Roles of gap junction proteins in odontoblast differentiation

2013 Fiscal Year Final Research Report

• Project/Area Number: 22689053
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: The University of Tokushima (2013); Tohoku University (2010-2012)
• Principal Investigator: IWAMOTO Tsutomu 徳島大学, ヘルスバイオサイエンス研究部, 教授 (90346916)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: 歯胚発生 / 象牙芽細胞 / 細胞間結合 / パネキシン3

Research Abstract

In this project, we had performed bioinformatical analysis to find the responsible gene which regulate odontoblast differentiation. We had found that pannexin3, a new member of gap junction protein, pannexin family, was preferentially expressed in tooth cDNA library. In fact, in situ hybridization and immunostaining revealed that pannexin3 was expressed in preoodontoblast layer. To analyze the function of pannexin3 in odontoblast, mDP cell, a mouse dental papilla derived cell line, was used in this study. We found that pannexin3 regulate cell proliferation and differentiation by releasing intracellular ATP and activating AMPK signaling.

Research Products

• [Journal Article] Pannexin 3 inhibits proliferation of osteoprogenitor cells by regulating Wnt and p21 signaling (2014)
  • Author(s): Ishikawa M, Iwamoto T, Fukumoto S, Yamada Y
  • Journal Title: J Biol Chem Volume: 289(5) Pages: 2839-2851
  • DOI: 10.1074/jbc.M113.523241
  • Peer Reviewed
• [Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation (2012)
  • Author(s): Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
  • Journal Title: J Biol Chem Volume: 287(13) Pages: 10590-10601
  • DOI: 10.1074/jbc.M111.285874
  • Peer Reviewed
• [Journal Article] Glycosphingolipids regulate ameloblastin expression in dental epithelial cells (2012)
  • Author(s): Kamasaki Y, Nakamura T, Yoshizaki K, Iwamoto T, Yamada A, Fukumoto E, Maruya Y, Iwabuchi K, Furukawa K, Fujiwara T, Fukumoto S
  • Journal Title: J. Dent. Res Volume: 91(1) Pages: 78-83
  • DOI: 10.1177/0022034511424408
  • Peer Reviewed
• [Journal Article] Pannexin 3 functions as an ER Ca2+ channel, hemichannel, and gap junction to promote osteoblast differentiation (2011)
  • Author(s): Ishikawa M, Iwamoto T, Nakamura T, Doyle A, Fukumoto S, Yamada Y
  • Journal Title: J Cell Biol Volume: 27; 193(7) Pages: 1257-1274
  • DOI: 10.10.83/jcb.201101050
  • Peer Reviewed
• [Journal Article] Pannexin 3 regulates intracellular ATP/cAMP levels and promotes chondrocyte differentiation (2010)
  • Author(s): Iwamoto T, Nakamura T, Doyle A, Ishikawa M, de Vega S, Fukumoto S, Yamada Y
  • Journal Title: J Biol Chem Volume: 285(24) Pages: 18948-18958
  • DOI: 10.1074/jbc.M110.127027
  • Peer Reviewed
• [Journal Article] PDGFs regulate tooth germ proliferation and ameloblast differentiation (2010)
  • Author(s): Nun W, Iwamoto T, Sugawara Y, Futaki M, Yoshizaki K, Yamamoto S, Yamada A, Nakamura T, Nonaka K, and Fukumoto S
  • Journal Title: Archives of Oral Biology Volume: 55(6) Pages: 426-434
  • DOI: 10.1016/j.archoralbio
  • Peer Reviewed
• [Presentation] 歯から全身へー歯髄幹細胞の可能性- (2013)
  • Author(s): 岩本 勉
  • Organizer: 第32回日本小児歯科学会中四国地方会
  • Place of Presentation: 岡山大学創立五十周年記念館(岡山県)
