Effect of prenatal environment on stress response in offspring

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591207
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Showa University
• Principal Investigator: OGAWA Tetsuo 昭和大学, 医学部, 講師 (60384210)
• Co-Investigator(Kenkyū-buntansha): SHIODA Seiji 昭和大学, 医学部, 教授 (80102375) ; KAGEYAMA Hruaki 桐生大学, 医療保健学部, 准教授 (00433839)
• Project Period (FY): 2010 – 2012
• Keywords: DOHaD / 子宮内環境 / 低栄養 / 生活習慣病 / 胎児期低栄養 / DNAマイクロアレイ1

Research Abstract

Prenatal undernutrition has been associated with increased risks of adult diseases. We treated pregnant mice with 50% food restriction, and performed global gene expression and promoter DNA methylation profiling on the fetal livers. We successfully found genes such as trib1 which has been associated with heart diseases, and many genes related to the immune system, and glucocorticoid signaling. These searched genes may be useful to predict nutritional environment during fetal period, and risks of adult diseases due to fetal environments.

Research Products

• [Journal Article] Transcriptomics and proteomics analyses of the PACAP38 influenced ischemic brain in permanent middle cerebral artery occlusion model mice (2012)
  • Author(s): Hori M, Nakamachi T, Rakwal R, Shibato J, Ogawa T, Aiuchi T, Tsuruyama T, Tamaki K, Shioda S
  • Journal Title: J Neuroinflammation Volume: 9 Pages: 256
  • DOI: doi:10.1186/1742-2094-9-256.
• [Journal Article] Shared features of S100B immunohistochemistry and cytochrome oxidase histochemistry in the ventroposterior thalamus and lateral habenula in neonatal rats (2012)
  • Author(s): Muneoka K, Funahashi H, Ogawa T, Whitaker-Azmitia PM, Shioda S.
  • Journal Title: Int. J. Dev. Neurosci Volume: 30 Pages: 499-505
  • DOI: doi:10.1016/j.npep.2012.09.007
• [Journal Article] Abnormal brain function of the rat neonate in a prenatal 5-bromo-2'-deoxyuridine (BrdU)-induced developmental disorder model (2012)
  • Author(s): Ogawa T, Kuwagata M, Muneoka K, Wakai C, Senuma M, Kubo H, Shioda S.
  • Journal Title: Int. J. Dev. Neurosci Volume: 30 Pages: 207-15
  • DOI: doi:10.1016/j.ijdevneu.2012.05.002
• [Journal Article] Hyperactivity induced by prenatal BrdU exposure across several experimental conditions (2011)
  • Author(s): Kuwagata M, Ogawa T, Shioda S
  • Journal Title: Congenit. Anom.Kyoto Volume: 51 Pages: 177-182
  • DOI: doi:10.1111/j.1741-4520.2011.00331.x.
• [Journal Article] Effects of the genotoxic agent 5-bromo-2'-deoxyuridine with or without pre-pubertal gonadectomy on brain monoamines and their metabolites in female rats (2011)
  • Author(s): Kuwagata M, Muneoka K, Ogawa T, Shioda S.
  • Journal Title: Brain Res Bull Volume: 85 Pages: 207-211
  • DOI: doi:10.1016/j.brainresbull.2011.03.005
• [Journal Article] Seeking Gene Candidates Responsible for Developmental Origins of Health and Disease (DOHaD) (2011)
  • Author(s): Ogawa T, Rakwal R, Shibato J, Sawa C, Saito T, Murayama A, Kuwagata M, KageyamaH, Yagi M, Satoh K, Shioda S
  • Journal Title: Congenit. Anom.Kyoto Volume: 51 Pages: 110-125
  • DOI: doi:10.1111/j.1741-4520.2011.00315.x.
• [Journal Article] Distribution of the longevity gene product, SIRT1, in developing mouse organs. (2011)
  • Author(s): Ogawa T, Wakai C, Saito T, Murayama A, Mimura Y, Youfu S, Nakamachi T, Kuwagata M, Satoh K, Shioda S
  • Journal Title: Congenit. Anom.Kyoto Volume: 51 Pages: 70-79
  • DOI: doi:10.1111/j.1741-4520.2010.00304.x
• [Journal Article] Sex-specific effects of early neonatal progesterone treatment on dopamine and serotonin metabolism in rat striatum and frontal cortex (2010)
  • Author(s): Muneoka K, Kuwagata M, Ogawa T, ShiodaS.
  • Journal Title: Life Sci. Volume: 87 Pages: 738-742
  • DOI: doi:10.1016/j.lfs.2010.10.016.
• [Presentation] バルプロ酸の胎生期曝露により実験動物に誘発される発達神経毒性 (2012)
  • Author(s): 小川哲郎
  • Organizer: 第52回日本先天異常学会
  • Place of Presentation: 東京
  • Year and Date: 2012-07-07
• [Presentation] Searching gene candidates responsible for mental disorders in the fetal mouse undernutrition model (2011)
  • Author(s): Ogawa T
  • Organizer: 27th International Neurotoxicology Conference
  • Place of Presentation: Research Triangle Park, NC
  • Year and Date: 2011-12-01
• [Presentation] Effect of nucleoprotein (NP) diet on expression of gene candidates for developmental origins of health and disease (DOHaD). (2011)
  • Author(s): Ogawa T
  • Organizer: Developmental origins of health and disease 7th world congress
  • Place of Presentation: Portland
  • Year and Date: 2011-09-20
• [Presentation] Global gene expression profiling analysis of food deprived maternal and fetal mice livers (2011)
  • Author(s): Ogawa T
  • Organizer: 50th Society of Toxicology
  • Place of Presentation: Washington DC
  • Year and Date: 2011-03-08
• [Presentation] Unraveling the effects of development on the olfactory system in a BrdU-induced developmental disorder model rat (2011)
  • Author(s): Kuwagata M, Ogawa T
  • Organizer: 50th Society of Toxicology
  • Place of Presentation: Washington DC
  • Year and Date: 2011-03-08
• [Book] Rakwal R Brain Proteomics: Sample Preparation Techniques for the Analysis of Rat Brain Samples Using Mass Spectrometry, In Sample Preparation in Biological Mass Spectrometry (2011)
  • Author(s): Masuo Y, Hirano M, Shibato J, Nam H W, Fournier I, Meriaux C, Wisztorski M, Salzet M, Soya H, Agrawal GK, Ogawa T, Shioda S
  • Total Pages: 171-195
  • Publisher: Springer