Evaluation of Clathrin Heavy Chain (new tumor marker) for diagnosis in various cancer

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590518
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Chiba University
• Principal Investigator: SEIMIYA Masanori 千葉大学, 医学部附属病院, 主任臨床検査技師 (20554265)
• Co-Investigator(Kenkyū-buntansha): NOMURA Fumio 千葉大学, 大学院・医学研究院, 教授 (80164739) ; MATSUSHITA Kazuyuki 千葉大学, 大学院・医学研究院, 准教授 (90344994)
• Co-Investigator(Renkei-kenkyūsha): TOMONAGA Takeshi 独立行政法人医薬基盤研究所, プロジェクト リーダー (80227644) ; KODERA Yoshio 北里大学, 理学部, 准教授 (60265733) ; OH-ISHI Masamichi 北里大学, 理学部, 講師 (40233027)
• Project Period (FY): 2010 – 2012
• Keywords: 悪性腫瘍 / 病理組織診断 / クラスリン重鎖 / 免疫組織化学染色

Research Abstract

We investigated the usefulness of immunohistochemical staining of CHC for the histological diagnosis of various cancer tissues. Since an apparent difference was noted in CHC expression between cancerous and non-cancerous regions of esophageal cancer, we investigated the usefulness for the histopathological diagnosis of esophageal cancer. As a result, we found that CHC was significantly more strongly expressed in the cytoplasm of malignant legions, than in that of normal tissue or benign lesions. Moreover, we thought the serum autoantibodies of CHC will be detectable in HCC patients. Serum levels of anti-CHC antibodies were significantly elevated in HCC patients compared to those in liver cirrhosis patients.

Research Products

• [Journal Article] Child-pugh score is altered by the albumin measurement method (2013)
  • Author(s): Seimiya M, Ohno S, Yamamoto H, Fujiwara K, Yoshida T, Sawabe Y, Sogawa K, Matsushita K, Yokosuka O, Nomura F
  • Journal Title: Hepatology Volume: 57(5) Pages: 2093-4
  • DOI: doi:10.1002/hep.25972.Epub 2012 Sep 24.
• [Journal Article] Combined proteomic analysis of liver tissue and serum in chronically alcohol-fed rats (2013)
  • Author(s): Yamada M, Satoh M, Seimiya M, Sogawa K, Itoga S, Tomonaga T, Nomura F
  • Journal Title: Alcohol Clin Exp Res Volume: 37 Pages: E79-87
  • DOI: doi:10.1111/j.1530-0277.2012.01883.x.
• [Journal Article] Serum cystatin C as a marker for early detection of chronic kidney disease and grade 2 nephropathy in Japanese patients with type 2 diabetes (2012)
  • Author(s): Suzuki Y, Matsushita K, Seimiya M,Yoshida T, Sawabe Y, Ogawa M, Nomura F
  • Journal Title: Clin Chem Lab Med Volume: 50(10) Pages: 1833-9
  • DOI: doi:10.1515/cclm-2011-0777.
• [Journal Article] Serum anti-Ku86 is a potential biomarker for early detection of hepatitis C virus-related hepatocellular carcinoma (2012)
  • Author(s): Nomura F, Sogawa K, Noda K, Seimiya M, Matsushita K, Miura T, Tomonaga T, Yoshitomi H, Imazeki F, Takizawa H, Mogushi K, Miyazaki M, Yokosuka O
  • Journal Title: Biochem Biophys Res Commun Volume: 421(4) Pages: 837-43
  • DOI: doi:10.1016/j.bbrc.2012.04.099.
• [Journal Article] Decreases in the serum VLDL-TG/non-VLDL-TG ratio from early stages of chronic hepatitis C: alterations in TG-rich lipoprotein levels (2011)
  • Author(s): Nishimura M, Yamamoto H, Yoshida T, Seimiya M, Sawabe Y, Matsushita K, Umemura H, Sogawa K, Takizawa H, Yokosuka O, Nomura F
  • Journal Title: PLoS One Volume: 6(2) Pages: e17309
  • DOI: doi:10.1371/journal.pone.0017309
• [Journal Article] Reducing the incidence of pseudohyper- kalemia by avoiding making a fist during phlebotomy: a quality improvement report (2010)
  • Author(s): Seimiya M, Yoshida T, Sawabe Y, Sogawa K, Umemura H, Matsushita K, Nomura F
  • Journal Title: Am J Kidney Dis Volume: 56(4) Pages: 686-92
  • DOI: doi:10.1053/j.ajkd.2010.06.014
• [Journal Article] Application of Fourier-transform infrared (FT-IR) spectroscopy for simple and easy determination of chylomicron- triglyceride and very low density lipo- protein-triglyceride (2010)
  • Author(s): Sato K, Seimiya M, Kodera Y, Kitamura A, Nomura F
  • Journal Title: Clin Chim Acta Volume: 411(3-4) Pages: 285-90
  • DOI: doi:10.1016/j.cca.2009.11.026.
• [Journal Article] Serum fibrinogen alpha C-chain 5.9 kDa fragment (FIC 5.9) as a biomarker for early detection of hepatic fibrosis related to hepatitis C virus
  • Author(s): Sogawa K, Noda K, Umemura H, Seimiya M, Kuga T, Tomonaga T, Nishimura M, Kanai F, Imazeki F, Takizawa H, Yoneda M, Nakajima A, Tsutsumi M, Yokosuka O, Nomura F
  • Journal Title: Proteomics Clin Appl Volume: (in press)
  • DOI: doi:10.1002/prca.201200094.
• [Journal Article] Preanalytical evaluation of serum 25-hydroxyvitamin D3 and25-hydroxyvitamin D2 measurements using LC-MS/MS
  • Author(s): Mochizuki A, Kodera Y, Saito T, Satoh M, Sogawa K, Nishimura M, Seimiya M, Kubota M, Nomura F
  • Journal Title: Clin Chim Acta Volume: (in press)
  • DOI: doi:pii: S0009-8981(12)00508-6. 10.1016/j.cca.2012.10.041.
• [Presentation] クラスリン重鎖の組織診断マーカーとしての有用性の検討 (2013)
  • Author(s): 清宮正徳、時田和也、朝長毅、松下一之、中谷行雄、野村文夫
  • Organizer: 第23回生物試料分析科学会年次学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20130000
• [Presentation] Roles of Clathrin Heavy Chain Immunohistochemistry for Esophageal Squamous Intraepithelial Neoplasia - Observations Originated from Proteomic Studies (2012)
  • Author(s): Seimiya M, Tokita K, Matsushita K, Tomonaga T, Onodera K, Ohki S, Tanizawa T,Shimada H, Uesato M, Matsubara H, Nakatani Y, Nomura F
  • Organizer: HUPO (Human Proteome Organisa- tion) 11th Annual World Congress
  • Place of Presentation: Boston
  • Year and Date: 20120000