix jacchus) after inoculation with dengue virus. (2012)
  • Author(s): Omatsu, T., Moi, M.L., Takasaki, T., Nakamura, S., Katakai, Y., Tajima, S., Ito, M., Yoshida, T., Saito, A., Akari, H. and Kurane, I
  • Journal Title: Journal of Medical Primatology Volume: 41(5) Pages: 289-296
  • DOI: DOI:10.1111/j.1600-0684.2012.00552.x
• [Journal Article] A new method for quantitative analysis of the T cell receptor V region repertoires in healthy common marmosets by microplate hybridization assay. (2012)
  • Author(s): Kitaura, K., Fujii, Y., Matsutani, T., Shirai, K., Suzuki, S., Takasaki, T., Shimada, S., Kametani, Y., Shiina, T., Takabayashi, S., Katoh, H., Ogasawara, K., Kurane, I. and Suzuki, R.
  • Journal Title: Journal of Imunological Methods Volume: 384(1-2) Pages: 81-91
  • DOI: DOI:10.1016/j.jim.2012.07.012.
• [Journal Article] Common marmoset (Callithrix jacchus) as a primate model of dengue virus infection: development of high levels of viremia and demonstration of protective immunity. (2011)
  • Author(s): Omatsu, T., Moi, M.L., Hirayama., T., Takasaki, T., Nakamura, S., Tajima, S., Ito, M., Yoshida, T., Saito, A., Katakai, Y., Akari, H. and Kurane, I
  • Journal Title: Journal of General Virology Volume: 92(Pt10) Pages: 2272-2280
  • DOI: DOI:10.1099/vir.0.031229-0
• [Journal Article] Increased positive selection pressure within the complementarity determining regions of the T-cell receptor β gene in New World monkeys. (2011)
  • Author(s): Matsutani, T., Fujii, Y., Kitaura, K., Suzuki, S., Tsuruta, Y., Takasaki, T., Ogasawara, K., Nishimoto, N., Kurane, I. and Suzuki, R
  • Journal Title: American Journal of Primatology Volume: 73 Pages: 1082-1992
  • DOI: DOI:10.1002/ajp.20976.
• [Journal Article] Comprehensive analysis and characterization of the TCR alpha chain sequences in the common marmoset (2010)
  • Author(s): Fujii, K., Matsutani, T., Kitaura, K., Suzuki, S., Itoh, T., Takasaki, T., Suzuki, R. and Kurane, I
  • Journal Title: Immunogenetics Volume: 41(5) Pages: 383-385
  • DOI: DOI:10.1007/s00251-010-0445-0
• [Presentation] コモンマーモセットにおけるリアルタイムPCRを用いた免疫関連遺伝子発現解析 (2013)
  • Author(s): 北浦一孝、藤井克樹、松谷隆治、白井顕治、鈴木さつき、高崎智彦、亀谷美恵、倉根一郎、鈴木隆二
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: つくば
  • Year and Date: 20130515-17
• [Presentation] コモンマーモセットにおけるTCRレパトア解析法の開発 (2012)
  • Author(s): 北浦一孝、藤井克樹、松谷隆治、白井顕治、鈴木さつき、高崎智彦、倉根一郎、鈴木隆二
  • Organizer: 第59回日本実験動物学会総会
  • Place of Presentation: 別府
  • Year and Date: 20120524-26
• [Presentation] 新世界ザルにおけるT細胞受容体β鎖遺伝子のCDR3領域における正の選択 (2011)
  • Author(s): 北浦一孝、松谷隆治、藤井克樹、白井顕治、鈴木さつき、高崎智彦、小笠原康悦、西本憲弘、倉根一郎、鈴木隆二
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20111127-29