Cytoprotective effects of bioactive molecules derived from bacteria in the intestinal biofilm

2013 Fiscal Year Final Research Report

• Project/Area Number: 22390148
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Asahikawa Medical College
• Principal Investigator: KOHGO Yutaka 旭川医科大学, 医学部, 教授 (10133183)
• Co-Investigator(Kenkyū-buntansha): ASHIDA Toshifumi 旭川医科大学, 医学部, 客員教授 (50261409) ; FUJIYA Mikihiro 旭川医科大学, 医学部, 准教授 (80322915) ; OHTAKE Takaaki 旭川医科大学, 医学部, 講師 (10359490) ; MAEMOTO Atsuo 旭川医科大学, 医学部, 客員教授 (40400113) ; UENO Nobuhiro 旭川医科大学, 医学部, 助教 (30436000)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: 炎症性腸疾患 / 腸内細菌 / バイオフィルム

Research Abstract

The present study identified the alterations in the microflora in the intestinal lumen and biofilms, and between healthy volunteers and IBD patients based on an analysis of human and mouse samples. This study showed that probiotics-derived effectors exhibited protective effects against intestinal injury due to intestinal inflammation. The functions were mediated by the interaction between the probiotic-derived effectors and transporters or adherent molecules. These results will be helpful for the development of a novel treatment for intestinal inflammations using probiotic-derived effectors.

