Novel cell signaling by different Gb3-binding marine lectins.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22580226
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Yokohama City University
• Principal Investigator: OZEKI Yasuhiro 横浜市立大学, 大学院・生命ナノシステム科学研究科, 教授 (70275022)
• Co-Investigator(Kenkyū-buntansha): YASUMITSU Hidetaro (10182346)
• Project Period (FY): 2010 – 2012
• Keywords: レクチン / MytiLec / 一次構造 / グロボトリオース / ABCトランスポーター / SUEL/ラムノース結合レクチンファミリー

Research Abstract

A17kDa α-galactoside-binding lectin,MytiLec purified from the Mediterranean mussel was found to have a novel triple-tandem repeat structure. It recognized globotriose (Gb3)and appeared strong cell static activity againstGb3 expressing lymphoma Raji cells. Another α-galactoside-binding lectin, SAL,a member of SUEL/rhamnose-binding lectin family isolated from catfish eggdid not influence the growth of Raji cells, though it down-regulated the expression levels of the multidrug-resistanttransporter gene. Combining these results, it became clear that even lectins having same oligosaccharide binding specificity, can transmit different signals to cells according to the variations of their multivalency and dissociation constants. This new finding will be usefulto study the cellular functions of these lectins to regulate cell proliferation.

Research Products

• [Journal Article] bubble mixing (FBM) culture of E. coli: a highly cost-effective middle scale-size culture system (2013)
  • Author(s): Yasumitsu H, Tajima H, Isobe M, Kutsna S, Kawsar SMA, Fujii Y, Kanaly RA, Ozeki Y, Yokota E
  • Journal Title: Protein Pept. Lett Volume: 20 Pages: 213-217
  • DOI: DOI: 10.2174/0929866511320020014
• [Journal Article] Domain composition of rhamnose-binding lectin from shishamo smelt eggs and its a carbohydrate-binding profile. (2013)
  • Author(s): Hosono M, Sugawara S, Tatsuta T, Hikita TKominami J, Nakamura-Tsuruta S, Hirabayashi J, Kawsar SMA, Ozeki Y, Hakomori-S-I, Nitta K
  • Journal Title: Fish Physiol Biochem
  • DOI: DOI:10.1007/s10695-013-9814-6
• [Journal Article] MRP1 expressed on Burkitt's lymphoma cells was depleted by catfish egg lectin through Gb3-glycosphingolipid and enhanced cytotoxic effect of drugs. (2012)
  • Author(s): Fujii Y, Sugawara S, Araki D, Kawano T, Tatsuta T, Takahashi K, Kawsar SMA, Matsumoto R, Kanaly RA, Yasumitsu H, Ozeki Y, Hosono M, Miyagi T, Hakomori S-I, Takayanagi Y, Nitta K
  • Journal Title: Protein J Volume: 31 Pages: 15-26
  • DOI: DOI:10.1007/s10930-011-9369-2
• [Journal Article] Cytotoxic and glycan-binding properties of an 18 kDa lectin isolated from themarine sponge Halichondria okadai (2012)
  • Author(s): Matsumoto R, Fujii Y, Kawsar SMA, Kanaly RA, Yasumitsu H, Koide Y, Hasan I, Ogawa Y, Im CH, Sugawara S, Hosono M, Nitta K, Hamako J, Matsui T, Ozeki Y
  • Journal Title: Toxins Volume: 4 Pages: 323-338
  • DOI: DOI:10.3390/toxins4050323
• [Journal Article] Identification and recombinant analysis of botrocetin-2, a snake venom cofactor for von Willebrand factor-induced platelet agglutination (2012)
  • Author(s): Yamamoto-Suzuki Y, Sakurai Y, Fujimura Y, Matsumoto M, Hamako J, Kokubo T, Kitagawa H, Kawsar SMA, Fujii Y, Ozeki Y, Matsushita F, Matsui T
  • Journal Title: Biochemistry Volume: 51 Pages: 5329-5338
  • DOI: DOI:10.1021/bi300442c
• [Journal Article] A lectin from the mussel Mytilus galloprovincialishas a highly novel primary structure and induces glycan-mediated cytotoxicity of globotriaosylceramide-expressing lymphoma cells (2012)
  • Author(s): Fujii Y, Dohmae N, Kawsar SMA, Matsumoto R, Hasan I, Koide Y, Kanaly RA, Yasumitsu H, Ogawa Y, Sugawara S, Hosono M, Nitta K, Hamako J, Matsui T, Ozeki Y
