2012 Fiscal Year Final Research Report
Comprehensive risk analysis of bat-borne zoonosis with consideration to its biological features and environmental factors
| Project/Area Number |
22405039
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Applied veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KYUWA Shigeru 東京大学, 大学院・農学生命科学研究科, 教授 (30177943)
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| Co-Investigator(Kenkyū-buntansha) |
AKASHI Hiroomi 東京大学, 大学院・農学生命科学研究科, 教授 (10334327)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIKAWA Yasuhiro 東京大学, 大学院・農学生命科学研究科, 教授 (80109975)
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| Project Period (FY) |
2010 – 2012
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| Keywords | コウモリ / 翼手目 / レストンエボラウイルス / コウモリコロナウイルス / 抗体検査 / RT-PCR / 疫学 / 捕獲調査 |
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| Research Abstract |
We improved the method to detect viral RNA, combining classical RDV and size segregation. We developed an IgG-ELISA system to detect antibody to Reston ebolavirus (REBOV) glycoprotein. By IgG-ELISA to glycoproteins and nuclear proteins, we examined whether 141 bat sera in Luzon Island had antibodies to REBOV or not and found that a serum of R. amplexicaudatus was positive in the antibody responses to both viral proteins. In addition, positive responses against either REBOV-GP or -NP were also observed in 15 sera of the same bat species caught in and around Mindanao Island. We characterized bat xenobiotic metabolizing enzymes, determining full-size or almost full-size nucleotide sequences of CYP1A, CYP3B, CYP2D and UGT1A.
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[Journal Article] Analysis of the humoral immune responses among cynomolgus macaque naturally infected with Reston virus during the 1996 outbreak in the Philippines.2012
Author(s)
Taniguchi S, Sayama Y, Nagata N, Ikegami T, Miranda ME, Watanabe S, Iizuka I, Fukushi S, Mizutani T, Ishii YSaijo M, Akashi H, Yoshikawa Y, Kyuwa S, Morikawa S.
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Journal Title
DOI
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[Journal Article] Serologic evidence of nipah virus infection in bats, Vietnam.2012
Author(s)
Hasebe F, Thuy NT, Inoue S, Yu F, Kaku Y, Watanabe S, Akashi H, Dat DT, Mai le TQ, Morita K.
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Journal Title
Emerg Infect Dis.
Volume: 18
Pages: 536-537
DOI
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[Journal Article] Genomic and serological detection of bat coronavirus from bats in the Philippines.2012
Author(s)
Tsuda S, Watanabe S, Masangkay JS, Mizutani T, Alviola P, Ueda N, Iha K, Taniguchi S, Fujii H, Kato K, Horimoto T, Kyuwa S, Yoshikawa Y, Akashi H.
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Journal Title
Arch Virol.
Volume: 157
Pages: 2349-2355
DOI
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[Journal Article] Detection of bat coronaviruses from Miniopterus fuliginosus in Japan.2012
Author(s)
Shirato K, Maeda K, Tsuda S, Suzuki K, Watanabe S, Shimoda H, Ueda N, Iha K, Taniguchi S, Kyuwa S, Endoh D, Matsuyama S, Kurane I, Saijo M, Morikawa S, Yoshikawa Y, Akashi H, Mizutani T.
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Journal Title
Virus Genes.
Volume: Feb;44(1):40-4
DOI
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[Journal Article] Reston Ebolavirus antibodies in bats, the Philippines.2011
Author(s)
Taniguchi S, Watanabe S, Masangkay JS, Omatsu T, Ikegami T, Alviola P, Ueda N, Iha K, Fujii H, Ishii Y, Mizutani T, Fukushi S, Saijo M, Kurane I, Kyuwa S, Akashi H, Yoshikawa Y, Morikawa S.
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Journal Title
Emerg Infect Dis.
