Mechanisms of chloroplast degradation by two-different autophagic pathways in senescent leaves

2011 Fiscal Year Final Research Report

• Project/Area Number: 22780054
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant nutrition/Soil science
• Research Institution: Tohoku University
• Principal Investigator: ISHIDA Hiroyuki 東北大学, 大学院・農学研究科, 准教授 (60312625)
• Project Period (FY): 2010 – 2011
• Keywords: 老化 / 植物 / オートファジー / 葉緑体

Research Abstract

I studied membrane dynamics and related genes among chloroplast autophagy that occurs in senescent leaves. Results suggested that (i)unlike RCBs, chloroplasts are transferred into the vacuolar lumen by invagination of tonoplasts ; (ii)in addition to ATG4, ATG2, 5, 7, and 10 are required for the chloroplast autophagy.

Research Products

• [Journal Article] The changes of leaf carbohydrate contents as a regulator of autophagic degradation of chloroplasts via Rubisco-containing bodies during leaf senescence (2011)
  • Author(s): Izumi, M., Ishida, H.
  • Journal Title: Plant Signal Behav Volume: 6 Pages: 685-687
  • Peer Reviewed
• [Journal Article] The autophagic degradation of chloroplasts via Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis (2010)
  • Author(s): Izumi, M., Wada, S., Makino, A., Ishida, H.
  • Journal Title: Plant Physiol Volume: 154 Pages: 1196-1209
  • Peer Reviewed
• [Presentation] シロイヌナズナ葉の炭素制限環境におけるRCB/オートファジー系の生理的意義について (2012)
  • Author(s): 泉正範、牧野周、石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-16
• [Presentation] 葉の老化と葉緑体のオートファジー (2011)
  • Author(s): 石田宏幸、吉本光希、和田信也、泉正範、小野祐樹、牧野周
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2011-09-06
• [Presentation] オートファジーによる葉緑体タンパク質の分解 (2011)
  • Author(s): 石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2011-03-22
• [Presentation] シロイヌナズナにおけるRBCS1Aおよび3Bの変異が葉のRubisco量に与える影響の解析 (2011)
  • Author(s): 泉正範、角田穂奈美、鈴木雄二、牧野周、石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2011-03-22
• [Presentation] シロイヌナズナにおけるRubisco-containing body (RCB)形成の栄養要因に対する応答の解析 (2010)
  • Author(s): 泉正範、牧野周、石田宏幸
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 北海道(札幌市)
  • Year and Date: 2010-09-07
• [Presentation] A possible role for plastid stromules in the autophagic transfer of stromal proteins to the vacuole (2010)
  • Author(s): Ishida, H., Izumi, M., Yoshimoto, K., Hanson, M. R., Makino, A.
  • Organizer: Gordon Research conference ' Mitochondria and Chloroplasts'
  • Place of Presentation: バルガ, イタリア
  • Year and Date: 2010-07-11
• [Presentation] The autophagic degradation of chloroplasts via Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis (2010)
  • Author(s): Izumi, M., Makino, A., Ishida, H.
  • Organizer: Gordon Research conference ' Mitochondria and Chloroplasts'
  • Place of Presentation: バルガ, イタリア
  • Year and Date: 2010-07-11
• [Presentation] The impact of autophagic degradation in chloroplasts and other organelles during leaf senescence of Arabidopsis (2010)
  • Author(s): Wada, S., Ishida, H., Yoshimoto, K., Ohsumi, Y., Makino, A.
  • Organizer: Gordon Research conference ' Mitochondria and Chloroplasts'
  • Place of Presentation: バルガ, イタリア
  • Year and Date: 2010-07-11
• [Presentation] A key factor for the autophagy of chloroplasts ; the production of Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis (2010)
  • Author(s): Izumi M., Makino A., Ishida H.
  • Organizer: The 21st International Conference on Arabidopsis Research
  • Place of Presentation: 横浜市
  • Year and Date: 2010-06-06
• [Book] Rubisco turnover and nitrogen in a leaf. Nitorgen assimilation in plants. Research Sinpost (2010)
  • Author(s): Ishida, H., Suzuki, Y., Makino A.
  • Total Pages: 277-285
  • Publisher: Research Sinpost
• [Book] Protein turnover in grass leaves. Advances in Botanical Research (2010)
  • Author(s): Irving, L. J., Suzuki, Y., Ishida, H., Makino, A., Kader, J. C. and Delseny, M. Eds., Elsevier
  • Total Pages: 139-182
  • Publisher: Elsevier