2012 Fiscal Year Final Research Report
Development of radiolabeled antibody for channel transporter imaging
| Project/Area Number |
22591372
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Ibaraki Prefectural University of Health Science |
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Principal Investigator |
SHIMAMOTO Naoto 茨城県立医療大学, 保健医療学部, 講師 (80295435)
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| Co-Investigator(Renkei-kenkyūsha) |
UEHARA Tomoya 千葉大学, 薬学研究院, 准教授 (10323403)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 放射性医薬品 / 造影剤 |
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| Research Abstract |
Several hundred human channel/transporter genes reportedly haveconnections with nervous diseases or other diseases, including cancer . In recent years, many kinds ofpolyclonal and/or monoclonal antibodies to channel/transporters have been developed. Using theseantibodies, development of radiolabeled antibodies suitable for diagnosis and treatment has beenanticipated. We planned to develop a neutral-amino-acid transporter imaging agent as a radiolabeledantibody targeting L-type amino acid transporter 1 (LA T1), which is expressed in many kinds of tumor .For the evaluation of this radiolabeled antibody , we used nude mice transplanted with cells from theDLD-1 human colorectal cancer cell line. Expressions of proteins, such as the amino acid transporterand its chaperone-like proteins (4F2hc, LA T1, LA T2, LA T3, LA T4, A T A1, A T A2, ASCT1, ASCT2,MCT8, T A T1, and B0A T1), were examined using an established method with real-time polymerase chainreaction. The new monoclonal antibody for immunohistochemical analysis was evaluated using theDLD-1 transplanted nude mice after125I-rabeling. This antibody was able to detect the LA T1 protein ofthe internality level with tumor tissue sections in immunohistochemical analysis, but was not suitable forin vivo tumor detection, most likely because the antibody specifically associates with the N-endnear-field region of the human LA T1 protein. We are planning in vivo imaging using antibody againsthuman CD98 (4F2hc).
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Research Products
(18 results)
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[Journal Article] Appropriate parameters of theordered-subset expectationmaximization algorithm on measurementof myocardial blood flow and oxygen consumption with11C-acetate PET2012
Author(s)
M Kobayashi, T Mori, Y Kiyono, TTsujikawa, R Maruyama, Y Higaki, NShikano, R Nishii, K Kawai, T Kudo, Hokazawa
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Journal Title
NuclMed Commun
Volume: 33
Pages: 130-138
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[Journal Article] Putative transport mechanism andintracellular fate of anti-18F-FACBC(trans-1-amino-3-18F-fluorocyclobutanecarboxylic acid) in human prostatecancer2011
Author(s)
H Okudaira, N Shikano, R Nishii, TMiyagi, M Yoshimoto, M Kobayashi, K Ohe,T Nakanishi, I Tamai, M Namiki, K Kawai
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Journal Title
J Nucl Med
Volume: 5
Pages: 2822-2829
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[Journal Article] Comparison of the transcellulartransport of FDG and D-glucose by theKidney epithelial cell line LLC-PK12010
Author(s)
M Kobayashi, N Shikano, R Nishii, YKiyono, H Araki, K Nishi, M Oh, HOkudaira, M Ogura, M Yoshimoto, HOkazawa, Y Fujibayashi, K Kawai
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Journal Title
Nucl Med Commun
Volume: 31
Pages: 141-146
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[Journal Article] Uptake of3-[125I]iodo-α-methyl-l-tyrosine intocolon cancer DLD-1 cells:characterization and inhibitoryeffect of natural amino acids and aminoacid-like drugs2010
Author(s)
N Shikano, M Ogura, H Okudaira, SNakajima, T Kotani, M Kobayashi, SNakazawa, T Baba, N Yamaguchi, N Kubota, Y Iwamura, K Kawai
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Journal Title
Nucl Med Biol
Volume: 37
Pages: 197-204
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[Journal Article] Radioiodinated4-iodo-L-meta-tyrosine, a system Lselective artificial amino acid:Molecular design and transportcharacterization in Chinese hamsterovary cells (CHO-K1 cells)
Author(s)
N Shikano, T Kotani, S Nakajima, MOgura, S Nakazawa, J Sagara, MKobayashi, T Baba, N Yamaguchi, NKubota, K Kawai
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Journal Title
Nucl MedBiol
Volume: 37
Pages: 903-910
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[Journal Article] Pharmacokinetic Alteration of99mTc-MAG3 using Serum Protein BindingDisplacement Method
Author(s)
K Nishi, M Kobayashi, R Nishii, NShikano, N Takamura, N Kuga, K Yamasaki,S Nagamachi, S Tamura, M Otagiri, Kkawai
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Journal Title
Nucl Med Biol
Volume: 40
Pages: 366-370
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[Presentation] Intracellularboron accumulation in CHO-K1 cellsusing amino acid transport control2012
Author(s)
E Sato, T Yamamoto, N Shikano, M Ogura,K Nakai, F Yoshida, Y Uemae, T Takada,T Isobe, A Matsumura
Organizer
15th International Congress on NeutronCapture Therapy, 10 to 14 September2012 at the Tsukuba InternationalCongress Center
Year and Date
20120000
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[Presentation] Transport mechanism oftrans-1-amino-3-18F-fluorocyclobutanecarboxylic acid (18F-FACBC) in humanprostate cancer2012
Author(s)
H Okudaira, N Shikano, R Nishii, TMiyagi, M Yoshimoto, M Kobayashi, K Ohe,T Nakanishi, M Namiki, I Tamai, K Kawai
Organizer
International Symposium on Past,Present and Future of MolecularPharmacokinatics
Place of Presentation
東京
Year and Date
2012-01-17
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