2011 Fiscal Year Final Research Report
A novel strategy for the generation of anti-glycan antibodies and its application
| Project/Area Number |
22659018
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | リンパ球ホーミング / L-セレクチン / 高内皮細静脈 / 硫酸化糖鎖 / 抗糖鎖抗体 |
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| Research Abstract |
Anti-carbohydrate monoclonal antibodies(mAbs) are very useful in the functional analysis of complex carbohydrates in vivo. However, such mAbs are difficult to generate, largely because a wide variety of complex carbohydrates is intrinsically expressed in mice and rats and because the antigenicities of glycans are generally poor. In this study, we developed an efficient method for generating anti-carbohydrate mAbs using glycan-synthesizing enzyme-knockout mice in which the glycan structures formed by the missing enzymes should be highly antigenic. As an application of this method, we generated anti-sulfated glycan mAbs using sulfotransferase-deficient mice and found that sulfated N-and sulfated O-glycans function cooperatively in lymphocyte homing and immune surveillance using those mAbs.
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Research Products
(22 results)
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[Journal Article] Endothelial Heparan Sulfate Controls Chemokine Presentation in Recruitment of Lymphocytes and Dendritic Cells to Lymph Nodes2010
Author(s)
Bao X, Moseman EA, Saito H, Petryanik B, Thiriot A, Hatakeyama S, Ito Y, Kawashima H, Yamaguchi Y, Lowe JB, von Andrian UH and Fukuda M
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Journal Title
Immunity
Volume: 33
Pages: 817-829
DOI
Peer Reviewed
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[Presentation] Novel Anti-Carbohydrate Antibodies Reveal the Cooperative Function of Sulfated N-and O-Glycans in Lymphocyte Homing2011
Author(s)
Hirakawa J, Tsuboi K, Sato K, Kobayashi M, Watanabe S, Takakura A, Imai Y, Ito Y, Fukuda M, and Kawashima H
Organizer
Keystone Symposia-New Frontiers at the Interface of Immunity and Glycobiology
Place of Presentation
Lake Louise, Alberta, Canada
Year and Date
2011-03-08
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