Biological relevance of Coenzyme Q10 binding protein.-Study with prosaposin knockout animal and cell lines-

2012 Fiscal Year Final Research Report

• Project/Area Number: 22500681
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied health science
• Research Institution: Tokyo University of Technology
• Principal Investigator: YAMAMOTO Yorihiro 東京工科大学, 応用生物学部, 教授 (60134475)
• Co-Investigator(Kenkyū-buntansha): KASHIBA Misato 東京工科大学, 医療保健学部, 講師 (80338186)
• Co-Investigator(Renkei-kenkyūsha): YOSHIMURA Shinichi 東海大学, 医学部, 講師 (30230808)
• Project Period (FY): 2010 – 2012
• Keywords: コエンザイム Q10 / プロサポシン / サポシン / 脂質 / 脂質結合タンパク質 / ノッ クアウトマウス

Research Abstract

Coenzyme Q10 (CoQ10) is necessary for ATP biosynthesis. It also is an antioxidant. Saposin B (SapB) is a CoQ10 binding protein.Analysis using prosaposin (Psap, precursor of SapB) knockout mouse revealed that concentrations of exogenous CoQ10 in plasma and liver were significantly reduced in homo-type animal. Psap transfectant (Tf) and knockdown (KD) HepG2 cells are established. CoQ10 contents decreased as follows: Tf > parent > KD. These data imply that SapB and/orits precursor Psap is a modulator for CoQ10 contents.