Research Products

• [Journal Article] Infliximab extends the duration until the first surgery in patients with Crohn's disease (2013)
  • Author(s): Sakatani A, Fujiya M, Ito T, Inaba Y, Ueno N, Kashima S, Tominaga M, Moriichi K, Okamoto K, Tanabe H, Ikuta K, Ohtake T, Kono T, Furukawa H, Ashida T, Kohgo Y
  • Journal Title: Biomed Research International Volume: 2013 Pages: 879491
• [Journal Article] Reduction of E-cadherin by Human Defensin-5 in Esophageal Squamous Cells (2013)
  • Author(s): Nomura Y, Tanabe H, Moriichi K, Igawa S, Ando K, Ueno N, Kashima S, Tominaga M, Goto T, Inaba Y, Ito T, Ishida-Yamamoto A, Fujiya M, Kohgo Y
  • Journal Title: Biochem Bioph Res Co Volume: 439(1) Pages: 71-77
• [Journal Article] Competence and sporulation factor derived from Bacillus subtilis improves epithelial cell injury in intestinal inflammation via immunomodulation and cytoprotection (2012)
  • Author(s): Okamoto K, Fujiya M, Nata T, Ueno N, Inaba Y, Ishikawa C, Ito T, Moriichi K, Tanabe H, Mizukami Y, Chang EB, Kohgo Y
  • Journal Title: Int J Colorectal Diseases Volume: 27(8) Pages: 1039-46
• [Journal Article] Effect of Lactobacillus brevis SBC8803 on Gamma-Glutamyl Transferase in Japanese Habitual Drinkers: A Double-Blind, Placebo-Controlled Study (2012)
  • Author(s): Wakita Y, Shimizu C, Nakakita Y, Kaneda H, Kanda H, Segawa S, Shigyou T, Ohtake T, Fujiya M, Kohgo Y
  • Journal Title: Food and Nutrition Sciences Volume: 3(5) Pages: 678-684
• [Journal Article] Isoleucine, an essential amino acid, induces the expression of human ・ defensin 2 through the activation of the G-protein coupled receptor-ERK pathway in the intestinal epithelia (2012)
  • Author(s): Konno Y, Ashida T, Inaba Y, Ito T, Tanabe H, Maemoto A, Ayabe T, Mizukami Y, Fujiya M, Kohgo Y
  • Journal Title: Food and Nutrition Sciences Volume: 3(4) Pages: 548-555
• [Journal Article] Paneth cell regulate gut inflammation, thereby inducing chemotaxis of dendritic cells and cytokine production from epithelial cells (2012)
  • Author(s): Ito T, Ayabe T, Ishikawa C, Inaba Y, Tanabe H, Maemoto A, Kono T, Ashida T, Fujiya M, Kohgo Y
  • Journal Title: Tohoku Journal of ExperimentalMedicine Volume: 227(1) Pages: 39-48
• [Journal Article] Heat-killed body of Lactobacillus brevis SBC8803 ameliorates intestinal injury in a murine model of colitis by enhancing the intestinal barrier function (2011)
  • Author(s): Ueno N, Fujiya M, Segawa S, Nata T, Moriichi K, Tanabe H, Mizukami Y, Kobayashi N, Ito K, Kohgo Y
  • Journal Title: Inflammatory Bowel Diseases Volume: 17(11) Pages: 2235-2250
• [Journal Article] Probiotic-derived polyphosphate enhances the epithelial barrier function and maintains intestinal homeostasis through integrin-p38 MAPK pathway (2011)
  • Author(s): Segawa S, Fujiya M, Konishi H, Ueno N, Kobayashi N, Shigyo T, Kohgo Y
  • Journal Title: PloS One Volume: 6(8) Pages: e23278
• [Journal Article] Cytokine Regulation of OCTN2 Expression and Activity in Small and Large Intestine (2011)
  • Author(s): Fujiya M, Inaba Y, Musch MW, Hu S, Kohgo Y, Chang EB
  • Journal Title: Inflammatory Bowel Diseases Volume: 17(4) Pages: 907-916
• [Journal Article] The expression of the anti-microbial peptide α-defensin/cryptdins in intestinal crypts decreases at the initial phase of intestinal inflammation in a model of inflammatory bowel disease, IL-10 deficient mice (2010)
  • Author(s): Inaba Y, Ashida T, Ito T, Ishikawa C, Tanabe H, Maemoto A, Watari J, Ayabe T, Mizukami Y, Fujiya M, Kohgo Y
  • Journal Title: Inflammatory Bowel Diseases Volume: 16(9) Pages: 1488-1495
• [Journal Article] Musch MW, Chang EB. Daikenchuto, a Japanese traditional medicine, blocks DSS-induced intestinal inflammation and damage
  • Author(s): Ueno N, Hasebe T, Kaneko A, Yamamoto M, Wang Y, Fujiya M, Kohgo Y, Kono T
  • Journal Title: PloS One Volume: (in press)
• [Journal Article] Augmented hepatic Toll-like receptors by fatty acids trigger the pro-inflammatory state of nonalcoholic fatty liver disease in mice
  • Author(s): Sawada K, Ohtake T, Hasebe T, Abe M, Tanaka H, Ikuta K, Suzuki Y, Fujiya M, Hasebe C, Kohgo Y
  • Journal Title: Hepatology Research Volume: (in press)
• [Presentation] Long-term oral dietary administration of a new probiotic, Lactobacillus brevis SBC8803, alters gut the microbiota and ameliorates DSS-induced colitis in mice (2014)
  • Author(s): Ueno N, Fujiya M, Segawa S, Konishi H, Sakatani A, Tanaka K, Dokoshi T, Ando K, Kashima S, Inaba Y, Ito T, Moriichi K, Shigyo T, Kohgo Y
  • Organizer: DDW 2014 (AGA)
  • Place of Presentation: Chicago
  • Year and Date: 2014-05-06
