2012 Fiscal Year Final Research Report
Synthesis and metal ion-sensing properties of FRET-based fluorescent chemosensors
| Project/Area Number |
22550068
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
KAWAKAMI Jun 弘前大学, 大学院・理工学研究科, 准教授 (60261426)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 金属イオン / 蛍光共鳴エネルギー移動 / 蛍光性化学センサー / 2-アミノトリプタンスリン / 超分子化学 |
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| Research Abstract |
FRET is defined as an excited-state energy interaction between two fluorophores in which excited donor energy is transferred to an acceptor part without any photon-emission. In this study, some new FRET-based fluorescent chemosensors for metal ions were synthesized. For example, excited at 325 nm corresponding to the absorption of pyrene unit of 2-aminotryptanthrin-pyrene derivative (T2NH-PnP), emission at 600 nm from 2-aminotryptanthrin unit is observed, indicating that intramolecular FRET occurs in T2NH-PnP (FRET-on). However, when Hg^2+ or Cu^2+ is added to a solution of T2NH-PnP, the fluorescence of 2-aminotryptanthrin is dramatically quenched whereas that of pyrene is revived (FRET-off).
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