2012 Fiscal Year Final Research Report
Studies on the relevance of cell-to-cell movement and replication mechanisms of Dianthovirus
| Project/Area Number |
22580047
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Kyoto University |
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Principal Investigator |
KAIDOU Masanori 京都大学, 大学院・農学研究科, 助教 (20314247)
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| Co-Investigator(Renkei-kenkyūsha) |
TANIGUCHI Hisaaki 徳島大学, 疾患酵素学研究センター, 教授 (10257636)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 植物ウイルス / 細胞間移行 / 複製酵素複合体 / 小胞体膜 / 宿主因子 |
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| Research Abstract |
Using tandem affinity purification and mass spectrometry, a lot of host factor candidates that interact with the movement protein (MP) of Red clover necrotic mosaic virus (RCNMV) were identified from Nicotiana benthamianaplants. Among these, full-length cDNA of Glyceraldehyde 3-phosphate dehydrogenase（GAPDH-A）was cloned. GAPDH-Awas shown to be the host factor that is involved in the cell-to-cell movement of RCNMV. From the detailed study of GAPDH-A intracellular localization, I have revealed that GAPDH-Apositively regulates the colocalization of the MP and the replication complex to support efficientcell-to-cell movement of the virus.
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