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2012 Fiscal Year Final Research Report

Analysis of 3-D cultured dental pulp cells and osteoblasts and their clinical application

Research Project

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Project/Area Number 22659343
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KAWASHIMA Nobuyuki  東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (60272605)

Project Period (FY) 2010 – 2012
Keywords歯髄細胞 / 骨芽細胞 / 象牙芽細胞 / 細胞分化 / 硬組織形成 / 三次元培養
Research Abstract

Dental pulp cells are differentiate into odontoblasts, which are dentin-forming cells, and osteoblasts are bone tissue-forming cells. These cells are categorized as hard tissue forming cells, and may possess common properties. Mineralization of these cells in the conventional two dimensional culture is only observed in the presence of mineralization-inducing factors, such as BMPs or ascorbic acid and beta-glycerophosphate. We revealed that three dimensional (3-D) culture induced up-regulation of odonto-/osteo-blast marker expression in dental pulp cells and osteoblasts. Transplantation of 3-D cultured pulp cells or osteoblasts into bone cavities effectively induced hard tissue formation. Clinical application of 3-D cultured dental pulp cells or osteoblasts are expected.

  • Research Products

    (32 results)

All 2013 2012 2011 2010

All Journal Article (9 results) Presentation (23 results)

  • [Journal Article] Kinetics of Th17-related cytokine expression in experimentally induced rat periapical lesions2013

    • Author(s)
      S Wei, N Kawashima, N Suzuki, J Xu, S Takahashi, M Zhou, Y Koizumi, H Suda
    • Journal Title

      Aust Endod J

      Volume: (in press)

    • DOI

      doi:10.1111/j.1747-4477.2012.00371.x

  • [Journal Article] A new horizon for tissue regeneration?2012

    • Author(s)
      Kawashima N. Characterisation of dental pulp stem cells
    • Journal Title

      Arch Oral Biol.

      Volume: 57 Pages: 1439-58

    • DOI

      doi:10.1016/j.archoralbio.2012.08.010

  • [Journal Article] Promotional effects of vasoactive intestinal peptide on the development of rodent Hertwig's epithelial root sheath2012

    • Author(s)
      J Xu, N Kawashima, N Fujiwara, H Harada, MS. Ota, H Suda
    • Journal Title

      Congenital Anomalies

      Volume: Volume 52, Issue 3 Pages: 162-167

    • DOI

      doi:10.1111/j.1741-4520.2012.00371.x.

  • [Journal Article] Comparison of gingiva, dental pulp, and periodontal ligament cells from the standpoint of mesenchymal stem cellproperties2012

    • Author(s)
      Koji Otabe, Takeshi Muneta, Nobuyuki Kawashima, Hideaki Suda, Kunikazu Tsuji, Ichiro Sekiya
    • Journal Title

      Cell Medicine

      Volume: Vol. 4 Pages: 13-21

  • [Journal Article] Requirement of Integrin s3 for IronTransportation during Enamel Formation2012

    • Author(s)
      T. Yoshida, Y. Kumashiro, T. Iwata, J. Ishihara, T. Umemoto, Y. Shiratsuchi, N. Kawashima, T. Sugiyama, M. Yamato and T. Okano
    • Journal Title

      J DENT RES published online

  • [Journal Article] MEPE Activated by Furin Promotes Pulpal Cell Adhesion2011

    • Author(s)
      H.G. Wang, N. Kawashima, T. Iwata, J. Xu, S. Takahashi, T. Sugiyama, H. Suda
    • Journal Title

      Jouranal of Dental Research

      Volume: 90(4) Pages: 529-534

    • DOI

      doi:10.1177/0022034510391057

  • [Journal Article] Osteogenic differentiation of mouse mesenchymal progenitor cell, Kusa-A1 is promoted by mammalian transcriptional repressor2010

    • Author(s)
      S. Wang, N. Kawashima, K. Sakamoto, K. Katsube, A. Umezawa, H Suda
    • Journal Title

      Rbpj Biochemical and Biophysical Research Communications

      Volume: 400 Pages: 39-45

    • URL

      http://dx.doi.org/10.1016/j.bbrc.2010.07.133

  • [Journal Article] Differentiation of Odontoblasts Is Negatively Regulated by MEPE via Its C-Terminal Fragment2010

    • Author(s)
      H.G. Wang, N. Kawashima , T. Iwata, J. Xu, S. Takahashi, T. Sugiyama, H. Suda
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 398 Pages: 406-12

    • URL

      http://dx.doi.org/10.1016/j.bbrc.2010.06.085

  • [Journal Article] Expression of Notch-Signalling-related Genes in Normal and Differentiating Rat Dental Pulp Cells2010

