2012 Fiscal Year Final Research Report
In vitro-synthesis of highly pure viral particles and their application to nanotechnology
| Project/Area Number |
22560774
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | University of Hyogo |
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Principal Investigator |
IMATAKA Hiroaki 兵庫県立大学, 大学院・工学研究科, 教授 (50201942)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ウイルス / ウイルス粒子 / 試験管内合成 / セルフリー / カプシド / ナノテクノロジー |
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| Research Abstract |
We established procedures through which RNA viruses, EMCV and HEV are synthesized in vitro. We synthesized EMCV in the human cell extract-derived in vitro protein expression system from DNA, and purified it for analysis with a transmission electron microscope. HEV capsid proteins were produced in E. coli and purified. The purified HEV proteins were incubated in vitro for the formation of HEV viral capsules. To apply these viral particles to drug delivery and bio-imaging, the amino acid sequences of the capsid proteins of EMCV and HEV were altered.
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