2012 Fiscal Year Final Research Report
Activation mechanism of cyclic ADP-ribose-synthesizing enzyme CD38
| Project/Area Number |
22590202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Kanazawa University |
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Principal Investigator |
MINAKO Hashii 金沢大学, 子どものこころの発達研究センター, 協力研究員 (10272957)
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| Co-Investigator(Kenkyū-buntansha) |
HIGUCHI Yoshihiro 鈴鹿医療科学大学, 薬学部, 教授 (10019630)
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| Co-Investigator(Renkei-kenkyūsha) |
HIGASHIDA Haruhiro 金沢大学, 医学系, 教授 (30093066)
MATSUKAWA Shigeru 福井大学, 学内共同利用施設 等, 准教授 (00092809)
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| Project Period (FY) |
2010 – 2012
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| Keywords | CD38 / サイクリックADPリボース / カルシウムイオン / 結合蛋白 / プロテオミクス |
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| Research Abstract |
Human CD38 has enzymatic function that catalyzes multiple reactions to produce Ca2+-mobilizing second messenger, cyclic ADP-ribose. In this study, we try to detect CD38-interacting proteins those may influence CD38 activity using proteomic analysis. We find protein kinase (PK) as a binding partner of CD38, and show that enhanced Ca2+signaling including cytosolic Ca2+oscillations were suppressed by inhibition of PK. Therefore it is suggested that PK is involved in activation mechanism of CD38.
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[Journal Article] Oxytocininduced elevation of ADP-ribosyl cyclase activity, cyclic ADP-ribose or Ca(2+) concentrations is involved in autoregulation of oxytocin secretion in the hypothalamus and posterior pituitary in male mice2010
Author(s)
Lopatina,O.,Liu,H.X.,Amina,S., Hashii,M.,Higashida,H
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Journal Title
Neuropharmacology
Volume: 58
Pages: 50-55
DOI
Peer Reviewed
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[Journal Article] Intracellular calcium elevation induced by extracellular application of cyclic-ADP-ribose or oxytocin is temperature-sensitive in rodent NG108-15 neuronal cells with or without exogenous expression of human oxytocin receptors2010
Author(s)
Amina,S.,Hashii,M.,Ma,W.J.,Yokoyama,S.,Lopatina,O.,Liu,H.X.,Islam,M.S.,Higashida,H
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Journal Title
J. Neuroendocrinol
Volume: 22
Pages: 460-466
DOI
Peer Reviewed
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