Research Products

• [Journal Article] Carbon tetrachloride induced hepatic injury through the formation of oxidized diacylglycerol and its activation of the PKC/NF-κB pathway (2013)
  • Author(s): Toriumi K, Horikoshi Y, Henzan H, R. Osamura Y, Yamamoto Y, Nakamura N, Takekoshi S
  • Journal Title: Lab Invest Volume: 93 Pages: 218-229
  • DOI: doi:10.1038/labinvest.2012.145
  • Peer Reviewed
• [Journal Article] Increased Oxidative Stress and Coenzyme Q10 Deficiency in Juvenile Fibromyalgia: Amelioration of Hypercholesterolemia and Fatigue by Ubiquinol-10 (2013)
  • Author(s): Miyamae T, Seki M, Naga T, Uchino S, Asazuma H, Yoshida T, Iizuka Y, Kikuchi M, Imagawa T, Natsumeda Y, Yokota S, Yamamoto Y
  • Journal Title: Supplementation Redox Report Volume: 18 Pages: 12-19
  • DOI: doi:10.1179/1351000212Y.0000000036
  • Peer Reviewed
• [Journal Article] Hemodilution as a result of aggressive fluid resuscitation aggravates coagulopathy, but not survival, in a rat model of uncontrolled hemorrhagic shock (2013)
  • Author(s): Nishi K, Takasu A, Shinozaki H, Yamamoto Y, Sakamoto T
  • Journal Title: J Trauma Acute Care Surg, g Volume: 74 Pages: 808-12
  • DOI: doi:10.1097/TA.0b013e31827e1899
  • Peer Reviewed
• [Journal Article] Adcock IM Goto H Up-regulation of blood arachidonate (20:4) levels in patients with chronic obstructive pulmonary disease (2012)
  • Author(s): Wada H, Hagiwara S, Saitoh E, Ieki E, Yamamoto Y
  • Journal Title: Biomarkers Volume: 17 Pages: 520-523
  • DOI: doi:10.3109/1354750X.2012.692393
  • Peer Reviewed
• [Journal Article] Hypothermia reduces resuscitation fluid volumes required to maintain blood pressure in a rat hemorrhagic shock model (2012)
  • Author(s): Nishi K, Takasu A, Shibata M, Uchino S, Yamamoto Y, Sakamoto T
  • Journal Title: J Trauma Acute Care Surg Volume: 72 Pages: 130-135
  • DOI: doi:10.1097/TA.0b013e31821e61d8
  • Peer Reviewed
• [Presentation] コエンザイムQ10結合タンパク質プロサポシンはビタミンEも結合する (2012)
  • Author(s): 柿澤祐樹,山崎好裕,勇内山広樹,長谷川誠,森内寛,山本順寛
  • Organizer: 日本コエンザイムQ協会研究会発表場所
  • Place of Presentation: 東京都
  • Year and Date: 2012-01-24
• [Presentation] コエンザイムQ結合蛋白質プロサポシン発現量改変株の酸化ストレスに対する感受性 (2012)
  • Author(s): ジョン・チュンユン,長嶋康平,大泉美希子,鈴木優,森内寛,加柴美里,吉村眞一,山本順寛
  • Organizer: 日本コエンザイムQ協会研究会
  • Place of Presentation: 東京都
  • Year and Date: 2012-01-24
• [Presentation] ヒト・プロサポシン過剰発現マウスにおけるコエンザイムQとビタミンEの体内動態 (2012)
  • Author(s): 関学,浅利真司,長尾美好,山本順寛,吉村眞一
  • Organizer: 日本コエンザイムQ協会研究会
  • Place of Presentation: 東京都
  • Year and Date: 2012-01-24
• [Presentation] 母乳中のコエンザイムQ10とその結合タンパク質プロサポシン (2012)
  • Author(s): 宮内優樹,関学,宮前多佳子,藤田秀次郎,石田史彦,森内寛,加柴美里,横田俊平,山本順寛
  • Organizer: 日本コエンザイムQ協会研究会
  • Place of Presentation: 東京都
  • Year and Date: 2012-01-24
• [Presentation] プロサポシン遺伝子の改変で細胞内CoQ10の量とその酸化還元状態が変わる (2011)
  • Author(s): 加柴美里,大泉美希子,鈴木優,澤村佳美,寺嶋政之,長嶋康平,及川慎吾,ジョン・チュンユン,森内寛,吉村眞一,山本順寛
  • Organizer: 日本酸化ストレス学会関東支部会
  • Place of Presentation: 東京都
  • Year and Date: 2011-12-10
• [Presentation] Yamamoto YReduction of oxidative stress in successfully treated patients with community acquired pneumonia (CAP), as measured by redox status of coenzyme Q10 (%CoQ-10) (2011)
  • Author(s): Honda K, Wada H, Tamura M, Takata S, Nakamura M, Yasutake T, Kurai D, Ishii H, Goto H, Fujii K
  • Organizer: European Respiratory SocietyMeeting
  • Place of Presentation: Amsterdam, Nederland
  • Year and Date: 2011-09-30
• [Presentation] ヒト・プロサポシン過剰発現マウスにおけるCoQ含量とサポシンBの組織内分布 (2011)
  • Author(s): 吉村眞一,長尾美好,浅利真司,川合巧真,関学,内野晋也,加柴美里,山本順寛,大塚正人,猪子英俊
  • Organizer: 日本コエンザイムQ協会研究会
  • Place of Presentation: 東京都
  • Year and Date: 2011-01-28
• [Presentation] コエンザイムQ10結合タンパク質プロサポシンファミリーの生理的意義に関する考察 (2011)
  • Author(s): 山本順寛,加柴美里,吉村眞一
  • Organizer: 日本コエンザイムQ協会研究会
  • Place of Presentation: 東京都
  • Year and Date: 2011-01-28
• [Presentation] Saposin B and its precursor protein prosaposin play key roles in absorption and transfer of CoQ10 (2010)
  • Author(s): Yorihiro Yamamoto, Misato Kashiba, and Shinichi Yoshimura
  • Organizer: The 6th Conference of the InternationalCoenzyme 10 Association
  • Place of Presentation: Bedford Hotel, Brussels, Belgium
  • Year and Date: 20100527-30
• [Book] 機能性化粧品と薬剤デリバリー (2013)
  • Author(s): 山本順寛
  • Publisher: シーエムシー(印刷中)
• [Book] Free Radical Biology in Digestive Diseases. Front Gastrointest Res (2011)
  • Author(s): Yorihiro Yamamoto
  • Total Pages: 64-70
  • Publisher: Karger