Gene activating RNAs based on naturally occuring promoter associated RNAs

2011 Fiscal Year Final Research Report

• Project/Area Number: 22651077
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: System genome science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: FORREST Alistair 独立行政法人理化学研究所, ゲノムプロファイル技術開発ユニット, ユニットリーダー (40569084)
• Project Period (FY): 2010 – 2011
• Keywords: リボ核酸 / 遺伝子発現を / プロモータ

Research Abstract

The research project was funded by a 'challenging exploratory' research grant. The project aimed to search for design rules for inducing gene up-regulation by synthetic short RNA molecules that target the promoter region of each gene. Synthetic RNAs were designed to target upstream antisense transcripts. In total 25 genes were tested in 2 cell lines. Four potential short activating RNAs were identified for EGR2, NANOG, SPI1, MAFB, however the effect was small and did not work in both cell types. Unfortunately the experiments did not result in any publications.