Effect of molecular chaperone on misfolding protein aggregation

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770117
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ZAKO Tamotsu 独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (50399440)
• Project Period (FY): 2010 – 2011
• Keywords: アミロイド / 分子シャペロンタンパク質 / ミスフォールディングタンパク質凝集 / プレフォルディン / 可溶性オリゴマー / 細胞毒性

Research Abstract

Protein misfolding disease, such as Alzheimer's' disease(AD), is caused by misfolded protein aggregates that induce molecular stress and interfere with cellular function. We examined effect of molecular chaperone prefoldin(PFD) on aggregation of amyloid beta and poly glutamine protein that cause AD and Huntington disease, respectively. We found that PFD could inhibit their aggregation in vitro.

Research Products

• [Journal Article] Immobilized Insulin Amyloid Enhances Cell Adhesion and Proliferation due to Interaction with Fibronectin (2011)
  • Author(s): Sakono M, Akiyama S, Zako T., Sakaki S, Waku T, Tanaka N, Maeda M
  • Journal Title: Chem. Lett Volume: 40 Pages: 315-317
  • Peer Reviewed
• [Journal Article] Application of two morphologically different fibrillar and filamentous insulin amyloids as a biomaterial for cell culture surfaces (2011)
  • Author(s): Sakono M, Akiyama S, Zako T., Sakaki S, Waku T, Tanaka N, Maeda M
  • Journal Title: React. Funct. Polymer Volume: 71 Pages: 324-328
  • Peer Reviewed
• [Journal Article] Size-selective recognition of gold nanoparticles by a molecular chaperone (2010)
  • Author(s): Sakono M, Zako T., Drakulic S, Valpuesta J. M, Yohda M and Maeda M
  • Journal Title: Chem. Phys. Lett Volume: 501 Pages: 108-112
  • Peer Reviewed
• [Journal Article] Analysis of interaction mode between hyperthermophilic archaeal group II chaperonin and prefoldin using a platform of chaperonin oligomers of various subunit arrangements (2010)
  • Author(s): Sahlan M., Kanzaki T., Zako T., Maeda M. and Yohda M.
  • Journal Title: Biochimica et Biophysica Acta-Proteins and Proteomics Volume: 1804 Pages: 1810-1816
  • Peer Reviewed
• [Journal Article] Thermodynamic Characterization of the Interaction between Prefoldin and Group II Chaperonin (2010)
  • Author(s): Sahlan M., Zako T., Tai Phan The, Ohtaki A., Noguchi K., Maeda M., Miyatake H., Dohmane N. and Yohda M.
  • Journal Title: J. Mol. Biol Volume: 399 Pages: 628-636
  • Peer Reviewed
• [Journal Article] Bio-Supramolecular Photochirogenesis with Molecular Chaperone : Enantiodifferentiating Photocyclodimerization of 2-Anthracenecarboxylate Mediated by Prefoldin (2010)
  • Author(s): Bando K., Zako T., Sakono M., Maeda M., Wada T., Nishijima M., Fukuhara G., Yang C., Mori T., Pace TCS., Bohne C. and Inoue Y.
  • Journal Title: Photochem. & Photobiol. Sci Volume: 9 Pages: 655-60
  • Peer Reviewed
• [Journal Article] Amyloid oligomers : Formation and toxicity of Abeta oligomers (2010)
  • Author(s): Sakono M. and Zako T.
  • Journal Title: FEBS J. Volume: 277 Pages: 1348-1358
  • Peer Reviewed
• [Journal Article] Hyperthermophilic archaeal prefoldin shows refolding activity at low temperature (2010)
  • Author(s): Zako T., Banba S., Sahlan M., Sakono M., Terada N., Yohda M. and Maeda M.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 391 Pages: 467-470
  • Peer Reviewed
• [Presentation] Function and application of protein molecules and bio-nanoparticles (2011)
  • Author(s): Tamotsu Zako and Mizuo Maeda
  • Organizer: Japan-Canada Nanotechnology Workshop
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2011-11-22
• [Presentation] Development of new photon counting histogram method towards precise analysis of oligomerization (2011)
