Development of reverse-genetics methods for Daphnids

2011 Fiscal Year Final Research Report

• Project/Area Number: 22651078
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied genomics
• Research Institution: National Institutes of Natural Sciences Okazaki Research Facilities
• Principal Investigator: IGUCHI Taisen 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (90128588)
• Project Period (FY): 2010 – 2011
• Keywords: ミジンコ / 逆遺伝学 / エコゲノミクス

Research Abstract

The water flea Daphnia magna is a small crustacean which is an ubiquitous inhabitant in fresh water. As daphnids inhabit a diverse array of aquatic environments, they are a good model for understanding response and adaptation to environmental changes and evolution. Therefore, they have been used one of standard organisms in ecotoxicology. While daphnids have significant importance in ecotoxicology, tools to understand response to environmental changes in daphnia is very limited. We investigate a condition for natural male production and improve introduction of foreign DNA into daphnids. These basic information will be available for establishment of transgenic daphnids.

Research Products

• [Journal Article] Environmental sex determination in the branchiopod crustacean Daphnia magna : Deep conservation of a Doublesex gene in the sex-determining pathway (2011)
  • Author(s): Kato, Y., Kobayashi, K., Watanabe, H. and Iguchi, T
  • Journal Title: PLoS Genetics Volume: 7 Pages: e1001345
  • Peer Reviewed
• [Journal Article] Development of a RNA interference method in the cladoceran crustacean Daphnia magna (2011)
  • Author(s): Kato, Y., Shiga, Y., Kobayashi, K., Tokishita, S., Yamagata, H., Iguchi, T. and Watanabe, H
  • Journal Title: Devel. Genes Evol Volume: 220 Pages: 337-345
  • Peer Reviewed
• [Journal Article] Morphological changes in Daphnia galeata induced by a crustacean terpenoid hormone and its analog (2011)
  • Author(s): Oda, S., Kato, Y., Watanabe, H., Tatarazako, N., Iguchi, T
  • Journal Title: Environ Toxicol Chem Volume: 30 Pages: 232-238
  • Peer Reviewed
• [Journal Article] Demasculinization and feminization of male gonads by atrazine : consistent effects across vertebrate classes (2011)
  • Author(s): Hayes, T. B., Beasley, V. R., de Solla, S., Iguchi, T., et al
  • Journal Title: J. Steroid Biochem Mol. Biol Volume: 127 Pages: 64-73
  • Peer Reviewed
• [Journal Article] Endocrine disrupting chemicals. J. Steroid Biochem (2011)
  • Author(s): Blumberg, B., Iguchi, T. and Odermatt, A
  • Journal Title: Mol. Biol Volume: 127 Pages: 1-3
• [Journal Article] Species extrapolation for the 21st century. Environ (2011)
  • Author(s): Celander, M. C., Goldstone, J. V., Denslow, N. D., Iguchi, T., Kille, P., Meyerhoff, R. D., Smith, B. A., Hutchinson T. H. and Wheeler, J. R
  • Journal Title: Toxicol. Chem Volume: 30 Pages: 5263
  • Peer Reviewed
• [Journal Article] Sequence divergence and expression of a transformer gene in the branchiopod crustacean (2010)
  • Author(s): Kato, Y., Kobayashi, K., Oda, S., Tatarazako, N., Watanabe, H. and Iguchi, T
  • Journal Title: Daphnia magna. Genomics Volume: 95 Pages: 160-165
  • Peer Reviewed
• [Journal Article] Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation (2010)
  • Author(s): Kato, Y., Kobayashi, K., Watanabe, H. and Iguchi, T
  • Journal Title: Ecotoxicology Volume: 19 Pages: 589-592
  • Peer Reviewed
• [Presentation] 内分泌かく乱物質の生物影響に関する研究 (2011)
  • Author(s): 井口泰泉
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 北海道旭川市
  • Year and Date: 20110921-23
• [Presentation] Endocrine disrupters issues in Japan (2011)
  • Author(s): Iguchi, T
  • Organizer: 2nd International Conference on Endocrine Disruptors
  • Place of Presentation: ドイツ
  • Year and Date: 20110607-08
• [Presentation] Endocrine disruption in invertebrates : Sex determination of Daphnia magna (2011)
  • Author(s): Iguchi, T
  • Organizer: 6th Copenhagen Workshop on Endocrine Disrupters
  • Place of Presentation: デンマーク
  • Year and Date: 20110426-29
• [Presentation] Endocrine disruption in invertebrates : sex determination of Daphnia magna (2010)
  • Author(s): Iguchi, T
  • Organizer: 11^<th> e. hormone Symposium
  • Place of Presentation: アメリカ
  • Year and Date: 2010-10-19
• [Remarks]
  • URL: http://www.nibb.ac.jp/%7Ebioenv1/index-j.html