Functional analysis of DNA damage response of transcription factor FOXO1 in lifespan regulation

2012 Fiscal Year Final Research Report

• Project/Area Number: 22688029
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: University of Tsukuba
• Principal Investigator: DAITOKU Hiroaki 筑波大学, 生命環境系, 講師 (30361314)
• Project Period (FY): 2010 – 2012
• Keywords: 老化 / 転写因子 / 紫外線 / DNA損傷修復

Research Abstract

Transcription factor FOXO1 is known to be involved in lifespan extension and anti-aging; however, the molecular mechanism remains unclear. In this study, we found that FOXO1 contributes to tlanslesion DNA synthesis, which serves as the DNA damage response against ultra violet (UV) irradiation. Furthermore, genetic analysis using C. elegans showed that FOXO1/DAF-16 plays a crucial role in UV resistance during larval development. Our finding will help to elucidate the anti-aging mechanism of FOXO1.

Research Products

• [Journal Article] GSK3β regulates gluconeogenic gene expression through HNF4α and FOXO1J.Recept (2012)
  • Author(s): Sakamaki JI, Daitoku H, Kaneko Y, Hagiwara A, Ueno K, Fukamizu A
  • Journal Title: Signal Transduct Volume: 32 Pages: 96-1011
  • DOI: DOI:10.3109/10799893.2012.660531
  • Peer Reviewed
• [Journal Article] Asymmetric arginine dimethylation determines life span in C. elegans by regulating forkhead transcription factor DAF-16 (2011)
  • Author(s): Takahashi Y, Daitoku H, Hirota K, Tamiya H, Yokoyama A, Kako K, Nagashima Y, Nakamura A, Shimada, T, Watanabe S, Yamagata K, Yasuda K, Ishii N, Fukamizu A
  • Journal Title: Cell Metab Volume: 13 Pages: 505-516
  • DOI: DOI:10.1016/j.cmet.2011.03.017
  • Peer Reviewed
• [Journal Article] Arginine methylation of BCL-2 antagonist of cell death (BAD) counteracts its phosphorylation and inactivation by Akt (2011)
  • Author(s): Sakamaki JI, Daitoku H, Ueno K, Hagiwara A, Yamagata K, Fukamizu A
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 6085-6090
  • DOI: DOI:10.1073/pnas.1015328108
  • Peer Reviewed
• [Journal Article] Regulation of FoxO transcription factors by acetylation and protein-protein interactionsBiochem. Biophys (2011)
  • Author(s): Daitoku H, Sakamaki JI,Fukamizu A
  • Journal Title: Biochem. Biophys. Acta Volume: 1813 Pages: 1954-1960
  • DOI: DOI:10.1016/j.bbamcr.2011.03.001
  • Peer Reviewed
• [Journal Article] The C. elegans PRMT-3 possesses a type III protein arginine methyltransferases activity (2011)
  • Author(s): Takahashi Y, Daitoku H, Yokoyama A, Nakayama K, Kim JD, Fukamizu A
  • Journal Title: J.Recept. Signal Transduct Volume: 31 Pages: 168-172
  • DOI: DOI:10.3109/10799893.2011.555768
  • Peer Reviewed
• [Presentation] 転写因子FOXO1は紫外線DNA損傷応答に関与する (2013)
  • Author(s): 金子悠太、大徳浩照、深水昭吉
  • Organizer: 若手ワークショップ@鬼怒川
  • Place of Presentation: 栃木県日光市
  • Year and Date: 2013-01-24
• [Presentation] 転写因子Foxo1は紫外線DNA損傷応答に関与する (2012)
  • Author(s): 金子悠太、大徳浩照、深水昭吉
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
• [Presentation] 転写因子Foxo1は紫外線DNA損傷応答に関与する (2011)
  • Author(s): 金子悠太、大徳浩照、深水昭吉
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] フォークヘッド転写因子DAF-16は線虫の損傷乗り越え複製に関与する (2011)
  • Author(s): 松本佳保里、大徳浩照、深水昭吉
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14
• [Remarks]
  • URL: http://akif2.tara.tsukuba.ac.jp