2011 Fiscal Year Final Research Report
Identification of tumor antigen derived peptides having the potential to promise candidates for cancer vaccine therapy from prostate cancer patients研究代表者南高文(MINAMI TAKAFUMI)
| Project/Area Number |
22791505
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 前立腺癌 / 癌ワクチン / HLA-A3 supertype alleles |
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| Research Abstract |
Peripheral blood mononuclear cells(PBMCs) were obtained from prostate cancer patients. The PBMCs were prepared by Ficoll-Conray density gradient centrifugation. The expression of HLA-A11,-A31, and-A33 molecules on the PBMCs was determined by flow cytometry using these antibodies-anti-HLA-A11,-A31,-A33-and FITC-conjugated anti-mouse IgG monoclonal antibodies. 3 prostate cancer-related antigens prepared based on the binding motifs to the MHC-class I alleles. It is predicted as a peptide candidate that be identified induction of specific CTL with EZHp_<733-741> peptide in CTL assay, and can apply to a cancer vaccine treatment.
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