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 44772-44783
  • DOI: DOI:10.1074/jbc.M112.418012
• [Journal Article] Cytotoxicity and glycanbinding profile of a D-galactose-binding lectin from the eggs of a Japanese sea hare (Aplysia kurodai) (2011)
  • Author(s): Kawsar SMA, Matsumoto R, Fujii Y, Matsuoka H, Masuda N, Chihiro I, Yasumitsu H, Kanaly RA, Sugawara S, Hosono M, Nitta K, Ishizaki N, Dogasaki C, Hamako J, Matsui T, Ozeki Y
  • Journal Title: Protein J Volume: 30 Pages: 509-519
  • DOI: DOI:10.1007/s10930-011-9356-7
• [Journal Article] D-galactoside-binding lectin purified from coronate moon turban, Turbo(Lunella) coreensiswith a unique amino acid sequence and the ability to recognize lacto-series glycosphingolipids (2011)
  • Author(s): Fujii Y, Matsumoto R, Kawsar SMA, Yasumitsu H, Ishizuka N, Dogasaki C, Hosono M, Nitta K, Hamako J, Matsui T, Ozeki Y
  • Journal Title: Comp Biochem. Physiol. B Mol. Biol Volume: 158 Pages: 30-37
  • DOI: DOI:10.1016/j.cbpb.2010.09.002
• [Journal Article] Antiproliferative effects of galectin-1 from Rana catesbeianaeggs on human leukemia cells and its binding proteins in human cells (2011)
  • Author(s): Yasumitsu H, Mochida K, Yasuda C, Isobe M, Kawsar SMA, Fujii Y, Matsumoto R, Kanaly RA, Ozeki Y
  • Journal Title: In Vitro Cell Dev Biol Anim Volume: 47 (10) Pages: 728-734
  • DOI: DOI:10.1007/s11626-011-9462-z
• [Journal Article] Glycomics of a novel type-2 N-acetyllactosamine-specific lectin purified from the feather star (Oxycomantus japonicus) Pelmatozoa (class Crinoidea) (2011)
  • Author(s): Matsumoto R, Shibata FT, Kohtsuka H, Sekifuji M, Sugii N, Nakajima H, Kojima N, Fujii Y, Kawsar SMA, Yasumitsu H, Ozeki Y
  • Journal Title: Comp. Biochem. Physiol. B Mol. Biol Volume: 158 Pages: 266-273
  • DOI: DOI:10.1016/j.cbpb.2010.12.004
• [Journal Article] Toxicity assessment of a -galactoside-binding lectin (galectin-1) from bullfrog Rana catesbeianaunfertilized eggs (2011)
  • Author(s): Kawsar SMA, Aftabuddin S, Mostafa G, Yasumitsu H, Ozeki Y
  • Journal Title: Acta Pharma. Sci Volume: 53 Pages: 107-115
• [Journal Article] CGP stain: An inexpensive, odorless, rapid, sensitive, and in principle in vitro methylation-free Coomassie Brilliant Blue stain (2010)
  • Author(s): Yasumitsu H,Ozeki Y, Kawsar SMA, Toda T, Kanaly RA
  • Journal Title: Anal. Biochem Volume: 406 Pages: 86-88
  • DOI: DOI:10.1016/j.ab.2010.06.035
• [Journal Article] RAMA stain: A fast, sensitive and less protein modifying CBB R250 stain (2010)
  • Author(s): Yasumitsu H, Ozeki Y, Kawsar SMA, Fujii Y, Sakagami M, Matsuo Y, Toda T, Katsuno H
  • Journal Title: Electrophoresis 31 Volume: 31 Pages: 1913-1917
  • DOI: DOI:10.1002/elps.200900524
• [Journal Article] Production of Active MMP7 in E. coliand its application for metalloproteinase inhibitors screening (2010)
  • Author(s): Katsuno H, Shirakawa R, Miyazaki K, Ozeki Y, Yasumitsu H
  • Journal Title: Protein Pept. Lett. Volume: 17 Pages: 568-572
  • DOI: DOI:http://dx.doi.org/10.2174/092986610791112648
• [Journal Article] Inhibitory effects of RCG1, a β-galactoside-binding lectin from Rana catesbeiana(American bullfrog) oocytes against human and phytopathogens (2010)
  • Author(s): Kawsar SMA, Mamun SMA, Rahman MS, Yasumitsu H, Ozeki Y
  • Journal Title: Canadian J. Pure Appl. Sci Volume: 4 Pages: 1141-1149
• [Journal Article] Benz[a]anthracene biotransformation and production of ring fission products by Sphingobium sp. strain KK22
  • Author(s): Kunihiro M, Ozeki Y, Nogi Y, Hamamura N, Kanaly RA.
