2011 Fiscal Year Final Research Report
Visualization of spatiotemporal metabolic dynamics in tissue micro-region
Project/Area Number |
22710187
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Genome biology
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Research Institution | Kyushu University |
Principal Investigator |
MIURA Daisuke 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (40532627)
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Project Period (FY) |
2010 – 2011
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Keywords | メタボローム |
Research Abstract |
We successfully showed the applicability of a matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS) system for getting chemically diverse metabolite profiles on a single-mammalian cell. This ultrahighly sensitive MALDI-MS technique enabled a spatially resolved detection of a broad range of metabolites including nucleotides, cofactors, phosphorylated sugars, amino acids, lipids, and carboxylic acids with their unique distributions. A combination of MS imaging and metabolic pathway analysis visualized a spatiotemporal behavior of metabolites in the central metabolic pathway regulated by an ischemia reperfusion in rat brain. Furthermore, we developed a novel strategy for determining the elemental composition of organic compounds using the peak ratio of isotopic fine structure observed by ultrahigh mass resolution mass spectrometric data.
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[Journal Article] Ultra-highly sensitive in situ metabolomic imaging for visualizing spatiotemporal metabolic behaviors2010
Author(s)
Miura, D., Fujimura, Y., Yamato, M., Hyodo, F., Tachibana, H., Utsumi, H., and Wariishi.
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Journal Title
AnaL Chem
Volume: 82
Pages: 9789-9796
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[Presentation] Visualization of Spatiotemporal Metabolic Behaviors by in situ Metabolomic Imaging with MALDI-MS0
Author(s)
Miura, D., Fujimura, Y., Yamato, M., Hyodo, F., Tachibana, H., Utsumi, H., and Wariishi.
Organizer
58th ASMS Conference
Place of Presentation
Salt Lake, Utah, USA
Year and Date
00000525
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