2011 Fiscal Year Final Research Report
Establishment of novel reaction mechanism on glycosylase and its application
| Project/Area Number |
22658032
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIMURA Atsuo 北海道大学, 大学院・農学研究院, 教授 (90186312)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 反応機構 / 糖質分解酵素 / 反応中間体 |
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| Research Abstract |
Two reaction mechanisms have been proposed : on glycosylases, i. e. oxo-carbenium ion-mediated mechanism and covalent linkage-formation mechanism. Recently, the latter one is supported by many glycosylase-scientists. However, there is a problem in the latter mechanism : this mechanism cannot be supported by organic chemistry-related knowledge. A purpose of this project is an experimental proof of the oxo-carbenium ion-mediated mechanism. Glycosylases catalyze the useful reaction of transglycosylation. This reaction mechanism may produce the novel application approach, the possibility of which is also investigated.
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