Development of transgenic animals using a human artificial chromosome (HAC) vector

2012 Fiscal Year Final Research Report

• Project/Area Number: 22580320
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Yamaguchi University
• Principal Investigator: OTOI Takeshige 山口大学, 共同獣医学部, 教授 (30311814)
• Project Period (FY): 2010 – 2012
• Keywords: 遺伝子改変動物 / 人工染色体 / クローン / 蛍光タンパク質 / ネコ

Research Abstract

We established the two cell lines of cat fibroblasts with a human artificial chromosome (HAC) vector containing the green fluorescent protein (GFP). By inserting the transgenic cell into an enucleated cat egg, the cloned embryos were produced. The ability for cloned embryos to develop to blastocysts was higher in the short-time fusion group (30 μsec) than in the long-time fusion group (60 μsec). The embryos were transferred to 13 recipients. Only one recipient became pregnant and delivered a live kitten (74g).

Research Products

• [Journal Article] Histone deacetylase inhibitor improves the development and acetylation levels of cat cow interspecies cloned embryos (2013)
  • Author(s): Wittayarat, M., Sato, Y., Do, T.K.L., Morita, Y., Chatdarong, K., Techakumphu, M., Taniguchi, M. and Otoi, T.
  • Journal Title: Cellular Reprogramming
  • Peer Reviewed
• [Journal Article] Cell cycle analysis and interspecies nuclear transfer of cat cells treated with chemical inhibitors (2013)
  • Author(s): Wittayarat, M., Fujiwara, A., Chatdarong, K., Techakumphu, M., Sato, Y., Tanihara, F., Morita, Y., Taniguchi, M. and Otoi, T
  • Journal Title: Acta Veterinaria Hungarica
  • Peer Reviewed
• [Journal Article] The effect of relaxin supplementation of in vitro maturation medium on the development of cat oocytes obtained from ovaries stored at 4 °C (2013)
  • Author(s): Luu, V.V., Hanatate, K., Tanihara, F., Sato, Y., Do, T.K.L., Taniguchi, M., and Otoi, T.
  • Journal Title: Reproductive Biology
  • Peer Reviewed
• [Journal Article] Comparison of activation ability between feline and bovine oocytes (2013)
  • Author(s): Tanihara, F., Kaedei, Y., Namula, Z., Luu, V.V., Sato, Y., Wittayarat, M., Taniguchi, M. and Otoi, T
  • Journal Title: Acta Veterinaria Hungarica
  • Peer Reviewed
• [Journal Article] Cell cycle synchronization of skin fibroblast cells in four species of family Felidae (2013)
  • Author(s): Wittayarat, M., Thongphakdee, A., Saikhun, K., Chatdarong, K., Otoi, T.and Techakumphu, M
  • Journal Title: Reproduction in Domestic Animals Volume: 48 Pages: 305-310
  • Peer Reviewed
• [Journal Article] Development and subsequent cryotolerance of domestic cat embryos cultured in serum-free and serum-containing media (2011)
  • Author(s): Murakami, M., Dong, Y.J., Suzuki, T., Taniguchi, M., Kaedei, Y., Sato, Y., Tanihara, F. and Otoi, T
  • Journal Title: Cryobiology Volume: 63 Pages: 170-174
  • Peer Reviewed
• [Journal Article] Antiviral restriction factor transgenesis in the domestic cat. (2011)
  • Author(s): Wongsrikeao, P., Saenz, D., Rinkoski, T., Otoi, T. and Poeschla, E
  • Journal Title: Nature Methods Volume: 8 Pages: 853-859
  • Peer Reviewed
• [Presentation] TEffect of trichostatin A on in vitro embryo development of interspecies nuclear transfer embryos reconstructed from cat donor nuclei and bovine cytoplasm. 39th Annual Conference of the IETS (2013)
  • Author(s): Wittayarat, M., Namula, Z., Luu, V. V., Do, L. T. K., Sato, Y., Taniguchi, M. and Otoi
  • Organizer: 39th Annual Conference of the IETS
  • Place of Presentation: Hannover, Germany
  • Year and Date: 2013-01-20
• [Presentation] Effects of ovary storage time on the quality and meiotic competence of cat oocytes (2012)
  • Author(s): Luu, V. V., Namula, Z., Kaedei, Y., Tanihara, F. and Otoi, T
  • Organizer: 38th Annual Conference of the IETS
  • Place of Presentation: Phoenix, AZ, USA
  • Year and Date: 2012-01-08