2011 Fiscal Year Final Research Report
Development of genetic manipulation technology for excitatory and inhibitory control of the target neuron activity
Project/Area Number |
22650065
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Neuroscience in general
|
Research Institution | Fukushima Medical University |
Principal Investigator |
|
Project Period (FY) |
2010 – 2011
|
Keywords | 脳機能 / 神経活動 / 細胞標的法 / 抗体 / リポソーム / プロテオリポソーム |
Research Abstract |
In the present study, we aimed to develop a novel technology for excitatory and inhibitory control of the activity of target neuronal types. A single chain variable region of anti-Tac monoclonal antibody against human interleukin-2 receptor a-subunit was fused to Fc fragment[anti-Tac(Fv)-Fc] was bound to liposome to produce immunoliposome. Anti-Tac(Fv) was connected to the transmembrane domain of vesicular somatitis virus glycoprotein or VSV-G[anti-Tac(Fv)-VSV-G], and anti-Tac(Fv)-VSV-G was expressed in insect cells by the baculoviral vector system. These cells were fused to loposome to generate proteoliposome containing anti-Tac(Fv)-VSV-G. We succeeded in the reduction of nonspecific binding of immunoliposome and proteoliposome by adding unlabelled control liposome into the solution containing the rhodamine-labelled liposome. This strategy will be useful to control the activity of neurons by introducing some drugs that inhibit ion channels into immunoliposome or proteoliposome.
|
-
[Journal Article] Selective neural pathway targeting reveals key roles of thalamostriatal projection in the control of visual discrimination2011
Author(s)
Kato, S., Kuramochi, M., Kobayashi, K., Fukabori, R., Okada, K., Uchigashima M., Watanabe, M., Tsutsui, Y., and Kobayashi, K.
-
Journal Title
J. Neurosci
Volume: 31(47)
Pages: 17169-171791
Peer Reviewed
-
[Journal Article] Sensory input regulates spatial and subtype-specific patterns of neuronal turnover in the adult olfactory bulb2011
Author(s)
Sawada, M., Kaneko, N., Wake, H., Inada, H., Kato, Y., Yanagawa, Y., Kobayashi, K., Nemoto, T., Nabekura, J. and Sawamoto, K.
-
Journal Title
J. Neurosci
Volume: 31(32)
Pages: 11587-11596
Peer Reviewed
-
[Journal Article] Postnatal development of tyrosine hydroxylasem RNA-expressing neurons in mouse neostriatum2011
Author(s)
Masuda, M., Miura, M., Inoue, R., Imanishi, M., Saino-Saito, S., Takada, M., Kobayashi, K., and Aosaki, T.
-
Journal Title
Eur. J. Neurosci
Volume: 34(9)
Pages: 1355-13671
Peer Reviewed
-
[Journal Article] A lentiviral strategy for highly efficient retrograde gene transfer by pseudotyping with fusion envelope glycoprotein2011
Author(s)
Kato, S., Kobayashi, K., Inoue, K., Kuramochi, M., Okada, T., Yaginuma, H., Morimoto, K., Shimada, T., Takada, M., and Kobayashi, K.
-
Journal Title
Hum. Gene Ther
Volume: 22(2)
Pages: 197-206
Peer Reviewed
-
[Journal Article] Rho/Rho-kinase signaling controls axon patterning of a specified subpopulation of cranial motor neurons2011
Author(s)
Kobayashi, K., Masuda, T., Takahashi, M., Miyazaki, J., Nakagawa, M., Uchigashima, M., Watanabe, M., Yaginuma, H., Osumi, N., Kaibuchi, K., and Kobayashi, K.
-
Journal Title
Eur. J. Neurosci
Volume: 33(4)
Pages: 612-621
Peer Reviewed
-
-
-
-