Dynamical analysis of budding process in influenza virus infection by its visualization

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590420
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Nihon University
• Principal Investigator: KURODA Kazumichi 日本大学, 医学部, 准教授 (50215109)
• Co-Investigator(Kenkyū-buntansha): SHIBATA Toshikatu 日本大学, 医学部, 研究員 (30398854)
• Project Period (FY): 2010 – 2012
• Keywords: 細胞 / 出芽

Research Abstract

By using fluorescence-protein-tagged M1 protein (GFP-M1) and total internal reflection microscope, we tried to observe the process of influenza viral particle formation in live cells. When GFP-M1 expressing cells were infected with the virus, punctate structure of GFP-M1 was observed after 8 hours infection, and then the structures tended to aggregate. Another viral protein, HA showd the same tendency. The results indicated the punctate sturucture of GFP-M1 corresponded to theformation of viral particle.

Research Products

• [Journal Article] polyphenol-rich extract from Chaenomeles sinensis (Chinese quince) inhibits influenza A virus infection by preventing primary transcription in vitro. (2013)
  • Author(s): Sawai-Kuroda R, Kikuchi S, ShimizuYK, Sasaki Y, Kuroda K, Tanaka T, Yamamoto T, Sakurai K, Shimizu K.,A
  • Journal Title: J Ethnopharmacol Volume: 146(3) Pages: 866-872
  • Peer Reviewed
• [Journal Article] virus NS4B targets lipid droplets through hydrophobic residues in the amphipathic helices. (2013)
  • Author(s): Tanaka T, Kuroda K, Ikeda M, WakitaT, Kato N, Makishima M. Hepatitis C
  • Journal Title: J Lipid Res. Volume: 54(4) Pages: 881-892
  • Peer Reviewed
• [Journal Article] Bacterialneuraminidase rescues influenza virus replication from inhibition by a neuraminidase inhibitor. (2012)
  • Author(s): Nishikawa T, Shimizu K, Tanaka T, Kuroda K, Takayama T, Yamamoto T,Hanada N, Hamada Y.
  • Journal Title: PLoS One. Volume: 7(9) Pages: e45371
  • Peer Reviewed
• [Journal Article] The interaction between Lyn andFcεRIβ is indispensable for FcεRI-mediated human mast cell activation. (2012)
  • Author(s): Okayama Y, Kashiwakura JI, Matsuda A, Sasaki-Sakamoto T,Nunomura S, Yokoi N, Ebihara N,Kuroda K, Ohmori K, Saito H, Ra C.
  • Journal Title: Allergy. Volume: 67(10) Pages: 1241-1249
  • Peer Reviewed
• [Journal Article] H1N1/09 influenza Avirus infection of immortalized firsttrimester human trophoblast cell lines. (2012)
  • Author(s): Komine-Aizawa S, Suzaki A, Trinh QD, Izumi Y, Shibata T, Kuroda K, Hayakawa S.
  • Journal Title: Am J Reprod Immunol. Volume: 68(3) Pages: 226-232
  • Peer Reviewed
• [Journal Article] Peroxiredoxin family proteins are key initiators of post-ischemic inflammation in the brain. (2012)
  • Author(s): Shichita T, Hasegawa E, Kimura A, Morita R, Sakaguchi R, Takada I, Sekiya T, Ooboshi H, Kitazono T,Yanagawa T, Ishii T, Takahashi H,Mori S, Nishibori M, Kuroda K, AkiraS, Miyake K, Yoshimura A.
  • Journal Title: Nat Med. Volume: 18(6) Pages: 911-917
  • Peer Reviewed
• [Journal Article] SOCS1regulates type I/type II NKT cell balance by regulating IFNgamma signaling. (2011)
  • Author(s): Hashimoto M, Hiwatashi K, Ichiyama K, Morita R, Sekiya T, Kimura A, Sugiyama Y, Sibata T, Kuroda K,Takahashi R, Yoshimura A.
  • Journal Title: Int Immunol Volume: 23(3) Pages: 165-176
  • Peer Reviewed
• [Journal Article] 宿主細胞内への侵入:エンドサイトーシス (2010)
  • Author(s): 芝田敏克、黒田和道
  • Journal Title: 現代化学 Volume: 11月号 Pages: 24-27
• [Presentation] インフルエンザウイルスM1タンパク質と核内構造物ND10との相互作用の解析 (2012)
  • Author(s): 芝田敏克、豊澤恵子、早川智、山本樹生、清水一史、黒田和道
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-14
• [Presentation] UBE2L6DOWN-REGULATES INFLUENZAVIRUS REPLICATION. (2011)
  • Author(s): Shimotai Y, Shibata T, Sasaki Y, Saito M, Kuroda K, Tanaka T, Hongo S, Hayakawa S, Shimizu K.
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-12
• [Presentation] BACTERIALNEURAMINIDASE REDUCES THE ANTIVIRALEFFECTS OF INFLUENZA VIRUS NEURAMINIDASE INHIBITOR. (2011)
  • Author(s): Nishikawa T, Toyosawa K, Kuroda K, Yamamoto T, Hanada N, Hamada Y,Shimizu K.
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-11
• [Presentation] DIFFERENT IMAGES OF INFLUENZA VIRUSM1 PROTEIN AT BUDDING SITESOBTAINED BY IMMUNOSTAINING AND TETRACYSTEINE-TAGSTAINING. (2011)
  • Author(s): Shibata T, Hayakawa S, Shimizu K, Yamamoto T, Kuroda K.
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-11
• [Presentation] INFLUENZA VIRUS M1 PROTEIN ACCUMULATES IN THE SUBNUCLEAR STRUCTURE, ND10, WITHOUT ANY HELP FROM OTHER VIRAL PROTEINS. (2011)
  • Author(s): Shibata T, Hayakawa S, Shimizu K, Yamamoto T, Kuroda K.
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-11
• [Presentation] インフルエンザウイルスM1タンパク質の核内構造物ND10への集積 (2010)
  • Author(s): 芝田敏克、清水一史、早川智、山本樹生、黒田和道
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-07