    • Author(s)
      Sun H, Kawashima N, Xu J, TakahashiS, Suda H
    • Journal Title

      Austrarian Endodontic Journal

      Volume: 36 Pages: 54-8

    • DOI

      doi:10.1111/j.1747-4477.2009.00188.x

  • [Presentation] Induction of Mineralization by Spheroid Cultured-Dental Pulp Stem Cells2012

    • Author(s)
      KAWASHIMA Nobuyuki, YAMAMOTO Mioko, SAITO Masahiro, TAKIMOTO Koyo, ZHOU Mengyu, KOIZUMI Yu, SUZUKI Noriyuki and SUDA Hideaki
    • Organizer
      日本歯科保存学会秋季大会(イングリッシュセッション)
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2012-11-22
  • [Presentation] Osteoblast differentiation was negativelyregulated by periostin2012

    • Author(s)
      Kawashima N, Mengyu Z, Katsube K, Kudo A, Suda H
    • Organizer
      IADR 90th General Session
    • Place of Presentation
      Iguacu Falls, Brazil
    • Year and Date
      2012-06-20
  • [Presentation] Effects of 3-D Spheroid Culture on DentalPulp Cells2012

    • Author(s)
      M. YAMAMOTO, N. KAWASHIMA, Y. KOIZUMI, K. TAKIMOTO, M. SAITO, H. HARADA, and H. SUDA
    • Organizer
      IADR 90th General Session
    • Place of Presentation
      Iguacu Falls, Brazil
    • Year and Date
      2012-06-20
  • [Presentation] Effects of MMP-3 on Mediator Synthesis and Accumulation of Inflammatory Cells2012

    • Author(s)
      K. Takimoto, N. Kawashima, N. Suzuki, M. Nakashima, H. Suda
    • Organizer
      AAE 2012 Annual Session
    • Place of Presentation
      Boston, MA
    • Year and Date
      2012-04-20
  • [Presentation] Involvement of a Myogenic TranscriptionalFactor in Odontoblast Differentiation2012

    • Author(s)
      N. Kawashima, J. Xu, N. Suzuki, M. Zhou, K. Takimoto, Y. Koizumi, M. Yamamoto, H. Suda
    • Organizer
      AAE 2012 Annual Session
    • Place of Presentation
      Boston, MA
    • Year and Date
      2012-04-20
  • [Presentation] Regulation of Periapical Bone Destructionby Cathepsin K Inhibitor2012

    • Author(s)
      N. Suzuki, N. Kawashima, K. Takimoto, H. Suda
    • Organizer
      AAE 2012 Annual Session
    • Place of Presentation
      Boston, MA
    • Year and Date
      2012-04-20
  • [Presentation] Mechanisms of Bone Destruction and its Regulation in the Periapical Lesions2011

    • Author(s)
      Suzuki N, Kawashima N, Suda H
    • Organizer
      KACD
    • Place of Presentation
      Kim Koo Museum, Seoul.
    • Year and Date
      20111111-12
  • [Presentation] Down-regulation of Inflammatory Mediator Synthesis from macrophages by MMP32011

    • Author(s)
      N Kawashima, K Takimoto, Y Koizumi, M Yamamoto, M Zhou, N Suzuki, M Nakashima, H Suda
    • Organizer
      KACD
    • Place of Presentation
      Kim Koo Museum, Seoul.
    • Year and Date
      20111111-12
  • [Presentation] Shh and Wnt Signaling in AmeloblastDifferentiation2011

    • Author(s)
      S Takahashi, N Kawashima, K Katsube, T Sugiyama, H Sida
    • Organizer
      ESE
    • Place of Presentation
      CavalieriHotel, Rome.
    • Year and Date
      20110915-17
  • [Presentation] Mineralization of Odonotoblastic-Lineage Cells Was Accelerated by Enhanced Expression of Mef2c2011

    • Author(s)
      N Kawashima, J Xu, N Suzuki, M Zhou, K Takimoto, Y Koizumi, M Yamamoto, S Takahashi, T Sugiyama, H Suda
    • Organizer
      ESE
    • Place of Presentation
      CavalieriHotel, Rome.
    • Year and Date
      20110915-17
  • [Presentation] 単層培養法と三次元培養法における歯髄細胞の象牙芽細胞分化について2011

    • Author(s)
      山本弥生子、川島伸之、須田英明
    • Organizer
      日本歯科保存学会秋季大会(第135回)
    • Place of Presentation
      大阪国際交流センター
    • Year and Date
      2011-10-20
  • [Presentation] ペリオスチンによる骨芽細胞分化制御2011

