2013 Fiscal Year Final Research Report
Cartilage repair using a scaffold free tissue engineered construct derived from multipotent stem cell
| Project/Area Number |
22390291
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
NAKAMURA Norimasa 大阪大学, 臨床医工学融合研究教育センター, 招へい教授 (50273719)
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| Co-Investigator(Kenkyū-buntansha) |
MYOUI Akira 大阪大学, 医学部附属病院, 准教授 (10263261)
FUJIE Hiromichi 首都大学東京, システムデザイン学部, 教授 (20199300)
HOSHI Kazuto 東京大学, 医学部附属病院, 特任准教授(移行) (30344451)
TERAMURA Takeshi 近畿大学, 医学部附属病院, 講師 (40460901)
FUKUDA Kanji 近畿大学, 医学部, 教授 (50201744)
YOSHIKAWA Hideki 大阪大学, 医学系研究科, 教授 (60191558)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | ES cell / iPS cell / stem cell / 軟骨再生 / Scaffold / 軟骨分化 / 骨分化 |
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| Research Abstract |
We had developed a scaffold-free tissue-engineered construct (TEC) derived from synovial mesenchymal stem cells(Sy-MSC,Sy-TEC). Sy-TEC promoted cartilage repair in a porcine injury model. However, repaired cartilage by Sy-TEC has limited morphology in animal model especially in superficial zone.Embryonic stem cell(ESC) is expected as a better cell source for cartilage repair. We have made TEC derived from ESC (ES-TEC) and performed chondrogenic differentiation in vitro. Expression level of chondrogenic genes were significantly higher compared to Sy-TEC and equal to native chondrocyte.
We have transplanted TEC/B-TCP complex to osteochondral defect of rabbit femoral groove, and then performed histological assessment. Repair tissue by Sy-TEC was like fibrocartilage but that by ES-TEC was hyaline cartilage like morphology at early stage following surgery. These results suggest the feasibility of ES-TEC in cartilage repair.We also succeeded in developing the TEC derived from iPS cells.
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[Journal Article] Generation of hyaline cartilaginous tissue from mouse adult dermal fibroblast culture by defined factors2011
Author(s)
Hiramatsu, K., Sasagawa, S., Outani, H., Nakagawa, K., Yoshikawa, H., Tsumaki, N
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Journal Title
J Clin Invest
Volume: 121
Pages: 640-657
Peer Reviewed
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[Journal Article] Identification of small molecular compounds and fabrication of its aqueous solution by laser-ablation, expanding primordial cartilage2011
Author(s)
Ikegami, D., Iwai, T., Ryo, S.I., Gu, N., Sugiyama, T., Oh, I., Yoshikawa, H., Tsumaki, N
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Journal Title
Osteoarthritis Cartilage
Volume: 19
Pages: 233-241
Peer Reviewed
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[Journal Article] The influence of skeletal maturity on allogenic synovial mesenchymal stem cell-based repair of cartilage in a large animal model2010
Author(s)
Shimomura K, Ando W, Tateishi K, Nansai R, Fujie H, Hart DA, Kohda H, Kita K, Kanamoto T, Mae T, Nakata K, Shino K, Yoshikawa H, Nakamura N
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Journal Title
Biomaterials
Volume: 31(31)
Pages: 8004-11
Peer Reviewed
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[Presentation] Osteochondral repair using a novel biphasic implant made of scaffold-free tissue engineered construct derived from synovial mesenchymal stem cells and hydroxyapatite-based artificial bone2012
Author(s)
Shimomura K, Moriguchi Y, Ando W, Nansai R, Susa T, Imade K, Mochizuki S, Fujie H, Kita K, Mae T, Nakata K, Yoshikawa H, Nakamura N
Organizer
International Cartilage Repair Society 2012
Place of Presentation
Montreal
Year and Date
2012-05-12
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[Presentation] Osteochondral repair using a novel biphasic implant made of scaffold-free tissue engineered construct derived from synovial mesenchymal stem cells and hydroxyapatite-based artificial bone2012
Author(s)
Shimomura K, Moriguchi Y, Ando W, Nansai R, Susa T, Imade K, Mochiduki S, Fujie H, Kita K, Mae T, Nakata K, Shino K, Yoshikawa H, Nakamura N
Organizer
Annual meeting of Orthopaedic Research Society 2012
Place of Presentation
San Francisco
Year and Date
2012-02-05
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