• [Presentation] Probiotic-derived polyphosphate inhibits colon cancer progression through the inactivation of the ERK pathway and the induction of cancer cell apoptosis (2013)
  • Author(s): Sakatani A, Fujiya M, Konishi H, Segawa S, Kashima S, Ueno N, Tanaka K, Dokoshi T, Ando K, Tominaga M, Inaba Y, Ito T, Tanabe H, Moriichi K, Kohgo Y
  • Organizer: DDW 2013 (AGA)
  • Place of Presentation: Orland
  • Year and Date: 2013-05-21
• [Presentation] A probiotic-derived polyphosphate improves chronic inflammation and fibrosis via caveolin-dependent endocytosis (2013)
  • Author(s): Konishi H, Fujiya M, Segawa S, Kashima S, Ueno N, Tanaka K, Sakatani A, Dokoshi T, Ando K, Tominaga M, Inaba Y, Ito T, Tanabe H, Moriichi K, Kohgo Y
  • Organizer: DDW 2013 (AGA)
  • Place of Presentation: Orland
  • Year and Date: 2013-05-21
• [Presentation] Polyphosphate is a novel active molecule derived from Lactobacillus brevis that contributes to the maintenance of intestinal homeostasis and the improvement of intestinal injury in acute and chronic experimental colitis (2012)
  • Author(s): Fujiya M, Ueno N, Sakatani A, Dokoshi T, Ando K, Nomura Y, Kashima S, Tominaga M, Inaba Y, Ito T, Moriichi K, Kikuchi Y, Okamoto K, Tanabe H, Segawa S, Kobayashi N, Shigyo T, Kohgo Y
  • Organizer: DDW 2012 (AGA)
  • Place of Presentation: San Diego
  • Year and Date: 2012-05-20
• [Presentation] Chang EB. Activation of Intestinal Epithelial Autophagy as a Potential and Novel Mechanism of Probiotic Action in the Gut (2012)
  • Author(s): Inaba Y, Fujiya M, Musch MW, Boone D, Kohgo Y
  • Organizer: 7th Congress of ECCO
  • Place of Presentation: Barcelona
  • Year and Date: 2012-02-16
• [Presentation] Lactobacillus brevis Culture Supernatant Induces Cytoprotective Small Heat Shock Protein HSP27 and Increase Intestinal Barrier Function by Activating the p38 MAPK Pathway (2011)
  • Author(s): Segawa S, Fujiya M, Konishi H, Ueno N, Kobayashi N, Shigyo T, Kohgo Y
  • Organizer: 15th International Congress of Mucosal Immunology (ICMI2011)
  • Place of Presentation: Paris
  • Year and Date: 2011-07-05
• [Presentation] Activation of Intestinal Epithelial Autophagy as a Potential and Novel Mechanism of Probiotic Action in the Gut (2011)
  • Author(s): Inaba Y, Fujiya M, Musch MW, Boone DL, Kohgo Y, Chang EB
  • Organizer: DDW 2011 (AGA)
  • Place of Presentation: Chicago
  • Year and Date: 2011-05-10
• [Presentation] Competence and Sporulation Factor Derived From Bacillus Subtilis Improves Epithelial Cell Injury in Intestinal Inflammation via Immunomodulation and Cytoprotection (2010)
  • Author(s): Okamoto K, Fujiya M, Nata T, Ueno N, Inaba Y, Ishikawa C, Ito T, Moriichi K, Tanabe H, Mizukami Y, Chang EB, Kohgo Y
  • Organizer: DDW 2010 (AGA)
  • Place of Presentation: New Orleans
  • Year and Date: 2010-05-05
• [Presentation] Lactobacillus Brevis SBC8803 Culture Supernatant Induces Cytoprotective Small Heat Shock Protein HSP27 in Human Intestinal Epithelial CaCo2/Bbe Cells by ctivating the p38 MAPK Pathway and Alleviates DSS-Induced Acute Colitis in C57BL/6 Mice (2010)
  • Author(s): Segawa S, Fujiya M, Ueno N, Kobayashi N, Ito K, Kohgo Y
  • Organizer: DDW 2010 (AGA)
  • Place of Presentation: New Orleans
  • Year and Date: 2010-05-04
• [Presentation] Heat-Killed Body of Lactobacillus brevis SBC8803 Contributes to Maintain Intestinal Homeostasis and Improve Intestinal Injury in a Murine Model of Colitis (2010)
  • Author(s): Ueno N, Fujiya M, Segawa S, Nata T, Inaba Y, Moriichi K, Tanabe H, Mizukami Y, Kobayashi N, Ito K, Kohgo Y
  • Organizer: DDW 2010 (AGA)
  • Place of Presentation: New Orleans
  • Year and Date: 2010-05-04
• [Book] Trypsin acts as an activating enzyme in gut innate immunity, Trypsin: Structure, Biosynthesis and Functions Trypsin: Structure, Biosynthesis and Functions, edited by Kirk Weaver and Clayton Kelly. Hauppauge (NY) (2012)
  • Author(s): Tanabe H, Fujiya M, Kohgo Y
  • Total Pages: 133-139
  • Publisher: Nova Science Publishers Inc
• [Patent(Industrial Property Rights)] 腸管保護剤 (2014)
  • Inventor(s): 高後 裕,藤谷幹浩,上野伸展,瀬川修一,小林直之
  • Industrial Property Rights Holder: 旭川医科大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特許第5526320号
  • Acquisition Date: 2014-04-15
• [Patent(Industrial Property Rights)] 消化器癌に対する抗腫瘍剤 (2013)
  • Inventor(s): 高後 裕、藤谷幹浩,岡本耕太郎
  • Industrial Property Rights Holder: 旭川医科大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特許第5190849号
  • Acquisition Date: 2013-02-08
• [Patent(Industrial Property Rights)] 腸管保護剤 (2011)
  • Inventor(s): 高後 裕,藤谷幹浩,上野伸展,瀬川修一,小林直之
  • Industrial Property Rights Holder: 旭川医科大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCT/JP2011/057689
  • Filing Date: 2011-03-28
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