• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2012 Fiscal Year Final Research Report

Analysis of the neuropathological mechanism by using calpain mutant mice

Research Project

  • PDF
Project/Area Number 22700379
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Nerve anatomy/Neuropathology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

TAKANO Jiro  独立行政法人理化学研究所, 神経蛋白制御研究チーム, 研究員 (60415213)

Project Period (FY) 2010 – 2012
Keywordsカルパイン / プロテアーゼ / カルシウムシグナル
Research Abstract

In this study, we have analyzed the motor abnoromality in calpain-1 and calpain-2 double knockout mice. Neuron-specific deleted knockout showed abnormalities in motor and/or visual capacity in Morris water maze test. Then, we identified several substrates of calpain-1 and calpain-2, which relate the functional abnormalities, by masspectrometic technique and the iTRAQ method. However, some of the substrates were not cleaved so much by calpain. Thus, we are trying to identify calpain substrates in cell culture system using calcium ionophores.

  • Research Products

    (17 results)

All 2013 2012 2011 2010

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Calpain-mediated ataxin-3 cleavage in the molecular pathogenesis of spinocerebellar ataxia type 3 (SCA3).2013

    • Author(s)
      Hubener J, Weber JJ, Richter C, Honold L, Weiss A, Murad F, Breuer P, Wullner U, Bellstedt P, Paquet-Durand F, Takano J, Saido TC, Riess O, Nguyen HP.
    • Journal Title

      Hum Mol Genet.

      Volume: 22(3) Pages: 508-18

    • Peer Reviewed
  • [Journal Article] A role for calpain-dependent cleavage of TDP-43 in amyotrophic lateral sclerosis pathology.2012

    • Author(s)
      Yamashita T, Hideyama T, Hachiga K, Teramoto S, Takano J, Iwata N, Saido TC, Kwak S.
    • Journal Title

      Nat Commun.

      Volume: 3 Pages: 130

    • Peer Reviewed
  • [Journal Article] Metabolic stress response implicated in diabetic retinopathy: the role of calpain, and the therapeutic impact of calpain inhibitor.2012

    • Author(s)
      Shanab AY, Nakazawa T, Ryu M, Tanaka Y, Himori N, Taguchi K, Yasuda M, Watanabe R, Takano J, Saido T, Minegishi N, Miyata T, Abe T, Yamamoto M.
    • Journal Title

      Neurobiol Dis.

      Volume: 48(3) Pages: 556-67

    • Peer Reviewed
  • [Journal Article] Calpastatin overexpression limits calpain-mediated proteolysis and behavioral deficits following traumatic brain injury.2012

    • Author(s)
      Schoch KM, Evans HN, Brelsfoard JM, Madathil SK, Takano J, Saido TC, Saatman KE.
    • Journal Title

      Exp Neurol.

      Volume: 236(2) Pages: 371-82

    • Peer Reviewed
  • [Journal Article] Mechanistic involvement of the calpain-calpastatin system in Alzheimer neuropathology.2012

    • Author(s)
      Higuchi M, Iwata N, Matsuba Y, Takano J, Suemoto T, Maeda J, Ji B, Ono M, Staufenbiel M, Suhara T, Saido TC.
    • Journal Title

      FASEB J.

      Volume: 26(3) Pages: 1204-17

    • Peer Reviewed
  • [Journal Article] Critical role of calpain in axonal damage-induced retinal ganglion cell death.2012

    • Author(s)
      Ryu M, Yasuda M, Shi D, Shanab AY, Watanabe R, Himori N, Omodaka K, Yokoyama Y, Takano J, Saido T, Nakazawa T.
    • Journal Title

      J Neurosci Res.

      Volume: 90(4) Pages: 802-15

    • Peer Reviewed
  • [Journal Article] Vital role of the calpain-calpastatin system for placental-integrity-dependent embryonic survival.2011

    • Author(s)
      Takano J, Mihira N, Fujioka R, Hosoki E, Chishti AH, Saido TC.
    • Journal Title

      Mol Cell Biol.

