Elucidation of the mechanisms for fruiting body formation founded on light signaltransduction

2012 Fiscal Year Final Research Report

• Project/Area Number: 22580097
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: MIYAZAKI Yasumasa 独立行政法人森林総合研究所, きのこ・微生物研究領域, 主任研究員 (40343800)
• Co-Investigator(Kenkyū-buntansha): KANEKO Shinya 東京工業大学, 大学院生命理工学研究科, 助教 (10399694)
• Project Period (FY): 2010 – 2012
• Keywords: きのこ / 光受容体 / 形態変化

Research Abstract

Fruiting body formation of mushroom requires light. The Le.cry gene in L. edodes encoded a cryptochrome photo receptor first isolated from basidiomycetes. The expression product, Le.CRY, was suggested to be involved in fruiting body formation via perception of blue light. As a result of proteome analysis, there existed metabolisms and signal transduction pathways involved in fruiting body formation, which were mediated by protein phosphorylation and glycosylation. Photo-responsive genes were comprehensively identified by transcriptome analysis.

Research Products

• [Journal Article] Differentially expressed genes under simulated microgravity in fruiting bodies of the fungus Pleurotus ostreatus (2010)
  • Author(s): Yasumasa Miyazaki, Masahide Sunagawa, Akira Higashibata, Noriaki Ishioka, Katsuhikot Babasaki, Takashi Yamazaki.
  • Journal Title: FEMS Microbiology Letters Volume: 307 Pages: 72-79
  • Peer Reviewed
• [Presentation] A DASH-type cryptochrome gene from Lentinula edodes (2011)
  • Author(s): Hiroaki Sano, Shinya Kaneko, Masaya Nakamura, Yasumasa Miyazaki
  • Organizer: The 7th International Conference on Mushroom Biology and Mushroom Products
  • Place of Presentation: Arcachon, France
  • Year and Date: 2011-10-06
• [Presentation] Light-stimulative effects on the cultivation of edible mushrooms by using blue LED (2011)
  • Author(s): Yasumasa Miyazaki, Kazuhiko Masuno, Masanori Abe, Hajime Nishizawa, Tetsuo Matsumoto, Sachio Kunitomo, Haruo Sakata, Kimiyoshi Nakamura, Tomoyuki Koyama, Masami Ito, Hiroshi Kazama, Dai Suzuki, Yasushi Obatake, Hiroaki Sano, Masaya Nakamura, Kazuhiro Miyazaki, Yuichi Sakamoto, Shinya Kaneko, Takashi Kamada
  • Organizer: Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products
  • Place of Presentation: Arcachon, France
  • Year and Date: 2011-10-06
• [Presentation] シイタケ子実体形成過程における発現タンパク質の解析 (2011)
  • Author(s): 佐野広明、中沢威人、金子真也、東端晃、石岡憲昭、角田光利、鎌田堯、宮崎安将
  • Organizer: 日本農芸化学会2011 年度大会
  • Place of Presentation: 京都女子大学(京都府京都市)
  • Year and Date: 2011-03-26
• [Presentation] Identification of a cryptochrome gene homolog in the basidiomycetous mushroom Lentinula edodes (2010)
  • Author(s): Hiroaki Sano, Shinya Kaneko, Masahide Sunagawa, Kazuhiro Miyazaki, Mitsutoshi Tsunoda, Masaya Nakamura, Yasumasa Miyazaki
  • Organizer: The International Chemical Congress of Pacific Basin Societies (Pacfichem2010)
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-17
• [Presentation] Whole protein analysis by 2-D PAGE during fruiting body formation in Lentinula edodes (2010)
  • Author(s): Hiroaki Sano, Shinya Kaneko, Akira Higashibata, Noriaki Ishioka, Masahide Sunagawa, Yasumasa Miyazaki
  • Organizer: The 9th International Mycological Congress (IMC9)
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2010-08-04
• [Book] 森林総合研究所平成24年版研究成果選集 (2012)
  • Author(s): 宮崎安将、宮崎和弘、中村雅哉、佐野広明、金子真也、鎌田堯、坂本裕一、増野和彦、古 川仁、阿部正範、西澤元、中村公義、小山智 行、風間宏、鈴木大、國友幸夫、阪田春生、小畠靖
  • Total Pages: 62-63
  • Publisher: LED 照明を利用したきのこ栽培技術の開発
• [Book] 社団法人農業電化協会 (2010)
  • Author(s): 宮崎安将
  • Total Pages: 30-34
  • Publisher: 人工光源の農林水産分野への応用
• [Remarks] シイタケゲノム・データベース:(独)森林総合研究所遺伝子データベースForestGEN
  • URL: http://forestgen.ffpri.affrc.go.jp/ja/info_le.html