2012 Fiscal Year Final Research Report
Induction of fluoroquinolone-resistant uropathogenic Escherichia coli using animal models
| Project/Area Number |
22591803
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KURAZONO Hisao 帯広畜産大学, 畜産学部, 教授 (90186487)
NOJIMA Michio 兵庫医科大学, 医学部, 教授 (90237842)
HIGUCHI Yoshihide 兵庫医科大学, 医学部, 講師 (20411994)
KANEMATU Akihiro 兵庫医科大学, 医学部, 准教授 (90437202)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 尿路病原性大腸菌、 / キノロン耐性 / QRDR遺伝子変異、 / 薬剤排出ポンプ |
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| Research Abstract |
Five fluoroquinolone-resistant (4- 32μ g/ml) Escherichia coli strains were developed by culture of 89 fluoroquinolone-susceptible isolates on agars including low to high concentration of levofloxacin. The original strains of these 5 resistant strains had one or two point-mutations in quinolone resistance-determining regions (QRDR). Further,the 5 resitant strains showed high RNA expression of marA, an efflux pump for antibiotics, determined by real time PCR. These results showed that a few ponint-mutations in QRDR and high expression of marA would be required as the first step for the acquisition of fluoroquinolone-resistance, indicating that inhibition of the efflux pump for antibiotics could be a good candidate for the strategy to prevent the induction of fluoroquinolone-resistance in treatment of urinary tract infections caused by uropathogenic E. coli.
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