2011 Fiscal Year Final Research Report
Correlation analysis by electrochemical detection of gene methylation detection and telomerase activity of tumor cells
| Project/Area Number |
22750072
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
SHINOBU Sato 九州工業大学, 大学院・工学研究院, 助教 (80510677)
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| Project Period (FY) |
2010 – 2011
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| Keywords | テロメラーゼ / TERT / DNAメチレーション / 電気化学 |
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| Research Abstract |
Telomerase is an enzyme which can elongate telomere DNAs and is a good candidate for a cancer marker because of its specific expression in cancer cells. The promoter region of the hTERT gene, encoding for the catalytic subunit of human telomerase, has been located in a CpG island and may therefore be regulated at least in part by DNA methylation. We analyzed the methylation status of five CpG sites within the hTERT promoter core region by FND-based electrochemical hybridization assay and electrochemical telomerase activity assay in lysates of oral cancer patients.
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