Delivery system of nucleic acid drug for protection of pollen allergy

2012 Fiscal Year Final Research Report

• Project/Area Number: 22560777
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: National Institute for Materials Science
• Principal Investigator: HANAGATA Nobutaka 独立行政法人物質・材料研究機構, ナノテクノロジー融合ステーション, ステーション長 (10302796)
• Project Period (FY): 2010 – 2012
• Keywords: 生物機能工学

Research Abstract

We constructed delivery system of CpG oligodeoxynucleotide (CpG ODN) that is one of the useful drugs for treatment of allergic diseases such as pollen allergy. For the delivery, silicon nanoparticles (Si NPs) with 3.4 nm in average diameter were synthesized. Different immune-mediator cytokines were induced by CpG ODN bound to Si NPs with different binding modes. Simultaneous application of CpG ODN bound to Si NPs with both binding modes induced higher immune response.

Research Products

• [Journal Article] Structure-dependent immunostimulatory effect of CpG oligodeoxynucleotides and their delivery system (2012)
  • Author(s): Hanagata, N
  • Journal Title: International Journal of Nanomedicine Volume: 7 Pages: 2181-2195
  • Peer Reviewed
• [Journal Article] Effect of molecular weight of polyethyleneimine on loading of CpG oligodeoxynucletotides onto flake-shell silica nanoparticles for enhanced TLR9-mediated induction of interferon-α (2012)
  • Author(s): Manoharan Y., Ji Q., Yamazaki T., Shanmugavel C., Chen S., Ganesan S., Hill JP., Ariga K., Hanagata N.
  • Journal Title: International Journal of Nanomedicine Volume: 7 Pages: 3625-3635
  • Peer Reviewed
• [Journal Article] Binding mode of CpG oligodeoxynucleotides to nanoparticles regulates bifurcated cytokine induction via Toll-like receptor 9 (2012)
  • Author(s): Chinnathambi S., Chen S., Ganesan S., Hanagata N
  • Journal Title: Scientific Reports Volume: 2 Pages: 534
  • Peer Reviewed
• [Journal Article] Identification of a boron nitride nanosphere-binding peptide for the intracellular delivery of CpG oligodeoxynucleotides (2012)
  • Author(s): Zhang H., Yamazaki T., Zhi C., Hanagata N
  • Journal Title: Nanoscale Volume: 4 Pages: 6343-6350
  • Peer Reviewed
• [Journal Article] T L R 9を介したC p G誘導サイトカインのナノ粒子によるスイッチング (2012)
  • Author(s): Hanagata N.
  • Journal Title: アレルギーの臨床 Volume: 32 Pages: 83-87
• [Journal Article] Design of mesoporous silica/CpG oligonucleaotides complexes for enhancement of delivery efficiency (2011)
  • Author(s): Zhu, Y., Meng, W., Li, X., Gao, H., Hanagata,N
  • Journal Title: The Journal of Physical Chemistry Volume: 115 Pages: 447-452
  • Peer Reviewed
• [Journal Article] BN nanospheres as CpG ODN carrier for activation of toll-like receptor 9 (2011)
  • Author(s): Zhi, C., Meng, W., Golberg, D., Tang, C., Hanagata, N.
  • Journal Title: Journal of Materials Chemistry Volume: 21 Pages: 5219-5222
  • Peer Reviewed
• [Journal Article] Dispersible shortened boron nitride nanotubes with improved molecular-loading capacity (2011)
  • Author(s): Zhi, C., Hanagata, N., Bando, Y., Golberg D.
  • Journal Title: Chemistry an Asian Journal Volume: 6 Pages: 2530-2535
  • Peer Reviewed
• [Journal Article] Hollow mesoporous silica/poly(L-lysine) particles for codelivery of drug and gene with enzyme-triggered release property (2011)
  • Author(s): Zhu, Y., Meng, W., Gao, H., Hanagata, N.
  • Journal Title: The Journal of Physical Chemistry C Volume: 115 Pages: 13630-13636
  • Peer Reviewed
