Exploration of the new therapeutic target molecule for treatmentresistant mood disorders through elucidation of the molecular mec hanism of adult hippocampal neurogenesis

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791102
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Psychiatric science
• Research Institution: Hokkaido University
• Principal Investigator: BOKU Syuken 北海道大学, 北海道大学病院, 助教 (60455665)
• Project Period (FY): 2010 – 2011
• Keywords: 気分障害 / 成体海馬神経細胞新生 / FGF / GSK-3β / β-catenin / ROCK

Research Abstract

We have already shown that ROCK2 is involved in DEX-induced decrease of bFGF-induced proliferation of adult rat dentate gyrus-derived neural precursor cells(ADP) through regulating he activity of GSK3βand that bFGF and DEX reciprocally regulate the expression of ROCK2. Therefore, ROCK2 may play a role in adult neurogenesis in dentate gyrus. However, it remains unclear whether ROCK2 actually regulate in vivo neurogenesis in adult dentate gyrus. Here we examined the expression of ROCK2 in adult dentate gyrus with in situ hybridization. In addition, we tried to prepare the overexpression system of ROCK2 with retroviral vector and lentiviral vector.

Research Products

• [Journal Article] Involvement of CaMKIV in neurogenic effect with chronic fluoxetine treatment (2012)
  • Author(s): Song N, Nakagawa S, Izumi T, Toda H, Kato A, Boku S, Inoue T, Sakagami H, Li X, Koyama T
  • Journal Title: Int J Neuropsychopharmacology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Mood stabilizers commonly restore staurosporine-induced increase of p53 expression and following decrease of Bcl-2 expression in SH-SY5Y cells (2012)
  • Author(s): Song N, Boku S, Nakagawa S, Kato A, Toda H, Takamura N, Omiya Y, Kitaichi Y, Inoue T, Koyama T
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry, Epub ahead of print
  • Peer Reviewed
• [Journal Article] Noradrenaline increases neural precursor cells derived from adult rat dentate gyrus through beta2 receptor (2012)
  • Author(s): Masuda T, Nakagawa S, Boku S, Nishikawa H, Takamura N, Kato A, Inoue T, Koyama T
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry Volume: 36 Pages: 44-51
  • Peer Reviewed
• [Journal Article] Effects of combined treatment with clorgyline and selegiline on extracellular noradrenaline and serotonin levels (2012)
  • Author(s): Kitaichi Y, Inoue T, Nakagawa S, Boku S and Koyama T
  • Journal Title: Acta Neuropsychiatr, Accepted manuscript online
  • DOI: DOI:10.1111/j.1601-5215.2012.00652.x
  • Peer Reviewed
• [Journal Article] 5-HT depletion, but not 5-HT1A antagonist, prevents the anxiolytic-like effect of citalopram in rat contextual conditioned fear stress model (2012)
  • Author(s): Masuda T, Nishikawa H, Inoue T, Toda H, Nakagawa S, Boku S, Koyama T
  • Journal Title: Acta Neuropsychiatr, Accepted manuscript online
  • DOI: DOI:10.1111/j.1601-5215.2012.00669.x
  • Peer Reviewed
• [Journal Article] Sertraline treatment of patients with major depressive disorder who failed initial treatment with paroxetine or fluvoxamine (2012)
  • Author(s): Inoue T, Honda M, Kawamura K, Tsuchiya K, Suzuki T, Ito K, Matsubara R, Shinohara K, Ishikane T, Sasaki K, Boku S, Fujisawa D, Ono Y, Koyama T
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry Pages: 22504727
  • Peer Reviewed
• [Journal Article] Effects of mood stabilizers on adult dentate gyrus-derived neural precursor cells (2011)
  • Author(s): Boku S, Nakagawa S, Masuda T, Nishikawa S, Kato A, Toda H, Song N, Kitaichi Y, Inoue T, Koyama T
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry Volume: 35 Pages: 111-117
  • Peer Reviewed
• [Journal Article] Olanzapine augmentation of milnacipran for stage 2 treatment-resistant major depression an open study (2011)
  • Author(s): Boku S, Inoue T, Honma H, Matsubara S, Nakagawa S, Koyama T
  • Journal Title: Hum Psychopharmacol Volume: 26 Pages: 237-241
  • Peer Reviewed
• [Journal Article] Retrieval of conditioned fear activates the basolateral and intercalated nucleus of amygdala (2011)
  • Author(s): Izumi T, Boku S, Shinmin W, Inoue T, Konno K, Yamaguchi T, Yoshida T, Matsumoto M, Watanabe M, Koyama T, Yoshioka M
  • Journal Title: J Neurosci Res Volume: 89(5) Pages: 773-790
  • Peer Reviewed
• [Journal Article] Sertraline increases extracellular levels not only of serotonin, but also of dopamine in the nucleus accumbens and striatum of rats (2010)
  • Author(s): Kitaichi Y, Inoue T, Nakagawa S, Boku S, Kakuta A, Izumi T, Koyama T
  • Journal Title: Eur J Pharmacol Volume: 647 Pages: 90-96
  • Peer Reviewed
• [Presentation] Rho-associated coiled-coil kinase(ROCK2) regulates bFGF-induced proliferation of SH-SY5Y cells through GSK-3βandβ-catenin pathway (2011)
  • Author(s): Boku S, Nakagawa S, Toda H, et al
  • Organizer: Society for Neuroscience 41^<th> annual meeting
  • Place of Presentation: Washington DC
  • Year and Date: 20110000
• [Presentation] 気分安定薬はSH-SY5Y細胞においてstaurosporineによるp53の発現増加およびBcl-2の発現減少を抑制させる (2011)
  • Author(s): 朴秀賢、宋寧、中川伸、加藤亜紀子、高村直樹、戸田裕之、井上猛、小山司
  • Organizer: 第30回躁うつ病懇話会
  • Place of Presentation: 京都(ガーデンホテル京都)
  • Year and Date: 20110000
• [Book] 気分安定薬と成体海馬歯状回神経細胞新生.気分障害の薬理・生化学-総括と新たなる挑戦- (2012)
  • Author(s): 朴秀賢
  • Total Pages: 125-127
  • Publisher: 医薬ジャーナル