  • Year and Date: 2013-11-24
• [Presentation] ヒト乳歯歯髄細胞におけるSDF-1の発現調節機構について (2013)
  • Author(s): 長谷川智一, 赤澤友基, 帖佐直幸, 吉村善隆, 浅川剛吉, 石崎 明, 岩本 勉
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山コンベンションセンター(岡山県)
  • Year and Date: 2013-09-22
• [Presentation] 骨・軟骨形成過程におけるギャップ結合分子パネキシン3の機能解析 (2013)
  • Author(s): 岩本 勉
  • Organizer: 第55回歯科基礎医学会学術大会・総会サテライトシンポジウム
  • Place of Presentation: 岡山コンベンションセンター(岡山県)
  • Year and Date: 2013-09-20
• [Presentation] 唾液腺発生過程におけるConnexin43-FGF10 シグナルの解析 (2013)
  • Author(s): 福本 敏, 二木正晴, 岩本 勉, 中村卓史, 山田亜矢
  • Organizer: 第51回日本小児歯科学会大会
  • Place of Presentation: 長良川国際会議場(長野県)
  • Year and Date: 2013-05-23
• [Presentation] Pannexin 3, a gap junction protein, regulates odontoblast differentiation (2012)
  • Author(s): Iwamoto T, Ishikawa M, Yoshizaki K, Yamada A, Nakamura T, Yamada Y, Fukumoto S
  • Organizer: The 90th General Session & Exhibition of the International Association of Dental Research (IADR)
  • Place of Presentation: Iguacu Falls Convention Center(イグアスフォールズ, ブラジル)
  • Year and Date: 2012-06-22
• [Presentation] エナメル芽細胞分化過程におけるテトラスパニンCD9の発現とその役割 (2012)
  • Author(s): 岩本 勉, 小野真理子, 二木正晴, 菅原優, 山田亜矢, 中村卓史, 福本 敏
  • Organizer: 第50回日本小児歯科学会大会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2012-05-12
• [Presentation] Pannexin 3, a gap junction protein, regulates proliferation through AMPK pathway in odontoblasts (2012)
  • Author(s): Iwamoto T, Ishikawa M, Yoshizaki K, Nakamura T, Yamada A, Yamada Y, Fukumoto S
  • Organizer: 2012 American society for Biochemistry and Molecular Biology Annual meeting
  • Place of Presentation: San Diego Convention Center, USA(サンディエゴ, 米国)
  • Year and Date: 2012-04-25
• [Presentation] 象牙芽細胞分化制御機構の同定とその応用 (2011)
  • Author(s): 岩本 勉
  • Organizer: 第49回日本小児歯科学会大会
  • Place of Presentation: いわて県民情報交流センター・アイーナ(岩手県)
  • Year and Date: 2011-11-29
• [Presentation] 象牙芽細胞分化における細胞外マトリックス(ECM)の役割 (2011)
  • Author(s): 岩本 勉
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 長良川国際会議場(長野県)
  • Year and Date: 2011-10-01
• [Presentation] Pannexin 3 plays a critical role for odontoblast differentiation (2011)
  • Author(s): Iwamoto T, Sugawara Y, Ono M, Yamada A, Nakamura T, Fukumoto S
  • Organizer: American Society For Bone And Mineral Research (ASBMR) 2011 Annual Meeting
  • Place of Presentation: San Diego Convention Center, USA(サンディエゴ, 米国)
  • Year and Date: 2011-09-18
• [Presentation] 歯および軟骨細胞におけるPannexin 3 の役割 (2011)
  • Author(s): 岩本 勉, 福本 敏
  • Organizer: 第9回口腔医科学フロンティア学術集会
  • Place of Presentation: 九州大学歯学部本館1階(福岡県)
  • Year and Date: 2011-03-05
• [Presentation] New gap junctional protein pannexin 3 regulates tooth, cartilage and bone development (2011)
  • Author(s): Iwamoto T, Ono M, Arakaki M, Nakamura T, Yamada A, Fukumoto S
  • Organizer: Harvard-Forsyth-Tohoku Research Workshop
  • Place of Presentation: Belfer Case Study Room, CGIS South, Harvard University(ボストン, 米国)
  • Year and Date: 2011-01-07
• [Presentation] 象牙芽細胞分化におけるギャップ結合分子の発現および機能解析 (2010)
  • Author(s): 岩本 勉, 中村卓史, 吉崎恵悟, 山田亜矢, 福本 敏
  • Organizer: 第52回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: タワーホール船堀(千葉県)
  • Year and Date: 2010-09-22