2011 Fiscal Year Final Research Report
Creation of biocatalyst capable of biosynthesizing lactate-based polymers with highly regulated chirality
| Project/Area Number |
22651032
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAGUCHI Seiichi 北海道大学, 大学院・工学研究院, 教授 (70216828)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 生分解性プラスチック / 進化工学 / ポリヒドロキシアルカン酸 / キラリティー / 光学純度 / 乳酸ポリマー / 重合酵素 / モノマー供給系酵素 |
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| Research Abstract |
Currently microbial production system for lactate(LA) polymers with high performances has been developed. A break-through of this system was a discovery of "LA-Polymerizing Enzyme(LPE)". LPE was created through the study on the engineering of microbial polymer PHA synthase. LA-based polymer was synthesized by highly enatiomeric monomers including R-LA in an one-pot manner in Escherichia coli by introduction of the LPE gene from glucose. I tried to establish the system for synthesis of the polymer incorporating S-LA monomer based on the evolutionary engineering of CoA-transferase and LPE. As a resiult, LA fraction in the polymer has been enriched up to 47% from 6% by combination of metabolic and enzyme engineering. Furthermore, LA-based polymers exhibited distinguishable properties from the counterpart homopolymers, PLA and PHB.
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