Volume: 17
Pages: 1559-1560
DOI
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[Journal Article] Bat coronaviruses and experimental infection of bats, the Philippines.2010
Author(s)
Watanabe S, Masangkay JS, Nagata N, Morikawa S, Mizutani T, Fukushi S, Alviola P, Omatsu T, Ueda N, Iha K, Taniguchi S, Fujii H, Tsuda S, Endoh M, Kato K, Tohya Y, Kyuwa S, Yoshikawa Y, Akashi H.
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Journal Title
Emerg Infect Dis.
Volume: 16
Pages: 1217-1223
DOI
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[Journal Article] Functional analysis of Rousettus aegyptiacus "signal transducer and activator of transcription 1"(STAT1).2010
Author(s)
Fujii H, Watanabe S, Yamane D, Ueda N, Iha K, Taniguchi S, Kato K, Tohya Y, Kyuwa S, Yoshikawa Y, Akashi H.
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Journal Title
Dev Comp Immunol.
Volume: 34
Pages: 598-602
DOI
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[Journal Article] Novel betaherpes-virus in bats.
Author(s)
Watanabe S, Maeda K, Suzuki K, Ueda N, Iha K, Taniguchi S, Shimoda H, Kato K, Yoshikawa Y, Morikawa S, Kurane I, Akashi H, Mizutani T.
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Journal Title
Emerg Infect Dis. 2010
Volume: 16
Pages: 986-988
DOI
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[Presentation] Analysis of the humoral immune responses among cynomolgus monkeys naturally infected with Reston ebolavirus during 1996 outbreak in the Philippines.2012
Author(s)
Satoshi Taniguchi, Yusuke Sayama, Noriyo Nagata, Tetsuro Ikegami, Mary E. Miranda, Shumpei Watanabe, Itoe Iizuka, Shuetsu Fukushi, Tetsuya Mizutani, Yoshiyuki Ishii, Masayuki Saijo, Hiroomi Akashi, Yasuhiro Yoshikawa, Shigeru Kyuwa, Shigeru Morikawa.
Organizer
The 9th JAPAN-CHINA International Conferenceof Virology
Place of Presentation
北海道大学フラテ会館、北海道
Year and Date
2012-06-12
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[Presentation] EPIDEMIOLOGICAL STUDY ON BAT CORONAVIRUS IN PHILIPPINES2011
Author(s)
Shumpei Tsuda, Shumpei Watanabe, Joseph S Masangkay, Phillip Alviola, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hikaru Fujii, Kentaro Kato, Taisuke Horimoto, Tetsuya Mizutani, Yumi Une, Shigeru Kyuwa, Yasuhiro Yoshikawa, Hiroomi Akashi
Organizer
International Union of Microbiological Societies 2011 Congress
Place of Presentation
Sapporo, Japan
Year and Date
2011-09-11
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[Presentation] ikawa, Shigeru Morikawa、THE DETECTION OF RESTON EBOLAVIRUS ANTIBODIES IN WILD BATS IN THE PHILIPPINES2011
Author(s)
Satoshi Taniguchi, Shumpei Watanabe, Koichiro Iha, Shuetsu Fukushi, Tetsuya Mizutani, Masayuki Saijo, Ichiro Kurane, Shigeru Kyuwa, Hiroomi Akashi, YasuhiroYosh
Organizer
International Union of Microbiological Societies 2011 Congress
Place of Presentation
Sapporo, Japan
Year and Date
2011-09-11
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[Presentation] ESTABLISHMENT OF SEROLOGICAL DIAGNOSIS OFARGENTINE HEMORRHAGIC FEVER USING RECOMBINANT ANTIGENS2011
Author(s)
Koichiro Iha, Mina Nakauchi-Hori, Satoshi Taniguchi, Shuetsu Fukushi, Tetsuya Mizutani, Momoko Ogata, Shigeru Kyuwa, Masayuki Saijo, Victor Romanowski, Delia A Enria, Shigeru Morikawa
Organizer
International Union of Microbiological Societies 2011 Congress
Place of Presentation
Sapporo, Japan
Year and Date
2011-09-11
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