2012 Fiscal Year Final Research Report

Re-evaluation of microbial methane stable carbon isotope frac-tionation under high hydrostatic pressure

Research Project

Project/Area Number	22560811
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Earth system and resources enginnering
Research Institution	Japan Agency for Marine-Earth Science and Technology
Principal Investigator	TASUMI Eiji 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 技術研究副主任 (50553228)
Co-Investigator(Kenkyū-buntansha)	IMACHI Hiroyuki 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (20361933) TAKAI Ken 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, プログラムディレクター (80359166) KAWAGUCHI Shinsuke 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 研究員 (50553088)
Project Period (FY)	2010 – 2012
Keywords	メタン
Research Abstract	Methanosarcina sp. NT-MS1 that had been isolated from Nankai Trough subseafloor sediment using batch-culture was cultured under high pressure conditions. Its optimum pressure for growth was 15 MPa, and it showed positive growth with methanogenesis at its habitat pressure (25 MPa). Takai et al. reported that the hyperthermophilic methanogen, Methanopyrus kandleri strain 116, generates “isotopically heavy” methane under high pressure conditions. But, strain NT-MS1 generated “isotopically light (Δ~<13>C-CH_4 = -81～-63‰)” methane at 5 to 35 Mpa.

(4 results)