Function of new elastic protein

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570153
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Chiba University
• Principal Investigator: KIMURA Sumiko 千葉大学, 大学院・理学研究科, 教授 (50093232)
• Project Period (FY): 2010 – 2012
• Keywords: 高分子量タンパク質 / 無脊椎動物 / 生体分子 / 超遠心分離法 / 弾性タンパク質

Research Abstract

Connectin is an elastic protein in vertebrate striated muscle. Invertebrates also possess various connectin-like proteins, one of which is a connectin-like 4000 kDa protein that is present in the obliquely striated muscle of Annelida. We have determined the nucleotide sequence of the gene which encodes a connectin-like 4000 kDa protein in the body wall muscle of Neanthes sp. We discovered a sequence in which a unit of 47 amino acids was repeated 11 times. After preparing the recombinant protein, we performed analytical ultracentrifugation and measured CD spectrum to estimate its secondary structure content. The sedimentation velocity experiment revealed that the protein had an extremely asymmetric shape, and 48% of the whole structure assumed beta-structure. It was thus demonstrated that this protein could cover a longer distance than other ordinary proteins with the same size.
The fact that the connectin-like 4000 kDa protein of the obliquely striated muscle of Annelida has a distinct structure with the 47-amino acid repeat sequence may be related to the resting length of a sarcomere in the Annelida obliquely striated muscle, which is about twice as long as that in the vertebrate striated muscle.