    • Author(s)
      川島伸之、周夢宇、須田英明、工藤明、勝部憲一
    • Organizer
      日本歯科保存学会秋季大会(第135回)
    • Place of Presentation
      大阪国際交流センター
    • Year and Date
      2011-10-20
  • [Presentation] Mechanisms of Bone Destruction in the Periapical Lesions and Its Regulation2011

    • Author(s)
      Kawashima N, Suzuki N, Suda H
    • Organizer
      The16th Scientific Meeting of Asian Pacific Endodontic Confederation
    • Place of Presentation
      Jahad Daneshgahi Complex, Shiraz, Iran
    • Year and Date
      2011-04-21
  • [Presentation] Expression of Mef2c in Murine Odontoblsts2010

    • Author(s)
      N Kawashima, J Xu, N Suzuki, M Zhou, K Takimoto, Y Koizumi, M Yamamoto, A Salima, S Takahashi, H Suda
    • Organizer
      8th World Endodontic Congress
    • Place of Presentation
      in Athens, Greece.
    • Year and Date
      20101006-09
  • [Presentation] Shh Signaling is Related to BMP and WntSignaling in Ameloblast Differentiation2010

    • Author(s)
      S Takahashi, N Kawashima, K Katsube, T Sugiyama, H Sida
    • Organizer
      8th World Endodontic Congress
    • Place of Presentation
      in Athens, Greece
    • Year and Date
      20101006-09
  • [Presentation] Wnt Signaling in Odontoblast Differentiation Induced by BMP2010

    • Author(s)
      Y Koizumi, N Kawashima, K Takimoto, S Takahashi, J Xu, N Suzuki, M Zhou, M Yamamoto, A Salima, T Sugiyama, H Suda
    • Organizer
      8th World Endodontic Congress
    • Place of Presentation
      in Athens, Greece
    • Year and Date
      20101006-09
  • [Presentation] Analysis of osteoblastic mesenchymal cell adhesion on laser-induceddentin modification2010

    • Author(s)
      Gombo Bolortuya, Arata Ebihara, Satoshi Watanabe, Tomoo Anjo, Chizuko Kokuzawa, Hidetoshi Saegusa, Nobuyuki Kawashima, and Hideaki Suda
    • Organizer
      8th World Endodontic Congress
    • Place of Presentation
      in Athens, Greece
    • Year and Date
      20101006-09
  • [Presentation] Promotive effects of Sp7 on Dsppexpression2010

    • Author(s)
      N Kawashima, J Xu, T Iwata, S Takahashi, Y Koizumi, K Takimoto, Z Mengyu, C Ohi, N Suzuki, T Sugiyama, H Suda
    • Organizer
      89th IADR General Session & Exhibition
    • Place of Presentation
      in Barcelona, Spain
    • Year and Date
      20100714-17
  • [Presentation] Effects of MMP-3 on InflammatoryMediator Synthesis from Macrophages2010

    • Author(s)
      K Takimoto, N Kawashima, M Nakashima, H Nakamura, Y Koizumi, and H Suda
    • Organizer
      89th IADR General Session & Exhibition
    • Place of Presentation
      in Barcelona, Spain
    • Year and Date
      20100714-17
  • [Presentation] Effective suppression of periapical lesion expansion by Cathepsin K inhibitor2010

    • Author(s)
      Suzuki N, Kawashima N, Xu J, Suda H.
    • Organizer
      89th IADR General Session & Exhibition
    • Place of Presentation
      in Barcelona, Spain
    • Year and Date
      20100714-17
  • [Presentation] Expression of Vasoactive Intestinal PeptideReceptors in HERS Cells2010

    • Author(s)
      J Xu, N Kawashima, N Fujiwara, H Harada, H Suda
    • Organizer
      89th IADR General Session & Exhibition
    • Place of Presentation
      in Barcelona, Spain
    • Year and Date
      20100714-17
  • [Presentation] Induction of IL17 synthesis in the experimentally-induced murine periapical lesions2010

    • Author(s)
      Kawashima N., Xu J., Suzuki N., Zhou M., Wei S., Takimoto K., Koizumi Y., Takahashi S., Suda H.
    • Organizer
      2010 Spring Scientific Meeting of KoreanAcademy of Endodontics
    • Place of Presentation
      BEXCO Busan, Korea
    • Year and Date
      2010-03-27
  • [Presentation] 須田英明象牙芽細胞分化における Sp7 による dentin sialophosphoprotein 発現誘導2010

    • Author(s)
      川島伸之、許セイ、岩田隆紀、周夢宇、瀧本晃陽、小泉悠、大井智恵、高橋里美、鈴木規元
    • Organizer
      日本再生医療学会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2010-03-18

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Published: 2014-08-29  

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