• [Journal Article] Cytosine-phosphodiester-guanine oligodeoxynucleotide (CpG ODN)-capped hollow mesoporous silica particles for enzyme-triggered drug delivery (2011)
  • Author(s): Zhu, Y., Meng, W., Hanagata, N.
  • Journal Title: Dalton Transaction Volume: 40 Pages: 10203-10208
  • Peer Reviewed
• [Journal Article] Nuclease-resistant immunostimulatory phosphodiester CpG oligodeoxynucleotides as human Toll-like receptor 9 agonists (2011)
  • Author(s): Meng, W., Yamazaki, T., Nishida, Y., Hanagata, N
  • Journal Title: BMC Biotechnology Volume: 11 Pages: 88
  • Peer Reviewed
• [Presentation] Regulation of Toll-like Receptor 9 mediated Cytokine Profile by Binding Mode of CpG Oligodeoxynucleotides to Nanoparticles (2012)
  • Author(s): Hanagata, N
  • Organizer: 1st International Conference on Emerging Advanced Nanomaterials (ICEAN 2012)
  • Place of Presentation: Mercure Hotel, Brisbane, Australia
  • Year and Date: 20121022-25
• [Presentation] Preparation and characterization of silicon quantum dots for delivery of nucleic acid drugs. 4th International Conference on Advanced Nanomaterials (ANM2012) (2012)
  • Author(s): Shanmugavel, C., Ganesan S., Hanagata, N
  • Organizer: Indian Institute of Technology Madras (IIT Madras)
  • Place of Presentation: Chennai, India
  • Year and Date: 20121017-19
• [Presentation] Synthesis and characterization of positively charged silicon quantum dots for delivery of nucleic acid drugs (2012)
  • Author(s): Shanmugavel, C., Hanagata, N
  • Organizer: 第6回 ナノバイオメディカル学会大会
  • Place of Presentation: 独立行政法人産業技術総合研究所
  • Year and Date: 20120709-10
• [Presentation] Effect of interaction mode of CpG oligodeoxynucleotides with TLR9 on bifurcated cytokine induction (2012)
  • Author(s): Shanmugavel, C., Hanagata, N
  • Organizer: 第6回 ナノバイオメディカル学会大会
  • Place of Presentation: 独立行政法人産業技術総合研究所
  • Year and Date: 20120709-10
• [Presentation] Identification of a specific binding peptide to boron nitride nanospheres for delivery of CpG oligodeoxynucleotides (2012)
  • Author(s): Zhang, H., Yamazaki, T., i Zhi, C., Hanagata, N.
  • Organizer: 第6回 ナノバイオメディカル学会大会
  • Place of Presentation: 独立行政法人産業技術総合研究所
  • Year and Date: 20120709-10
• [Presentation] Response of human lung epithelial cells to the toxicity of zinc oxide nanoparticles (2012)
  • Author(s): Zhuang, F.,Hanagata, N.
  • Organizer: 第6回 ナノバイオメディカル学会大会
  • Place of Presentation: 独立行政法人産業技術総合研究所
  • Year and Date: 20120709-10
• [Presentation] Characterization of interleukin-6 induction by natural CpG oligodeoxynuxleotide (2012)
  • Author(s): Chechetka, S., Yamazaki, T., Hanagata, N.
  • Organizer: 第6回 ナノバイオメディカル学会大会
  • Place of Presentation: 独立行政法人産業技術総合研究所
  • Year and Date: 20120709-10
• [Presentation] N. New Nuclease-Resistant Immunostimulatory Phosphodiester-based Oligonucleotides for Immune Therapies (2012)
  • Author(s): Yamazaki, T.,Meng W., Hanagata
  • Organizer: The 9th World Biomaterials Congress (WBC)
  • Place of Presentation: Sichuan University,Sichuan China
  • Year and Date: 20120601-05
• [Presentation] Analysis of CpG ODNS binding of human toll like receptor 9 (2011)
  • Author(s): Suwarti, Yamazaki, T., Hanagata, N.
  • Organizer: Infection and Immunity (Cold Spring Harbor Asia)
  • Place of Presentation: Suzhou Dushu Lake Conference Center, Shuzou, China
  • Year and Date: 20110908-12
• [Presentation] Novel CpG phosphodiester oligodeoxynucleotides as human toll-like receptor 9 agonists (2011)
  • Author(s): Yamazaki, T., Meng, W., Hanagata, N.
  • Organizer: Infection and Immunity (Cold Spring Harbor Asia)
