Visualization of spatiotemporal metabolic dynamics in tissue micro-region

2011 Fiscal Year Final Research Report

• Project/Area Number: 22710187
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genome biology
• Research Institution: Kyushu University
• Principal Investigator: MIURA Daisuke 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (40532627)
• Project Period (FY): 2010 – 2011
• Keywords: メタボローム

Research Abstract

We successfully showed the applicability of a matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS) system for getting chemically diverse metabolite profiles on a single-mammalian cell. This ultrahighly sensitive MALDI-MS technique enabled a spatially resolved detection of a broad range of metabolites including nucleotides, cofactors, phosphorylated sugars, amino acids, lipids, and carboxylic acids with their unique distributions. A combination of MS imaging and metabolic pathway analysis visualized a spatiotemporal behavior of metabolites in the central metabolic pathway regulated by an ischemia reperfusion in rat brain. Furthermore, we developed a novel strategy for determining the elemental composition of organic compounds using the peak ratio of isotopic fine structure observed by ultrahigh mass resolution mass spectrometric data.

Research Products

• [Journal Article] Optimization of extraction method for GC-MS based metabolomics for filamentous fungi (2012)
  • Author(s): Madla, S., Miura, D. and Wariishi, H.
  • Journal Title: J. Microb. Biochem. Technol Volume: 4 Pages: 5-9
• [Journal Article] Potential Applicability of MALDI-MS for Low-Molecular-Weight Pesticide Determination (2012)
  • Author(s): Madla, S., Miura, D. and Wariishi, H.
  • Journal Title: AnaL Sci Volume: 28 Pages: 301-303
• [Journal Article] Ultra-highly sensitive in situ metabolomic imaging for visualizing spatiotemporal metabolic behaviors (2010)
  • Author(s): Miura, D., Fujimura, Y., Yamato, M., Hyodo, F., Tachibana, H., Utsumi, H., and Wariishi.
  • Journal Title: AnaL Chem Volume: 82 Pages: 9789-9796
• [Journal Article] A Strategy for the Determination of the Elemental Composition by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Based on Isotopic Peak Ratios (2010)
  • Author(s): Miura, D., Tsuji, Y., Takahashi, K., Wariishi, H., and Saito, K.
  • Journal Title: Anal. Chem Volume: 82 Pages: 5887-5891
• [Journal Article] MALDI-MS-Based High-Throughput Metabolite Analysis for Intracellular Metabolic Dynamics (2010)
  • Author(s): Yukihira, D., Miura, D., Saito, K., Takahashi, K., and Wariishi, H.
  • Journal Title: AnaL Chem Volume: 82 Pages: 4278-4282
• [Journal Article] Highly Sensitive MALDI-Mass Spectrometry for High-throughput Metabolic Profiting (2010)
  • Author(s): Miura, D., Fujimura, Y., Tachibana, H., and Wariishi, H.
  • Journal Title: Anal. Chem Volume: 82 Pages: 498-504
• [Presentation] MALDI-MSを用いた高時間分解代謝動態解析法 (2011)
  • Author(s): 三浦大典
  • Organizer: 第6回メタボロームシンポジウム
  • Place of Presentation: 大阪大学
  • Year and Date: 2011-11-14
• [Presentation] High-throughput Metabolicprofitingg and in situ Metabolite Imaging by MALDI mass spectrometry (2011)
  • Author(s): Miura, D., Yukihir, D., Fujimura, Y., and Wariishi.
  • Organizer: 59th ASMS Conference
  • Place of Presentation: Denver, USA
  • Year and Date: 2011-06-08
• [Presentation] 質量分析による代謝動態の可視化 (2010)
  • Author(s): 三浦大典
  • Organizer: 第49回電子スピンサイエンス学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2010-11-13
• [Presentation] MALDI-MS-based Metabolite Analysis for High-throughput Metabolic Dynamics and in situ Metabolomics Imaging (2010)
  • Author(s): Miura, D., Yukihir, D., Fujimura, Y., Tachibana, H., and Wariishi.
  • Organizer: Metabolomics 2010
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 2010-06-26
• [Presentation] Visualization of Spatiotemporal Metabolic Behaviors by in situ Metabolomic Imaging with MALDI-MS (0)
  • Author(s): Miura, D., Fujimura, Y., Yamato, M., Hyodo, F., Tachibana, H., Utsumi, H., and Wariishi.
  • Organizer: 58th ASMS Conference
  • Place of Presentation: Salt Lake, Utah, USA
  • Year and Date: 00000525
• [Remarks]
  • URL: http://redoxnavi.kyushu-u.ac.jp/group2/