Pathomehcanism of lumbar ligamentum flavum hypertrophy

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591636
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Teikyo University
• Principal Investigator: SAIRYO Koichi 帝京大学, 医学部, 准教授 (10304528)
• Co-Investigator(Kenkyū-buntansha): DEZAWA Akira 帝京大学, 医学部, 教授 (90237024)
• Project Period (FY): 2010 – 2012
• Keywords: 黄色靭帯肥厚 / 腰部脊柱管狭窄症 / 線維増殖性疾患

Research Abstract

The pathomechanism of hypertrophy of ligamentum flavum (LF) is still unknown. We hypothesized that the mechanism would be similar to that in the hypertrophic scar formation during the would repair of skin. The pathology is considered to be fibro-proliferative disorder (FPD). To confirm the hypothesis, we have investigated the similarity of the hypertrophy of LF and hypertrophic scar. Hypertrophied LF samples were taken during the lumbardecompression surgery. Masson Trichlome (MT) and Elastica van Gieson (EVG) stain were uded to understand the scar and fibrosis. The similarity of FPD was investigated using the immunohistochemistry of type 1 and 3 collagen and alfa-smooth muscle antigen (αSMA). In the hypertrophied LF, elastic fiber was stained sparse, especially along the dorsal aspect in LF. Instead, scar and fibroscic tissue was confirmed in the LF. At the scarring site, type 1 and 3 collagen was stained. The αSMA was also confirmed to be stained. Thus, hypertrophy of LF would be a type of the fibro-proliferative disorder. The other major matrix of the normal LF is type 1 collagen. We evaluated the bad crosslink in collagen named Pentocidine, which reduces the ligament elasticity . We confirmed the existence of the pentocidine in LF, especially at the cite where type 1 collagen showed abundant production.

Research Products

• [Journal Article] Long-term outcomes of lumbar posterior apophyseal end-plate lesions in children and adolescents (2012)
  • Author(s): K, Sairyo K, Katoh S, Takao S, Kosaka H, Yasui N
  • Journal Title: J Bone Joint Surg-A Volume: 94 Pages: e74
  • Peer Reviewed
• [Journal Article] Jack-knife stretching promotes flexibility of tight hamstrings after 4 weeks: a pilot study (2012)
  • Author(s): Sairyo K, Kawamura T, Mase Y, Hada Y, Sakai T, Hasebe K, Dezawa A
  • Journal Title: European J Orthop Surg Traumatol
  • Peer Reviewed
• [Journal Article] Conservative treatment for pediatric lumbar spondylolysis to achieve bone healing using a hard brace: what type and how long? (2012)
  • Author(s): Sairyo K, Sakai T, Yasui N, Dezawa A
  • Journal Title: J Neurosurg Spine Volume: 16 Pages: 610-4
  • Peer Reviewed
• [Journal Article] Painful lumbar spondylolysis among pediatric sports players: a pilot MRI study (2011)
  • Author(s): Sairyo K, Sakai T, Mase Y, Kon T, Shibuya I, Kanamori Y, Kosugi T, Dezawa A
  • Journal Title: Arch Orthop Trauma Surg Volume: 131 Pages: 1485-9
  • Peer Reviewed
• [Journal Article] Biomechanical rationale of sacral rounding deformity in pediatric spondylolisthesis: A clinical and biomechanical study (2011)
  • Author(s): Terai T, Sairyo K, Goel VK, Ebraheim N, Biyani A, Ahmad F, Kiapour A, HigashinoK, Sakai T, Yasui N
  • Journal Title: Arch Orthop Trauma Surg Volume: 131 Pages: 1187-94
  • Peer Reviewed
• [Journal Article] Operated and adjacent segment motions for fusion versus cervical arthroplasty: a pilot study (2011)
  • Author(s): Terai T, Faizan A, Sairyo K, Goel VK
  • Journal Title: Clin Orthop Relat Res Volume: 469 Pages: 682-7
  • Peer Reviewed
• [Presentation] Proteomic Analysis of Ligamentum Flavum from Lumbar Spinal Canal Stenosis (2013)
  • Author(s): Goda Y , Sato N, Taniguchi T , Takata Y , Kosaka H, Sakai T , Higashino K, SairyoK, Katoh S, Taniguchi H, Yasui N
  • Organizer: Annual Meeting of Orthopedic Research Socitety, San Antonio
  • Place of Presentation: TX, USA
  • Year and Date: 20130126-29
• [Presentation] Quantitative Proteomic Analysis of Human Tendon and Ligament (2012)
  • Author(s): Sato, N, Taniguchi T, Goda Y, Kosaka H, Higashino K, Sakai T , Sairyo K, Katoh S, Taniguchi H, Yasui N
  • Organizer: Annual Meeting of Orthopedic Research Socitety
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 20120204-07
• [Presentation] Establishment of a Method for Proteomic Analysis of Human Achilles Tendon (2011)
  • Author(s): Sato, N, Taniguchi T, Goda Y, Kosaka H, Higashino K, Sakai T , Sairyo K, Katoh S, Taniguchi H, Yasui N
  • Organizer: Annual Meeting of Orthopedic Research Socitety
  • Place of Presentation: Long Beach, CA, USA
  • Year and Date: 20110113-16
• [Presentation] The pathomechanism of the ligamentum flavum hypertrophy is similar to that of the hypertrophic scar formation during wound healing (2011)
  • Author(s): Sairyo K, Kanamori Y, Ogawa R, Goel V ,Biyani A, Ebraheim N, Dezawa A
  • Organizer: Annual Meeting of Orthopedic Research Socitety
  • Place of Presentation: Long Beach, CA, USA
  • Year and Date: 20110113-16
• [Presentation] Development of a Method for Proteomic Analysis of Human Yellow Ligament (2011)
  • Author(s): Sato, N, Taniguchi T, Goda Y, Kosaka H, Higashino K, Sakai T , Sairyo K, Katoh S, Taniguchi H, Yasui N
  • Organizer: Annual Meeting of Orthopedic Research Socitety
  • Place of Presentation: Long Beach, CA, USA
  • Year and Date: 20110113-16