2011 Fiscal Year Final Research Report
Development of innovative mutation test
| Project/Area Number |
22651021
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 突然変異 / SMRT DNAシーケンサー / 変異原性試験 |
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| Research Abstract |
I proposed here an anticipated mutation assay which estimates the frequency and spectrum of somatic point mutations in any genes of any samples by using SMRT.(Single Molecule Real Time) DNA sequencing technology. The basic concept of the mutation assay is very simple : just prepare the target template and sequence it accurately. In this project, I considered ideas on how to make the template and how to eliminate artifactual mutations caused by damage to the DNA template. On the other hands, I developed a genome-wide mutation assay using Illumina next-generation DNA sequencer.
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