2011 Fiscal Year Final Research Report

Comprehension of epidemiologic information and genomics of Mycobacterium tuberculosis clinical strains causing putative nationwide proliferation

Research Project

Project/Area Number	22790585
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Public health/Health science
Research Institution	Osaka City Institute of Public Health and Environmental Sciences
Principal Investigator	WADA Takayuki 大阪市立環境科学研究所, 研究主任 (70332450)
Project Period (FY)	2010 – 2011
Keywords	感染症 / ゲノム / 衛生 / 社会医学 / 分子疫学 / 公衆衛生 / 結核 / 細菌
Research Abstract	Genotyping of clinical isolates of Mycobacterium tuberculosis has been used to estimate their putative transmission routes. In this study, we focused on two genotypes of M. tuberculosis, pECT04 and pECT07, detected occasionally from broad areas in Japan. Totally twelve clinical isolates of the genotypes were genomically sequenced for comparative analysis. Results show that phylogenetic relation among pECT07 isolates was not associated with their geographic origins, which suggests their complicated transmission routes around Japan. Contrary to pECT04, many SNPs characteristic to two isolates of pECT04 were identified. These results mean that comparative genomics of clinical isolates can provide deep traceability of transmission of M. tuberculosis that has never achieved with present genotyping methods.