2011 Fiscal Year Final Research Report

Studies on the assembly of the proteasome

Research Project

Project/Area Number	22790060
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Biological pharmacy
Research Institution	The University of Tokyo
Principal Investigator	KANEKO Takeumi 東京大学, 大学院・薬学系研究科, 助教 (80508135)
Research Collaborator	MURATA Shigeo 東京大学, 大学院・薬学系研究科, 教授 (20344070)
Project Period (FY)	2010 – 2011
Keywords	プロテアソーム / ユビキチン / タンパク質分解 / 分子集合
Research Abstract	The 26S proteasome is an about 2. 5 MDa large protein complex consisting of a catalytic 20S proteasome and one or two 19S regulatory particles. More than 70 subunits are gathered to form the 26S proteasome. To reveal how such a large complex is constructed, we focused on the assembly mechanism of the lid subcomplex, which has not yet been examined precisely. Our research revealed that the lid is assembled in a well-ordered way, and the assembly of the lid is completed by the integration of Rpn12.