      Volume: 31(19) Pages: 4097-106

    • Peer Reviewed
  • [Journal Article] Potent amyloidogenicity and pathogenicity of Aβ43.2011

    • Author(s)
      Saito T, Suemoto T, Brouwers N, Sleegers K, Funamoto S, Mihira N, Matsuba Y, Yamada K, Nilsson P, Takano J, Nishimura M, Iwata N, Van Broeckhoven C, Ihara Y, Saido TC.
    • Journal Title

      Nat Neurosci.

      Volume: 14(8) Pages: 1023-32

    • Peer Reviewed
  • [Journal Article] Cleavage of the vesicular GABA transporter under excitotoxic conditions is followed by accumulation of the truncated transporter in nonsynaptic sites.2011

    • Author(s)
      Gomes JR, Lobo AC, Melo CV, Inacio AR, Takano J, Iwata N, Saido TC, de Almeida LP, Wieloch T, Duarte CB.
    • Journal Title

      J Neurosci.

      Volume: 31(12) Pages: 4622-35

    • Peer Reviewed
  • [Journal Article] Calpastatin, an endogenous calpain-inhibitor protein, regulates the cleavage of the Cdk5 activator p35 to p25.2011

    • Author(s)
      Sato K, Minegishi S, Takano J, Plattner F, Saito T, Asada A, Kawahara H, Iwata N, Saido TC, Hisanaga S.
    • Journal Title

      J Neurochem.

      Volume: 117(3) Pages: 504-15

    • Peer Reviewed
  • [Journal Article] Nicotinic acetylcholine receptor α1 promotes calpain-1 activation and macrophage inflammation in hypercholesterolemic nephropathy.2011

    • Author(s)
      Zhang G, Thomas AL, Marshall AL, Kernan KA, Su Y, Zheng Y, Takano J, Saido TC, Eddy AA.
    • Journal Title

      Lab Invest.

      Volume: 91(1) Pages: 106-23

    • Peer Reviewed
  • [Presentation] 胎盤発生における細胞内プロテアーゼ・カルパインの生理機能2012

    • Author(s)
      高野二郎
    • Organizer
      名古屋市立大学産科婦人科学生殖・遺伝セミナー
    • Place of Presentation
      愛知
    • Year and Date
      2012-07-07
  • [Presentation] 成体における細胞内プロテアーゼ・カルパインの機能2012

    • Author(s)
      高野二郎
    • Organizer
      第2回東北眼科疾患病態研究会
    • Place of Presentation
      宮城
    • Year and Date
      2012-02-28
  • [Presentation] 脳内カルパイン様プロテアーゼの解析2011

    • Author(s)
      高野二郎
    • Organizer
      日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-23
  • [Presentation] 遺伝子改変マウスから分かったカルパイン-1 とカルパイン-2 の機能2011

    • Author(s)
      高野二郎
    • Organizer
      Conference for Biosignal and Medcine
    • Place of Presentation
      三重
    • Year and Date
      2011-09-01
  • [Presentation] Genetic analysis of the calpain-calpastatin system using calpain-1, calpain-2, and calpastatin knockout mice2010

    • Author(s)
      Jiro Taknao
    • Organizer
      FASEB Summer Research Conference
    • Place of Presentation
      Carefree, Arizona
    • Year and Date
      2010-07-28
  • [Patent(Industrial Property Rights)] アルツハイマー病モデル動物およびその用途2010

    • Inventor(s)
      西道隆臣、岩田修永、斎藤貴志、末元隆寛、高野二郎
    • Industrial Property Rights Holder
      独立行政法人理化学研究所
    • Industrial Property Number
      国内出願,特願2006-170776
    • Acquisition Date
      2010-06-29

URL: 

Published: 2014-08-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi