What establishes the symbiotic relationship between human and bifidobacteria?"A bifidogenic effects of human milk oligosaccharides"

2011 Fiscal Year Final Research Report

• Project/Area Number: 22780072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: KATAYAMA Takane 石川県立大学, 生物資源環境学部, 准教授 (70346104)
• Project Period (FY): 2010 – 2011
• Keywords: ヒト母乳オリゴ糖 / ビフィズス菌 / 共生 / グリコシダーゼ

Research Abstract

The breast-fed infant intestine is often colonized by particular bifidobacteria, and human milk oligosaccharides(HMOs) are considered to be bifidogenic ; however, the precise mechanism underlying it remains unresolved. We have isolated and characterized various glycosidases that act on HMOs from Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. longum and revealed how these enzymes are involved in the degradation of HMOs by these bifidobacteria. These results provide us with essential and basic understanding of physiology of bifidogenic effects of HMOs, and suggested co-evolution of bifidobacteria with humans. We also developed an efficient method of enzymatic synthesis of 2'-fucosyllactose, the most abundant component of HMOs, by introducing a glycosynthase technology into 1, 2-α-L-fucosidase.

Research Products

• [Journal Article] Bifidobacterium longum subsp. infantis uses two differentβ-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharides (2012)
  • Author(s): Yoshida E, Sakurama H, Kiyohara M, Nakajima M, Kitaoka M, Ashida H, Hirose J, Katayama T, Yamamoto K, Kumagai H.
  • Journal Title: Glycobiology Volume: 22 Pages: 361-368
  • URL: http://glycob.oxfordjournals.org/
  • Peer Reviewed
• [Journal Article] Physiology of the consumption of human milk oligosaccharides by infant-gut associated bifidobacteria (2011)
  • Author(s): Asakuma S, Hatakeyama E, Urashima T, Yoshida E, Katayama T, Yamamoto K, Kumagai H, Ashida H, Hirose J, Kitaoka M.
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 34583-34592
  • URL: http://www.jbc.org/
  • Peer Reviewed
• [Journal Article] An exo-α-sialidase from bifidobacteria involved in the degradation of sialyloligosaccharides in human milk and intestinal glycoconjugates (2011)
  • Author(s): Kiyohara M, Tanigawa K, Chaiwangsri T, Katayama T, Ashida H, Yamamoto K.
  • Journal Title: Glycobiology Volume: 21 Pages: 437-447
  • URL: http://glycob.oxfordjournals.org/
  • Peer Reviewed
• [Journal Article] Crystal structures of phosphoketolase : thiamine diphosphate-dependent dehydration mechanism (2010)
  • Author(s): Suzuki R, Katayama T, Kim BJ, Wakagi T, Shoun H, Ashida H, Yamamoto K, and Fushinobu S.
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 34279-34287
  • URL: http://www.jbc.org/
  • Peer Reviewed
• [Journal Article] Overexpression, crystallization and preliminary X-ray analysis of xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from Bifidobacterium breve (2010)
  • Author(s): Suzuki R, Kim BJ, Shibata T, Iwamoto Y, Katayama T, Ashida H, Wakagi T, Shoun H, Fushinobu S, and Yamamoto K.
  • Journal Title: Acta Crystallographica Section F. Structural Biology and Crystallization Communications Volume: 66 Pages: 941-943
  • URL: http://journals.iucr.org/f/
  • Peer Reviewed
• [Journal Article] Cooperation ofβ-galactosidase andβ-N-acetyl-hexosaminidase from bifidobacteria in assimilation of human milk oligosaccharides with type 2 structure (2010)
  • Author(s): Miwa M, Horimoto T, Kiyohara M, Katayama T, Kitaoka M, Ashida H, and Yamamoto K.
  • Journal Title: Glycobiology Volume: 20 Pages: 1402-1409
  • URL: http://glycob.oxfordjournals.org/
  • Peer Reviewed
• [Presentation] ビフィズス菌のヒトミルクオリゴ糖代謝経路 (2011)
  • Author(s): 北岡本光, 西本完, 片山高嶺, 清原正志, 和田潤, 芦田久, 山本憲二, 浦島匡
  • Organizer: 63回日本生物工学会大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-09-26
• [Presentation] ビフィズス菌によるヒトミルクオリゴ糖の分解-培養上清におけるオリゴ糖濃度の経時変化- (2011)
  • Author(s): 片山高嶺, 朝隈貞樹, 畑山恵美, 浦島匡, 吉田永史奈, 熊谷英彦, 芦田久, 廣瀬潤子, 北岡本光, 山本憲二
  • Organizer: 63回日本生物工学会大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-09-26
• [Presentation] A UNIQUE PATHWAY OF BIFIDOBACTERIA FOR THE DEGRADATION OF HUMAN MILK OLIGOSACCHARIDES (2011)
  • Author(s): Takane Katayama, Hisashi Ashida, Motomitsu Kitaoka, Shinya Fushinobu, Hidehiko Kumagai, and Kenji Yamamoto
  • Organizer: International Union of Microbiological Societies 2011Congress
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-09
• [Presentation] ビフィズス菌ヒトミルクオリゴ糖分解酵素を利用したフコシルオリゴ糖の調製 (2011)
  • Author(s): 片山高嶺, 本多裕司, 熊谷英彦, 山本憲二
  • Organizer: 日本農芸化学会2011年度大会シンポジウム
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-28
• [Presentation] Bifidobacterium longum subsp. infantis ATCC15697株のヒトミルクオリゴ糖分解酵素 (2011)
  • Author(s): 吉田永吏奈, 中島将博, 北岡本光, 廣瀬潤子, 清原正志, 片山高嶺, 山本憲二, 熊谷英彦
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-27