Analyssis of the fate determination mechanism to corneal epithelial cells using fluorescent reporter

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591966
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Keio University
• Principal Investigator: YOSHIDA Satoru 慶應義塾大学, 医学部, 特任講師 (50398781)
• Co-Investigator(Renkei-kenkyūsha): SHIMMURA Shigeto 慶應義塾大学, 医学部, 准教授 (00235780)
• Project Period (FY): 2010 – 2012
• Keywords: 眼発生 / 再生医学 / 角膜上皮細胞 / ケラチン12

Research Abstract

We constructed a K12-DsRed BAC reporter, which express DsRed under the control of Krt12 promoter. The K12-DsRed reporter was introduced into a mouse corneal epithelial cell line, TKE2, and a mouse iPS cell line. An activity that converts bulge stem cells

Research Products

• [Journal Article] Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells (2011)
  • Author(s): Yoshida S, Yasuda M, Miyashita H, Ogawa Y, Yoshida T, Matsuzaki Y, Tsubota K, Okano H, Shimmura S
  • Journal Title: PLoS One Volume: 6(12) Pages: e28856
  • DOI: doi:10.1371/journal.pone.0028856
  • Peer Reviewed
• [Presentation] Differentiation Of Induced Pluripotent Stem Cell-Derived Neural Crest Cells Into Corneal Keratocytes In Vivo International Society for Stem Cell (2012)
  • Author(s): Yoshida S, Miyashita H, Ohta S, Yasuda M, Inagaki E, Tsubota K, Okano H, Shimmura S.
  • Organizer: 10th Annual Meeting, Yokohama
  • Place of Presentation: Japan
  • Year and Date: 20120613-16
• [Presentation] Generation of stratified squamous epithelial cells from induced pluripotent stem cells (2011)
  • Author(s): Yoshida S, Yasuda M, Miyashita H, Yoshida T, Okano H, Tsubota K, Shimmura S.
  • Organizer: The Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
  • Place of Presentation: Fort Lauderdale, FL, USA
  • Year and Date: 20110501-05
• [Presentation] Differentiation of induced pluripotent stem cells into corneal epithelial cells (2010)
  • Author(s): Yoshida S, Yasuda M, Miyashita H, Yoshida T, Tsubota K, Okano H, Shimmura S
  • Organizer: The 16th International Conference of the International Society of Differentiation
  • Place of Presentation: Nara, Japan
  • Year and Date: 20101115-18
• [Presentation] Differentiation of induced pluripotent stem cells into corneal epithelial cells. (2010)
  • Author(s): Yoshida S, Shimmura S, Okano H, Tsubota K.
  • Organizer: The Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
  • Place of Presentation: Fort Lauderdale, FL, USA
  • Year and Date: 20100502-06
• [Book] iPS 細胞からの角膜上皮誘導. 臨床眼科増刊 オキュラーサーフェス診療アップデート (2012)
  • Author(s): 羽藤晋, 吉田悟, 榛村重人
  • Total Pages: 323-326
  • Publisher: 医学書院