2012 Fiscal Year Final Research Report

Influence of prior pandemic A(H1N1)2009 virus infection on invasion of MDCK cells by community-acquired MRSA

Research Project

Project/Area Number	22590514
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Laboratory medicine
Research Institution	Kitasato University
Principal Investigator	TAKAYAMA Yoko 北里大学, 医学部, 講師 (80286278)
Co-Investigator(Kenkyū-buntansha)	YANO Hisakazu 東北大学, 大学院・医学系研究科, 講師 (20374944) ARAI Kazuaki 東北大学, 大学院・医学系研究科, 研究補佐 (30547386) HIRAKATA Yoichi 東北大学, 大学院・医学系研究科, 教授 (50238341)
Project Period (FY)	2010 – 2012
Keywords	A(H1N1)pdm09 / community-acquired MRSA / invasion
Research Abstract	In this study, we investigated whether prior infection with pandemic A(H1N1)2009 virus (A(H1N1)pdm09) led to increased invasion of airway epithelial cells by CA-MRSA. We performed the gentamicin killing assay using Madin-Darby canine kidney (MDCK) cells and various bacteria to investigate whether prior A(H1N1)pdm09 infection led to increased invasion of these epithelial cells by CA-MRSA. We found that the invasion rate of two MRSA strains (ATCC BAA-1680 (USA 300) and ATCC BAA-1699 (USA 100)) into intact MDCK cell monolayers was 0.29±0.15% and 0.007±0.002%, respectively (p=3). In addition, the relative invasion rate of both ATCC BAA-1680 and ATCC BAA-1699 was significantly increased by prior A(H1N1)pdm09 infection of MDCK monolayers from 1±0.28 to 1.38±0.02 and from 1±0.24 to 1.73±0.29, respectively (p