2012 Fiscal Year Final Research Report
Modification of chloroplast proteins with reactive aldehydes: its implication in the plant response to oxidative stress
| Project/Area Number |
22570044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
MANO Junichi 山口大学, 大学研究推進機構, 教授 (50243100)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUI Kenjhi 山口大学, 大学院・医学系研究科, 教授 (90199729)
TANAKA Kiyoshi 鳥取大学, 農学部, 教授 (50124350)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 光合成 / 酸化ストレス / 環境応答 / アクロレイン / タンパク質修飾 |
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| Research Abstract |
We obtained following results about the action of lipid peroxide-derived reactive aldehydes (RAL) in plant responses to environmental stress:
(1) The RAL species responsible for the aluminum-induced oxidative injury in roots and those for the strong-light-induced photooxidative injury in leaves identified.
(2) Light-dependent formation of organic peroxides on the donor side of photosystem II that lacked the water-oxidizing complex was proven.
(3) In NaCl-stressed plants, modification of proteins with RAL were increased. The modified targets were identified.
(4) The target amino acid(s) in phosphoribulokinase that have primary importance in the sensitivity to acrolein was not the redox-regulated Cys.
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[Journal Article] Modulation of activity and inhibitor sensitivity of rabbit aldose reductase-like protein (AKR1B19) by oxidized glutathione and SH-reagents2013
Author(s)
Endo S., Fujimoto, A., Kumada, S., Matsunaga, T., Ohno S., Mano, J., Tajima, K., El-Kabbani, O., Hara, A
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Journal Title
Chem. Biol. Interact
Volume: 202
Pages: 146-152
Peer Reviewed
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[Journal Article] Characterization of rabbit aldose reductase-like protein with 3 -hydroxysteroid dehydrogenase activit2012
Author(s)
Endo, S., Matsunaga, T., Kumada, S., Fujimoto, A., Ohno, S., El-Kabbani, O., Hu, D., Toyooka, N., Mano, J., Tajima, K. and Hara, A
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Journal Title
Arch. Biochem. Biophys
Volume: 527
Pages: 23-30
Peer Reviewed
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[Journal Article] Characterization of raspberry ketone/zingerone synthase, catalyzing the alpha,beta-hydrogenation of phenylbutanones in raspberry fruits2011
Author(s)
Koeduka, T., Watanabe, B., Suzuki, S., Hiratake, J., Mano, J. and Yazaki, K
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 412
Pages: 104-108
Peer Reviewed
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[Journal Article] Accumulation of lipid peroxide-derived, toxic , -unsaturated aldehydes (E)-2-pentenal, acrolein and (E)-2-hexenal in leaves under photoinhibitory illumination2010
Author(s)
Mano, J., Tokushige, K., Mizoguchi, H., Fujii, H. and Khorobrykh, S
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Journal Title
Plant Biotechnol
Volume: 27
Pages: 193-197
Peer Reviewed
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