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2011 Fiscal Year Final Research Report

Thermotolerance through isoprene emission and the novel mechanism of decrease in leaf surface temperature in plants

Research Project

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Project/Area Number 22657014
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto University

Principal Investigator

YAZAKI Kazufumi  京都大学, 生存圏研究所, 教授 (00191099)

Project Period (FY) 2010 – 2011
Keywords環境応答 / イソプレン / 高温耐性 / プラスチド / MEP経路 / 揮発性有機化合物
Research Abstract

Mechanism of thermotolerance by expressing isoprene synthase(IspS) in Arabidopsis thaliana has been studied, in which transgenic plants were treated at 60℃for 2. 5 hr. We compared various endogenous plant hormone contents between wild type and IspS-expressing Arabidopsis systematically, but could find significant difference. During the analysis we found that the tolerance to drastic and short time heat shock seems to be different to recovery rate of treated plants on cultivation at 23℃for a week. Considering with a recent report on the tolerance of IspS-expressing tobacco plant to ozone stress, it is suggested that the thermotolerance we observed may be due to the depletion of reactive oxygen species raised after heat shock treatment in plastids by isoprene, which actually functions as OH radical scavenger in atomosphere.

  • Research Products

    (5 results)

All 2011 2010 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Molecular characterization of a membrane-bound prenyltransferase specific for isoflavone from Sophora flavescens2011

    • Author(s)
      Sasaki, K., Tsurumaru, Y., Yamamoto, H., Yazaki, K.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 24125-24134

    • DOI

      DOI:10.1074/jbc.M111.244426

    • Peer Reviewed
  • [Journal Article] A Tolerance gene for prenylated flavonoid encodes 26S proteasome regulatory subunit in Sophora flavescens2011

    • Author(s)
      Shitan, N., Yazaki, K.
    • Journal Title

      Biosci. Biotech. Biochem.

      Volume: 75 Pages: 982-984

    • DOI

      10.1271/bbb.100665

    • Peer Reviewed
  • [Presentation] 植物のイソプレノイド単位DMAPPを巡る分泌型低分子の代謝生理2011

    • Author(s)
      矢崎一史
    • Organizer
      科学技術交流財団研究会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-12-22
  • [Presentation] イソプレン放出による高温耐性機構の解析2010

    • Author(s)
      馬場奈央登、佐々木佳菜子、矢崎一史
    • Organizer
      第28回日本植物細胞分子生物学会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-03
  • [Remarks]

    • URL

      http://www.rish.kyoto-u.ac.jp/W/LPGE/

URL: 

Published: 2013-07-31  

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