2011 Fiscal Year Final Research Report
adductome analysis of human tissues
Project/Area Number |
22659072
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Human pathology
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Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
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Project Period (FY) |
2010 – 2011
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Keywords | DNA付加体 / adductome / 炎症 / 脂質過酸化物 / 胃がん / 塩基除去修復LC-MS / ゲノム |
Research Abstract |
High performance liquid chromatography and tandem mass spectrometry disclosed at least 10 lipid peroxidation related modified DNA bases in the DNAs extracted under anti-oxidation condition from human autopsy tissues. Among these 10 DNA adducts, 7 of them, 1, N6-etheno-2'-deoxyadenosine(εdA), butanone-etheno-2'-deoxycytidine(BεdC), butanone-etheno-2'-deoxy-5-methylcytidine(BεmedC), butanoneetheno-2'-deoxyadenosine(BεdA), heptanone-etheno-2'-deoxycytidine(HεdC), heptanone-etheno-2'-deoxyadenosine(HεdA), and heptanone-etheno-2'-deoxyguanosine(HεdG) were quantitated in gastric mucosa, resected for gastric cancer in Japan and China. The profiles of these DNA adducts identified the origin of the tissues(China or Japan), implying adductome profile may reflect environmental exposures of individual settings. We further tested the repair machinery against these modified bases and discovered TDG play s role in repairing some of these adducts.
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Research Products
(10 results)
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[Journal Article] Germline alterations of the CDH1 gene in familial gastric cancer in the Japanese population2011
Author(s)
Yamada, H., Shinmura, K., Ito, H., Kasami, M., Sasaki, N., Shima, H., Ikeda, M., Tao, H., Goto, M., Ozawa, T., Tsuneyoshi, T., Tanioka, F., and Sugimura
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Journal Title
Cancer Sci
Volume: 103
Pages: 1782-1788
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[Journal Article] Novel Single Nucleotide Polymorphism of the CYP2A13 gene in Japanese individuals2011
Author(s)
Tamaki, Y., Honda, M., Muroi, Y., Arai, T., Sugimura, H., Matsubara, Y., Kanno, S., Ishikawa, M., Hirasawa, N., and Hiratsuka, M.
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Journal Title
Drug Metab Pharmacokinet
Volume: 26(5)
Pages: 544-547
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[Journal Article] Detection of lipid peroxidation-induced DNA adducts caused by 4-oxo-2(E)-nonenal and 4-oxo-2(E)-hexenal in human autopsy tissues2010
Author(s)
Chou, P. H., Kageyama, S., Matsuda, S., Kanemoto, K., Sasada, Y., Oka, M., Shinmura, K., Mori, H., Kawai, K., Kasai, H., Sugimura, H., and Matsuda, T.
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Journal Title
Chem Res Toxicol
Volume: 23(9)
Pages: 1442-8
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[Journal Article] Adenine DNA glycosylase activity of 14 Human MutY homolog(MUTYH) variant proteins found in patients with colorectal polyposis and cancer2010
Author(s)
Goto, M., Shinmura, K., Nakabeppu, Y., Tao, H., Yamada, H., Tsuneyoshi, T., and Sugimura, H.
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Journal Title
Hum Mutat
Volume: 31
Pages: E1881-74
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[Journal Article] Identification of 5 novel germline APC mutations and characterization of clinical phenotypes in Japanese patients with classical and attenuated familial adenomatous polyposis2010
Author(s)
Tao, H., Shinmura, K., Yamada, H., Maekawa, M., Osawa, S., Takayanagi, Y., Okamoto, K., Terai, T., Mori, H., Nakamura, T., and Sugimura, H.
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Journal Title
BMC Res Notes
Volume: 3(1)
Pages: 305
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