  • Journal Title: Appl Environ Microbiol
  • DOI: DOI:10.1128/AEM.01129-13
• [Presentation] Depletion of multidrug transporter protein on cancer cells caused by lectin-glycosphingolipid interaction enhanced the chemotheraputic effects (2012)
  • Author(s): Fujii Y, Sugawara, S., Nitta, K, Hosono M, Tatsuta T, Ogawa Y, Kawsar SMA, Matsumoto R, Koide Y, Hasan I, Hakomori S-I, Ozeki Y
  • Organizer: 第26回国際糖質学会
  • URL: http://ics2012madrid.com/
  • Place of Presentation: スペイン共和国マドリッド市
  • Year and Date: 20120722-27
• [Presentation] Hyper-diversification glycan-recognizing patterns and cell regulative activities of galactophilic lectins from marine invertebrtates (2011)
  • Author(s): Matsumoto R, Shibata FT, Kawsar SMA, Fujii Y, Yasumitsu H, Ozeki Y.
  • Organizer: 24thInternational Lectin Conference
  • Place of Presentation: 豪州ブリスベン市
  • Year and Date: 20110727-29
• [Presentation] Glycomics study on marine invertebrate lectins to find the cell regulative activity (2010)
  • Author(s): Matsumoto R, Kawsar SMA, Fujii Y, Hasan I, Ozeki Y
  • Organizer: 9th International Marine Biotechnology Conference(第9回国際マリンバイオテクノロジー学会)
  • Place of Presentation: 中国、青島市
  • Year and Date: 20101008-12
• [Book] Marine Proteins and Peptides: Biological Activities and Applications (2013)
  • Author(s): Se-Kwon Kim
  • Total Pages: 167-184ISBN:978-1-1183-7506-8
  • Publisher: Wiley-Blackwell (Thomson Oxford, UK)
• [Book] Close Up実験法Series222 CGP染色:無臭・安価で迅速な高感度SDSゲル染色法実験医学30巻
  • Author(s): 頁安光英太郎、戸田年総、大関泰裕
  • Total Pages: 111-117
• [Remarks]
  • URL: http://en.wikipedia.org/wiki/Galactose_binding_lectin_domain
• [Remarks]
  • URL: http://www.uniprot.org/uniprot/B3EWR1
• [Remarks]
  • URL: http://www.shimoda.tsukuba.ac.jp/~jambio/ronbun.html#ozeki24
• [Remarks]
  • URL: http://www.shimoda.tsukuba.ac.jp/~jambio/ronbun.html#ozeki201305
• [Remarks]
  • URL: http://jglobal.jst.go.jp/detail.php?JGLOBAL_ID=201202270059150450&q=cgp%E6%9F%93%E8%89%B2&t=0
• [Remarks]
  • URL: http://www.bioregen.co.jp/products/item/rbs02.html