  • Place of Presentation: Suzhou Dushu Lake Conference Center, Shuzou, China
  • Year and Date: 20110908-12
• [Presentation] Design of mesoporous silica/CpG ODN complexes to enhance delivery efficiency (2011)
  • Author(s): Zhu, Y., Hanagata, N.
  • Organizer: International Conference on Materials for Advanced technologies
  • Place of Presentation: Suntec Singapore Hotel, Singapore
  • Year and Date: 20110626-0701
• [Presentation] Novel CpG oligodeoxynucleotide and its delivery system (2011)
  • Author(s): Yamazaki, Y., Meng, W., Zhi, C., Zhu, Y., Hanagata, N.
  • Organizer: 9th annual Bio Nanotech Conference
  • Place of Presentation: Boston, US
  • Year and Date: 20110613-16
• [Presentation] Enhancement of the theraqeutic efficiency of a human toll-lilke receptor 9 agonist via bio-and nano-technology (2011)
  • Author(s): Yamazaki, T. Hanagata, N.
  • Organizer: International Workshop on Biomedical Sciences and Technologies
  • Place of Presentation: Anna University, Chennai, India
  • Year and Date: 20110302-04
• [Presentation] Construction of nuclear-resistant phosphodiester CpG oligonucleotides for pollinosis therapy (2011)
  • Author(s): Meng, W., Yamazaki, T., Hanagata, N.
  • Organizer: ナノ・バイオメディカル学会
  • Place of Presentation: 名古屋大学野依記念学術交流会館
  • Year and Date: 20110221-22
• [Presentation] Molecular organization at the live cell-biomembrane interface (2011)
  • Author(s): Zhang, L., Kaizuka, Y., Hanagata, N.
  • Organizer: ナノ・バイオメディカル学会
  • Place of Presentation: 名古屋大学野依記念学術交流会館
  • Year and Date: 20110221-22
• [Presentation] Fe3O4@Sio2 hollow mesoporous spheres as potential carrier for anticancer drug delivery (2011)
  • Author(s): Zhu, Y., Hanagata, N.
  • Organizer: ナノ・バイオメディカル学会
  • Place of Presentation: 名古屋大学野依記念学術交流会館
  • Year and Date: 20110221-22
• [Presentation] BN nanospheres as CpG ODN carrier for activation of toll-like receptor 9 (2011)
  • Author(s): Zhi, C., Meng, W., Yamazaki, T., Bando, Y., Golberg, D., Tang, C., Hanagata, N.
  • Organizer: ナノ・バイオメディカル学会
  • Place of Presentation: 名古屋大学野依記念学術交流会館
  • Year and Date: 20110221-22
• [Presentation] Water soluble silicon quantum dots as fluorescent probes for bio-applications (2011)
  • Author(s): Chinnathambi S., Krishnan, V., Ariga, K., Hanagata, N.
  • Organizer: The Symposium on Micro/Nano Aspects of Biomaterials
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-07-19
• [Presentation] C-terminal ligand binding analysis of human Toll-like receptor 9 (2011)
  • Author(s): Suwarti, Yamazaki, T., Hanagata, N.
  • Organizer: The Symposium on Micro/Nano Aspects of Biomaterials
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-07-19
• [Presentation] Identification of Boron Nitride Nanospheres Binding Peptides (2011)
  • Author(s): Zhang, H., Yamazaki, T., Zhi, C., Hanagata, N.
  • Organizer: The Symposium on Micro/Nano Aspects of Biomaterials
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-07-19
• [Presentation] Novel Phosphodiester CpG Oligodeoxynucleotides with High Stimulatory Effects on Human Toll-like Receptor 9 (2011)
  • Author(s): Meng, W., Yamazaki, T., Hanagata, N.
  • Organizer: The Symposium on Micro/Nano Aspects of Biomaterials
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-07-19
• [Patent(Industrial Property Rights)] 免疫刺激オリゴヌクレオチドおよび前記免疫刺激オリゴヌクレオチドを含む治療剤 (2011)
  • Inventor(s): 山崎智彦、花方信孝
  • Industrial Property Rights Holder: 独立行政法人物質・材料研究機構
  • Industrial Property Number: 特願2011-070049
  • Filing Date: 2011-03-28