2011 Fiscal Year Final Research Report
The study of the insulin receptor cleavage and its clinical significance
| Project/Area Number |
22790317
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
YUASA Tomoyuki 徳島大学, 疾患酵素学研究センター, 准教授 (50304556)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 可溶性インスリン受容体 / インスリン抵抗性 / 糖尿病 |
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| Research Abstract |
Soluble insulin receptor(sIR) was found to exist in human plasma and its amount was elevated in patients with diabetes. In this study, we have developed an in vitro cell model system, which mimics the sIR generation in humans, by using human cell line and an ultra-sensitive enzyme immunoassay for sIR. The sIR generation was positively correlated with the glucose concentration in the medium as well as the duration of the cell culture. In contrast, the identical osmolarity with sorbitol, mannitol or sodium chloride solutions did not stimulate the sIR generation. Therefore, glucose is thought to work as a stimulant for sIR after it is absorbed and metabolized in the cell.
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