2012 Fiscal Year Final Research Report
Clarification of hemoglobin evolution process by reverse molecular evolution
| Project/Area Number |
22570217
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Hosei University |
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Principal Investigator |
IMAI Kiyohiro 法政大学, 生命機能学部, 教授 (50028528)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUO Takatoshi 法政大学, マイクロ・ナノテクノロジー, 研究員 (00500737)
NAKAGAWA Taro 長浜バイオ大学, バイオサイエンス学部, 助手 (30401889)
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| Co-Investigator(Renkei-kenkyūsha) |
NAGAI Masako (60019578)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ヘモグロビン / 分子進化 / 祖先型蛋白質 / 硬骨魚類 / 円口類 |
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| Research Abstract |
This study intends to learn the principle of protein molecule design which was adopted by nature to acquire high-order functions for ancestral hemoglobins of vertebrates in the course of molecular evolution. We designed and synthesized the hemoglobin genes for ancestors of teleost and cyclostomata and expressed them in E. coli, obtaining the ancestral hemoglobins. The teleost ancestral hemoglobins showed a strong Bohr effect which assumes a Root effect and week cooperativity, indicating that they have characteristics of teleost. The cyclostomata ancestral hemoglobins showed a small Bohr effect and week cooperativity in contrast with natural lamprey hemoglobin without those effects.
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[Journal Article] Near-UVcircular dichroism and UV resonance Ramanspectra of individual tryptophan residues inhuman hemoglobin and their changes uponthe quaternary st2012
Author(s)
Nagai, M, Nagatomo, S, Nagai, Y, Ohkubo,K, Imai, K, and Kitagawa, T.
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Journal Title
Biochemistry
Volume: 51
Pages: 5932-5941
Peer Reviewed
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[Journal Article] Relationship between oxygen affinity and autoxodation of myoglobin.2012
Author(s)
Shibata, T, Matsumoto, D;, Nishimura, R, Tai, H, Matsuoka, A, Nagao, S, Matsuo, T, Hirata, S, Imai, K, Neya, S, Suzuki, A. and Yamamoto, T.
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Journal Title
Inorg. Chem
Volume: 51
Pages: 11955-11960
Peer Reviewed
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