Research Products

• [Journal Article] Actin capping proteins, CapZ (β-actinin) and tropomodulin in amphioxus striated muscle (2012)
  • Author(s): Bao, Y., Kake, T., Hanashima, A., Nomiya, U., Kubokawa, K. and Kimura, S.
  • Journal Title: Gene Volume: Vol. 510 (1) Pages: 78-86
  • DOI: DOI:10.1016/j.gene.2012.07.081
  • Peer Reviewed
• [Journal Article] Tropomodulins: Pointed-end capping proteins that regulate actin filament architecture in diverse cell types (2012)
  • Author(s): Yamashiro, S., Gokhin, S.D., Kimura, S., Nowak, B.R., Velia M. Fowler, M.V.
  • Journal Title: Cytoskeleton (Hoboken, N.J.) Volume: Vol. 69 (6) Pages: 337-370
  • DOI: DOI:10.1002/cm.21031
  • Peer Reviewed
• [Journal Article] Genomic- and protein-based approaches for connectin (titin) identification in the ascidian Ciona intestinalis (2012)
  • Author(s): Hanashima, A., Ogasawara, M., Nomiya, Y., Sasaki, T., Bao, Y., Kimura, S.
  • Journal Title: Methods Volume: Vol. 56(1) Pages: 18-24
  • DOI: DOI:10.1016/j.ymeth.2011.12.010
  • Peer Reviewed
• [Journal Article] Amphioxus connectin exhibits merged structure as invertebrate connectin in I-band region and vertebrate connectin in A-band region (2011)
  • Author(s): Ohtsuka, S., Hanashima, A., Kubokawa, K., Bao, Y., Tando, Y., Kohmaru, J., Nakaya, H., Maruyama, K. and Kimura, S.
  • Journal Title: J. Mol. Biol Volume: Vol. 409 (3) Pages: 415-426
  • DOI: DOI:10.1016/j.jmb.2011.04.010
  • Peer Reviewed
• [Journal Article] Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin (2010)
  • Author(s): Chitose, R., Watanabe, A., Asano, M., Hanashima, A., Sasano, K., Bao, Y.,Maruyama, K. and Kimura, S.
  • Journal Title: J. Biomed. Biotechnol Pages: 108495
  • DOI: DOI:10.1155/2010/108495
  • Peer Reviewed
• [Journal Article] Protruding masticatory (superfast) myosin heads from poorly aligned thick filaments of dog jaw muscle revealed by X-ray diffraction (2010)
  • Author(s): Yamaguchi, M., Takemori, S., Kimura, M., Tanishima, Y., Nakayoshi, T., Kimura, S., Ohno, T., Yagi, N., Hoh, F.Y. J., Umazume, Y.
  • Journal Title: J. Biochem Volume: Vol. 147 (1) Pages: 53-61
  • DOI: DOI:10.1093/jb/mvp143
  • Peer Reviewed
• [Journal Article] Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation (2010)
  • Author(s): Takano, K., Watanabe-Takano, H., Suetsugu, S., Kurita, S., Tsujita, K., Kimura, S., Karatsu, T., Takenawa, T., Endo, T.
  • Journal Title: Science Volume: Vol. 330 Pages: 1536-1540
  • DOI: DOI:10.1126/science.1197767
  • Peer Reviewed
• [Presentation] Sequential analysis of Planarian connectin-like protein (2012)
  • Author(s): Sasano, K., Kurasawa, M., Hanashima, A., Bao, Y., Nakauchi, Y., Kimura, S.
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-22
• [Presentation] 47 amino acids repeat of connectin-like 4000K-protein in obliquely striated muscle of Annelida (2012)
  • Author(s): Nomiya, Y., Kanamaru, S., Arisaka, F., Kimura, S.
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-22
• [Presentation] Connectin-like protein in molluscan striated muscle (2012)
  • Author(s): Bao, Y., Hanashima, A., Sodeyama, F., Kimura, S.
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-22
• [Presentation] I-コネクチンの弾性繰り返し領域の構造解析 (2012)
  • Author(s): 中谷浩之、金丸周司、有坂文雄、木村澄子
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] ウニのコネクチン様タンパク質 (2012)
  • Author(s): 佐々木智子、松浦哲、花島章、野宮唯、木村澄子
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] 刺胞動物のコネクチン様タンパク質 (2012)
  • Author(s): 春山綾菜、花島章、木村澄子
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] 環形動物ゴカイ斜紋筋のコネクチン様タンパク質 (2012)
  • Author(s): 野宮唯、木村澄子
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] 脊椎動物のゲノム中におけるコネクチン遺伝子数の変遷 (2012)
  • Author(s): 花島章、木村澄子
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] ホヤのコネクチン様タンパク質 (2011)
  • Author(s): 佐々木智子、野宮唯、花島章、包玉龍、小笠原道生、木村澄子
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] 環形動物ゴカイ斜紋筋の4000Kタンパク質 (2011)
  • Author(s): 野宮唯、木村澄子
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] カイメン、ヒドラのコネクチン様タンパク質 (2011)
  • Author(s): 日高暢也、浜岡知哉、花島章、木村澄子
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] セイヨウカサガイ平滑筋のコネクチン様タンパク質 (2011)
  • Author(s): 包玉龍、花島章、袖山文彰、木村澄子
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] 扁形動物ナミウズムシDugesia japonicaのコネクチン様タンパク質 (2011)
  • Author(s): 佐々野耕平、花島章、包玉龍、中内祐二、木村澄子
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] Amphioxus striated muscle has beta-actinin and tropomodulin (2010)
  • Author(s): Bao, Y., Kake, T., Hanashima, A., Kubokawa, K., Tando, Y., Kimura, S.
  • Organizer: The 9th International Conference on Bioinformatics (InCoB'10)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-09-27
• [Presentation] The connectin-like protein gene of amphioxus striated muscle (2010)
  • Author(s): Ohtsuka, S., Hanashima, A., Kubokawa, K., Tando, Y., Kimura, S.
  • Organizer: The 9th International Conference on Bioinformatics (InCoB'10)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-09-27
• [Presentation] ウニコネクチン様蛋白質のドメイン構造 (2010)
  • Author(s): 松浦哲、花島章、包玉龍、木村澄子
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-25
• [Presentation] ホタテ貝のコネクチン様タンパク質 (2010)
  • Author(s): 包玉龍、木村澄子
  • Organizer: ホタテ貝のコネクチン様タンパク質
  • Place of Presentation: 東京
  • Year and Date: 2010-09-25
• [Presentation] Searching for connectin-like proteins in Platyhelminthes (2010)
  • Author(s): Sasano, K., Hanashima, A., Nakauchi, Y., Kimura, S.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-25
• [Presentation] Binding region of I-Connectin and Projectin in Crayfish Claw Closer Muscle (2010)
  • Author(s): Nakaya, H., Hiura, K., Kimura, S.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-20
• [Presentation] 筋肉の弾性タンパク質コネクチンのタンデムリピート (2010)
  • Author(s): 木村澄子
  • Organizer: 蛋白質科学会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-18