  • Author(s): Naofumi Terada, Tamotsu Zako, Masafumi Sakono and Mizuo Maeda
  • Organizer: 第49回生物物理年会
  • Place of Presentation: 姫路
  • Year and Date: 2011-09-17
• [Presentation] Noodle'-like filaments of bovine insulin andβ2 microglobulin were non-cytotoxic due to abbreviated cell binding (2011)
  • Author(s): Tamotsu Zako, Masafumi Sakono, Takahiro Kobayashi, K. Peter R. Nilsson, Per Hammarstrom, Mikael Lindgren and Mizuo Maeda
  • Organizer: EMBO meeting 2011
  • Place of Presentation: Vienna
  • Year and Date: 2011-09-12
• [Presentation] The molecular chaperone prefoldin can capture aggregation prone states of A-beta and might play a role in the progress of Alzheimers disease (2010)
  • Author(s): Karin Sorgjerd, Tamotsu Zako, Masafumi Sakono, Peter Stirling, Michel Leroux, Mizuo Maeda
  • Organizer: Pacifichem2010
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-19
• [Presentation] Toxic amyloid oligomer detection system using molecular chaperone (2010)
  • Author(s): Masafumi Sakono, Tamotsu Zako, Masafumi Yohda and Mizuo Maeda
  • Organizer: Pacifichem2010
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-18
• [Presentation] Supramolecular photochirogenesis with biomolecular hosts (2010)
  • Author(s): Y. Inoue, M. Nishijima, C. Yang, T. Mori, T. Wada, K. Bando, T. Zako, M. Sakon, M. Maeda, T. Pace, C. Bohne
  • Organizer: Pacifichem2010
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-16
• [Presentation] 分子シャペロンPrefoldinは変異ハンチンチンのオリゴマーおよび凝集体形成を阻害して細胞死を減少させる (2010)
  • Author(s): 田代絵梨佳,武藤秀樹,座古保,宮澤誠,北浦廣剛,北村朗,久保田広志,前田瑞夫,金城政孝,有賀寛芳
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-06
• [Presentation] Molecular chaperone prefoldin blocks the formation of mutant Huntingtin oligomer and inclusion body, resulting in reduction of its cytotoxity (2010)
  • Author(s): Erika Tashiro, Hideki Muto, Tamotsu Zako, Makoto Miyazawa, Hirotake Kitaura, Akira Kitamura, Hiroshi Kubota, Mizuo Maeda, Masataka Kinjo, Hiroyoshi Ariga
  • Organizer: Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-16
• [Presentation] Amyloid Beta Oligomer Studied By photon counting histogram (2010)
  • Author(s): Naofumi Terada, Tamotsu Zako, Masafumi Sakono and Mizuo Maeda
  • Organizer: 第48回日本生物物理学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-20
• [Presentation] Formation of non-toxic Abeta amyloids by small heat shock protein(sHsp) under stress condition (2010)
  • Author(s): Tamotsu Zako, Masafumi Sakono, Arata Utsum, Chika Sugino, Masafumi Yohda, Mizuo Maeda
  • Organizer: EMBO meeting
  • Place of Presentation: Barcelona Spain
  • Year and Date: 2010-09-06
• [Presentation] 分子シャペロンをめぐる分子間相互作用の解析 (2010)
  • Author(s): 養王田正文、阿部哲也、Sahlan Muhamad, Phan The tai,神埼太郎、岡俊彦、座古保、前田瑞夫
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-06-17
• [Presentation] ポリグルタミンタンパク質凝集における分子シャペロンプレフォルディンの役割 (2010)
  • Author(s): 伊藤禎宣、座古保、Karin Sorgjerd、寺田尚史、田代絵梨佳、北村朗、久保田広志、武藤秀樹、金城政孝、有賀寛芳、前田瑞夫
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-06-16
• [Presentation] The Molecular Chaperone Prefoldin can Capture Aggregation Prone States of A-Beta and Might Play a Role in the Progress of Alzheimer's Disease (2010)
  • Author(s): Karin Sorgjerd, Tamotsu Zako, Masafumi Sakono, Peter Stirling, Michel Leroux, Mizuo Maeda
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: 仙台
  • Year and Date: 2010-05-24
• [Presentation] Formation of non-toxic Abeta fibrils by small heat shock protein(sHsp) under stress condition (2010)
  • Author(s): Tamotsu Zako, Masafumi Sakono, Arata Utsum, Chika Sugino, Masafumi Yohda, Mizuo Maeda
  • Organizer: Cold Spring Harbor Meeting Molecular Chaperons & Stress responses
  • Place of Presentation: New York, USA
  • Year and Date: 2010-05-07